Automated High-Throughput Microchannel Assays for Cell Biology: Operational Optimization and Characterization

Journal of Laboratory Automation

Volumn 15, Issue 1, 2010, Pages 25-32

  Puccinelli, John P ^a   su, Xiaojing ^a   Beebe, David J ^a  

^a University of Wisconsin Health   (United States)

Author keywords

automation; cell culture; immunoassay; liquid handling; microfluidic; passive pumping; protein expression

Indexed keywords

AUTOMATED LIQUID HANDLING; AUTOMATION CELL; CELL BIOLOGY; CELL SEEDING; CELL TYPES; CELL-BASED SCREENS; HIGH THROUGHPUT; HIGH-THROUGHPUT ANALYSIS; IMMUNOCYTOCHEMISTRY; IN-CELL; LIQUID HANDLING; MICROFLUIDIC CHANNEL; OPERATIONAL OPTIMIZATION; PROTEIN EXPRESSIONS; STANDARD DEVIATION; THREE PARAMETERS;

ANTIBODIES; ANTIGENS; AUTOMATION; CELLS; IMMUNOLOGY; LIQUIDS; MICROCHANNELS; MICROFLUIDICS; PUMPS; THROUGHPUT;

CELL CULTURE;

TRANSFORMING GROWTH FACTOR BETA; UVOMORULIN;

ANIMAL CELL; ARTICLE; CELL ASSAY; COCULTURE; CONTROLLED STUDY; HIGH THROUGHPUT SCREENING; IMMUNOCYTOCHEMISTRY; LABORATORY AUTOMATION; LAMINAR FLOW; MICROFLUIDICS; MOUSE; NONHUMAN; PROCESS OPTIMIZATION; RELIABILITY; REPRODUCIBILITY; STAINING;

EID: 72649093318     PISSN: 22110682     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2009.10.002     Document Type: Article

References (18)

1. Meyvantsson I. Warrick J. W. Hayes S. Skoien A. Beebe D. J. Automated cell culture in high density tubeless microfluidic device arrays. Lab Chip 2008, 8(5), 717–724.
2. Paguirigan A.L. Puccinelli J.P. Beebe D.J., Expanding the available assays: adapting and validating In Cell Westerns in microfluidic devices for high throughput cell based assays. In Preparation.
3. Clinton B. M. Lynn R. White E. L. Adapting cell-based assays to the high-throughput screening platform: Problems encountered and lessons learned. JALA 2008, 13(3), 168–173.
4. El-Ali J. Sorger P. K. Jensen K. F. Cells on chips. Nature 2006, 442(7101), 403–411.
5. Kornienko O. Lacson R. Kunapuli P. Schneeweis J. Hoffman I. Smith T. Alberts M. Inglese J. Strulovici B. Miniaturization of whole live cell-based GPCR assays using microdispensing and detection systems. J. Biomol Screen 2004, 9(3), 186–195.
6. Dittrich P. S. Manz A. Lab-on-a-chip: Microfluidics in drug discovery. Nat. Rev. Drug Discov. 2006, 5(3), 210–218.
7. Puccinelli J. P. Beebe D. J. High throughput micofluidics for biology In Microfluidics for Biological Applications; Tian W. C. Finehout E. J., Eds.; Springer Science and Business Media, LLC: New York, NY; 2008; pp 241–270.
8. Walker G. M. Beebe D. J. A passive pumping method for microfluidic devices. Lab Chip 2002, 2(3), 131–134.
9. Berthier E. Beebe D. J. Flow rate analysis of a surface tension driven passive micropump. Lab Chip 2007, 7(11), 1475–1478.
10. Warrick J. Meyvantsson I. Ju J. I. Beebe D. J. High-throughput microfluidics: Improved sample treatment and washing over standard wells. Lab Chip 2007, 7(3), 316–321.
11. Weigl B. H. Morris C. J. Kesler N. Saltsman P. Bardell R. In Well-Plate Formats and Microfluidics—Applications of Laminar Diffusion Interfaces to HTP Screening; Ramsey J. M. van den Berg A., Eds.; Micro Total Analysis Systems: Monterey, CA; 2001; pp 383–384.
12. Yu H. M. Alexander C. M. Beebe D. J. A plate reader-compatible microchannel array for cell biology assays. Lab Chip 2007, 7(3), 388–391.
13. Rossier J. S. Baranek S. Morier P. Vollet C. Vulliet F. De Chastonay Y. Reymond F. GRAVI: Robotized microfluidics for fast and automated immunoassays in low volume. JALA 2008, 13(6), 322–329.
14. Xia Younan Whitesides G. M. Soft lithography. Angew. Chem. Int. Ed. 1998, 37(5), 550–575.
15. Jo B. H. Van Lerberghe L. M. Motsegood K. M. Beebe D. J. Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer. J. Microelectromech. Syst. 2000, 9(1), 76–81.
16. Hancock M. K. Medina M. N. Smith B. M. Orth A. P. Microplate orbital mixing improves high-throughput cell-based reporter assay readouts. J. Biomol. Screen. 2007, 12(1), 140–144.
17. Hannoush R. N. Kinetics of Wnt-driven β-Catenin stabilization revealed by quantitative and temporal imaging. PLoS One 2008, 3(10), e3498.
18. Honma M. Stubbs M. Collins I. Workman P. Aherne W. Watt F. M. Identification of novel keratinocyte differentiation modulating compounds by high-throughput screening. J. Biomol. Screen. 2006, 11(8), 977–984.