Rapid characterization of N-linked glycans from secreted and gel-purified monoclonal antibodies using MALDI-ToF mass spectrometry

Biotechnology and Bioengineering

Volumn 107, Issue 5, 2010, Pages 902-908

  Hansen, Rasmus ^a   Dickson, Alan J ^a   Goodacre, Royston ^a   Stephens, Gill M ^b   Sellick, Christopher A ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Antibody; Deglycosylation; IgG1; IgG4; In gel analysis; MALDI ToF MS

Indexed keywords

DEGLYCOSYLATION; IGG1; IGG4; IN-GEL ANALYSIS; MALDI TOF MS;

CELL CULTURE; DESALINATION; ESTERIFICATION; GELS; GLYCOSYLATION; INDUCTIVELY COUPLED PLASMA; MANAGEMENT; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; SURFACE PLASMON RESONANCE;

PURIFICATION;

GLYCAN; MONOCLONAL ANTIBODY;

DEGLYCOSYLATION; GLYCOSYLATION; LETTER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PURIFICATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; POLYSACCHARIDES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 77958599435     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22879     Document Type: Letter

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