A novel mixing device for the reproducible generation of nonviral gene therapy formulations

BioTechniques

Volumn 49, Issue 3, 2010, Pages 666-668

  Davies, Lee A ^a,d   Nunez Alonso, Graciela A ^a,d   Hebel, Henry L ^b   Scheule, Ron K ^c   Cheng, Seng H ^c   Hyde, Stephen C ^a,d   Gill, Deborah R ^a,d  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b VGXI   (United States)

^c GENZYME CORPORATION   (United States)

^d UK Cystic Fibrosis Consortium ^*  (United Kingdom)

Author keywords

Gene therapy; Lipoplexes; Nonviral formulations; Polyplexes

Indexed keywords

LIPOPLEX; PLASMID DNA;

ARTICLE; GENE EXPRESSION; HYDRODYNAMICS; LMD 2 DEVICE; MIXER; NONVIRAL GENE THERAPY; POLYMERIZATION; REPRODUCIBILITY; ZETA POTENTIAL;

ANIMALS; CATIONS; DNA; EQUIPMENT DESIGN; GENE EXPRESSION; GENE THERAPY; LIPIDS; MICE; MICE, INBRED BALB C; PLASMIDS;

EID: 77958580393     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113498     Document Type: Article

References (9)

1. Levy, M.S., I.J. Collins, S.S. Yim, J.M. Ward, H.N. Titchener-Hooker, P.A. Shamlou, and P. Dunnill. 1999. Effect of shear on plasmid DNA in solution. Bioprocess Eng. 20:7-13. (Pubitemid 29143615)
2. Hirota, S., C.T. de Ilarduya, L.G. Barron, and F.C. Szoka, Jr. 1999. Simple mixing device to reproducibly prepare cationic lipid-DNA complexes (lipoplexes). Biotechniques 27:286-290. (Pubitemid 29382391)
3. Hattori, Y. and M. Hashida. 2005. Evaluation of Size and Zeta Potential of DNA/Carrier Complexes, p. 293-299. Non-viral Gene Therapy. Springer, Tokyo.
4. Gill, D.R., S.E. Smyth, C.A. Goddard, I.A. Pringle, C.F. Higgins, W.H. Colledge, and S.C. Hyde. 2001. Increased persistence of lung gene expression using plasmids containing the ubiquitin C or elongation factor 1alpha promoter. Gene Ther. 8:1539-1546.
5. Eastman, S.J., M.J. Lukason, J.D. Tousignant, H. Murray, M.D. Lane, G.J. St, G.Y. Akita, M. Cherry, et al. 1997. A concentrated and stable aerosol formulation of cationic lipid:DNA complexes giving high-level gene expression in mouse lung. Hum. Gene Ther. 8:765-773.
6. Densmore, C.L., F.M. Orson, B. Xu, B.M. Kinsey, J.C. Waldrep, P. Hua, B. Bhogal, and V. Knight. 2000. Aerosol Delivery of Robust Polyethyleneimine-DNA Complexes for Gene Therapy and Genetic Immunization. Mol. Ther. 1:180-188.
7. Perry, R.H., D.W. Green, and J.O. Maloney. 1986. Perry's Chemical Handbook. McGraw-Hill, New York.
8. Lengsfeld, C.S. and T.J. Anchordoquy. 2002. Shear-induced degradation of plasmid DNA. J. Pharm. Sci. 91:1581-1589.
9. Cupillard, L., V. Juillard, S. Latour, G. Colombet, N. Cachet, S. Richard, S. Blanchard, and L. Fischer. 2005. Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccine to protect cats. Vaccine 23:1910-1916. (Pubitemid 40289061)