Simple mixing device to reproducibly prepare cationic lipid-DNA complexes (lipoplexes)

BioTechniques

Volumn 27, Issue 2, 1999, Pages 286-290

  Hirota, Sadao ^a   De Ilarduya, Conchita Tros ^a   Barron, Lee G ^a   Szoka Jr , Francis C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL; DNA; LIPID; LIPOSOME; PROPANE;

ADOLESCENT; ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; DEVICE; DISPOSABLE EQUIPMENT; DNA BINDING; FEMALE; FLOW RATE; GENE TARGETING; INFUSION PUMP; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PARTICLE SIZE; REPRODUCIBILITY; SHEAR RATE; TECHNIQUE;

EID: 0032810321     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99272bm16     Document Type: Article

References (7)

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2. Bensimon, D., A.J. Simon, V. Croquette and A. Bensimon. 1995. Stretching DNA with a receding meniscus: experiments and models. Phys. Rev. Lett. 74:4754-4757.
3. Bird, R.B., W.E. Stewart and E.N. Lightfoot 1961. Transport Phenomena, p. 208-226. John Wiley & Sons, New York.
4. Gao, X. and L. Huang. 1995. Cationic liposome-mediated gene transfer. Gene Therapy 2:710-722.
5. Meyer, K.B., M.M. Thompson, M.Y. Levy, L.G. Barron and F.C. Szoka, Jr. 1995. Intratracheal gene delivery to the mouse airway: characterization of plasmid DNA expression and pharmacokinetics. Gene Therapy 2:450-460.
6. Perry, R.H., D.W. Green and J.O. Maloney. 1986. Perry's Chemical Handbook, 6th, ed., p. 55-68. MacGraw-Hill, New York.
7. Smyth-Templeton, N., D.D. Lasic, P.M. Frederik, H.H. Strey, D.D. Roberts and G.N. Pavlakis. 1997. Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nature Biotech. 75:647-652.