Expanding the available assays: Adapting and validating in-cell westerns in microfluidic devices for cell-based assays

Assay and Drug Development Technologies

Volumn 8, Issue 5, 2010, Pages 591-601

  Paguirigan, Amy L ^a,b   Puccinelli, John P ^a   Su, Xiaojing ^a   Beebe, David J ^a  

^a University of Wisconsin Health   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSFORMING GROWTH FACTOR BETA;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL ASSAY; CONTROLLED STUDY; EPITHELIAL MESENCHYMAL TRANSITION; GEOMETRY; IMMUNOCYTOCHEMISTRY; INTERMETHOD COMPARISON; LASER; MICROFLUIDICS; MOUSE; NONHUMAN; QUANTITATIVE ANALYSIS; VALIDATION PROCESS;

ANTIBODIES; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL LINE; EPITHELIAL-MESENCHYMAL TRANSITION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOASSAY; MICROFLUIDIC ANALYTICAL TECHNIQUES; REPRODUCIBILITY OF RESULTS; TRANSFORMING GROWTH FACTOR BETA;

EID: 77958548189     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2010.0274     Document Type: Article

References (31)

1. Meyvantsson I, Beebe DJ: Cell culture models in microfluidic systems. Annu Rev Anal Chem 2008;1:423-449.
2. Keenan TM, Folch A: Biomolecular gradients in cell culture systems. Lab Chip 2008;8:34-57.
3. Warrick JW, Murphy WL, Beebe DJ: Screening the cellular microenvironment: a role for microfluidics. Biomed Eng IEEE Rev 2008;1:75-93.
4. Puccinelli JP, Beebe DJ: High throughput micofluidics for biology. In: Microfluidics for Biological Applications (Tian WC, Finehout EJ, eds.,) Springer Science and Business Media, LLC, New York, NY, 2008;241-270.
5. Paguirigan AL, Puccinelli JP, Abhyankar VV, Beebe DJ: Using microfluidics to understand and control the cellular microenvironment. In: Biological Applications of Microfluidics (Gomez FA, ed.,) Wiley-Interscience, Hoboken, NJ, 2007;11-28.
6. El-Ali J, Sorger PK, Jensen KF: Cells on chips. Nature 2006;442:403-411.
7. Dittrich PS, Manz A: Lab-on-a-chip: microfluidics in drug discovery. Nat Rev Drug Discov 2006;5:210-218.
8. Domenech M, Yu H, Warrick J, Badders NM, Meyvantsson I, Alexander CM, Beebe DJ: Cellular observations enabled by microculture: paracrine signaling and population demographics. Integr Biol 2009;1:267-274.
9. Paguirigan AL, Beebe DJ: Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays. Bioessays 2008;30:811-821.
10. Toh YC, Zhang C, Zhang J, Khong YM, Chang S, Samper VD, van Noort D, Hutmacher DW, Yu HR: A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Lab Chip 2007;7:302-309.
11. Workman VL, Dunnett SB, Kille P, Palmer DD: Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells. Biomicrofluidics 2007;1:14105.
12. Jang K, Sato K, Igawa K, Chung UI, Kitamori T: Development of an osteoblast-based 3D continuous-perfusion microfluidic system for drug screening. Anal Bioanal Chem 2008;390:825-832.
13. Kim JY, Park H, Kwon KH, Park JY, Baek JY, Lee TS, Song HR, Park YD, Lee SH: A cell culturing system that integrates the cell loading function on a single platform and evaluation of the pulsatile pumping effect on cells. Biomed Microdevices 2008;10:11-20.
14. Weigum SE, Floriano PN, Christodoulides N, McDevitt JT: Cell-based sensor for analysis of EGFR biomarker expression in oral cancer. Lab Chip 2007;7:995-1003.
15. Karp JM, Yeh J, Eng G, Fukuda J, Blumling J, Suh KY, Cheng J, Mahdavi A, Borenstein J, Langer R, Khademhosseini A: Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells. Lab Chip 2007;7:786-794.
16. Yu H, Alexander CM, Beebe DJ: A plate reader-compatible microchannel array for cell biology assays. Lab Chip 2007;7:388-391.
17. Puccinelli JP, Xiaojing S, Beebe DJ: Automated high-throughput microchannel assays for cell biology: operational optimization and characterization. JALA 2010;15:25-32.
18. Meyvantsson I, Warrick JW, Hayes S, Skoien A, Beebe DJ: Automated cell culture in high density tubeless microfluidic device arrays. Lab Chip 2008;8:717-724.
19. Zhou WH, Du MR, Dong L, Zhu XY, Yang JY, He YY, Li DJ: Cyclosporin A increases expression of matrix metalloproteinase 9 and 2 and invasiveness in vitro of the first-trimester human trophoblast cells via MAPK pathway. Hum Reprod 2007;22:2743-2750.
20. Du Y, Danjo K, Robinson PA, Crabtree JE: In-cell Western analysis of Helicobacter pylori-induced phosphorylation of extracellular-signal related kinase via the transactivation of the epidermal growth factor receptor. Microbes Infect 2007;9:838-846.
21. Honma M, Stubbs M, Collins I, Workman P, Aherne W, Watt FM: Identification of novel keratinocyte differentiation modulating compounds by high-throughput screening. J Biomol Screen 2006;11:977-984.
22. Bhowmick NA, Ghiassi M, Aakre M, Brown K, Singh V, Moses HL: TGF-beta-induced RhoA and p160(ROCK) activation is involved in the inhibition of Cdc25A with resultant cell-cycle arrest. Proc Natl Acad Sci USA 2003;100: 15548-15553.
23. Bhowmick NA, Ghiassi M, Bakin A, Aakre M, Lundquist CA, Engel ME, Arteaga CL, Moses HL: Transforming growth factor-beta 1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol Biol Cell 2001;12:27-36.
24. Zhao BM, Hoffmann FM: Inhibition of transforming growth factor-beta 1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer Trx-SARA. Mol Biol Cell 2006;17:3819-3831.
25. Cui Q, Kyun Lim S, Zhao B, Michael Hoffmann F: Selective inhibition of TGF-[beta] responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP. Oncogene 2005;24:3864-3874.
26. Miettinen PJ, Ebner R, Lopez AR, Derynck R: TGF-beta induced transdifferentiation of mammary epithelial cells to mesenchymal cells: involvement of type I receptors. J Cell Biol 1994;127:2021-2036.
27. Bhowmick N, Ghiassi M, Bakin A, Aakre M, Lundquist C, Engle M, Arteaga C, Moses H: Transforming growth factor-b1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol Biol Cell 2001; 12:27-36.
28. Xia Y, Whitesides GM: Soft lithography. Angew Chem Int Ed 1998;37:550-575.
29. Jo BH, Van Lerberghe LM, Motsegood KM, Beebe DJ: Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer. J Microelectromech Syst 2000;9:76-81.
30. Walker GM, Beebe DJ: A passive pumping method for microfluidic devices. Lab Chip 2002;2:131-134.
31. Paguirigan AL, Beebe DJ: From the cellular perspective: exploring differences in the cellular baseline in macroscale and microfluidic cultures. Integr Biol 2009;1:182-195.