Toxicogenomics applied to in vitro carcinogenicity testing with Balb/c 3T3 cells revealed a gene signature predictive of chemical carcinogens

Toxicological Sciences

Volumn 118, Issue 1, 2010, Pages 31-41

  Rohrbeck, Astrid ^a,b   Salinas, Gabriela ^a   Maaser, Kerstin ^a   Linge, Jens ^c   Salovaara, Susan ^c   Corvi, Raffaella ^c   Borlak, Juergen ^a,b  

^a FRAUNHOFER INSTITUTE FOR TOXICOLOGY AND EXPERIMENTAL MEDICINE   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

^c JOINT RESEARCH CENTRE   (Italy)

Author keywords

Balb c 3T3; Cancerogenesis; Cell transformation assay; Microarray; qRT PCR; Toxicogenomic

Indexed keywords

2,4 DIAMINOTOLUENE; 3 METHYLCHOLANTHRENE; ADAMTS1 PROTEIN; ARACHIDONATE 5 LIPOXYGENASE; ASPORIN; BENZO[A]PYRENE; CADHERIN; CADHERIN 13; CARCINOGEN; CD34 ANTIGEN; COLLAGENASE 3; CYTOCHROME P450 1A1; EPIREGULIN; F ACTIN; FIBROMODULIN; GLYPICAN 1; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45; HERMES ANTIGEN; INTERLEUKIN 12 RECEPTOR BETA1; MITOGEN ACTIVATED PROTEIN KINASE KINASE KINASE 4; MITOGEN ACTIVATED PROTEIN KINASE P38; N (2 FLUORENYL)ACETAMIDE; OSTEONECTIN; PHOSPHOLIPASE A2; PROCOLLAGEN; PROSTAGLANDIN SYNTHASE; PROTEIN; SERINE PROTEINASE OMI; STROMELYSIN; TRANSCRIPTION FACTOR RUNX2; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOINFORMATICS; CANCER RISK; CARCINOGENICITY; CELL TRANSFORMATION; CONTROLLED STUDY; CYTOTOXICITY; FIBROBLAST; GENE EXPRESSION; GENETIC ASSOCIATION; GENETIC IDENTIFICATION; GENOMICS; IN VITRO STUDY; MOUSE; NONHUMAN; PREDICTION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TOXICOGENETICS; TOXICOGENOMICS;

2-ACETYLAMINOFLUORENE; ANIMALS; BALB 3T3 CELLS; BENZO(A)PYRENE; CARCINOGENICITY TESTS; CARCINOGENS; CELL SURVIVAL; CELL TRANSFORMATION, NEOPLASTIC; COMPUTATIONAL BIOLOGY; GENE EXPRESSION REGULATION, NEOPLASTIC; METHYLCHOLANTHRENE; MICE; PHENYLENEDIAMINES; PRINCIPAL COMPONENT ANALYSIS; RNA, MESSENGER; TOXICOGENETICS;

HUMAN PAPILLOMAVIRUS;

EID: 77958467782     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfq246     Document Type: Article

References (41)

1. Aikawa, T., Whipple, C. A., Lopez, M. E., Gunn, J., Young, A., Lander, A. D., and Korc, M. (2008). Glypican-1 modulates the angiogenic and metastatic potential of human and mouse cancer cells. J. Clin. Invest. 118, 89-99.
2. Allander, S. V., Illei, P. B., Chen, Y., Antonescu, C. R., Bittner, M., Ladanyi, M., and Meltzer, P. S. (2002). Expression profiling of synovial sarcoma by cDNA microarrays: association of ERBB2, IGFBP2, and ELF3 with epithelial differentiation. Am. J. Pathol. 161, 1587-1595.
3. Bauer, A. K., Fostel, J., Degraff, L. M., Rondini, E. A., Walker, C., Grissom, S. F., Foley, J., and Kleeberger, S. R. (2009). Transcriptomic analysis of pathways regulated by toll-like receptor 4 in a murine model of chronic pulmonary inflammation and carcinogenesis. Mol. Cancer 8, 107.
4. Best, C. J., Gillespie, J. W., Yi, Y., Chandramouli, G. V., Perlmutter, M. A., Gathright, Y., Erickson, H. S., Georgevich, L., Tangrea, M. A., Duray, P. H., et al. (2005). Molecular alterations in primary prostate cancer after androgen ablation therapy. Clin. Cancer Res. 11, 6823-6834.
5. Borlak, J. B. (2005). In Handbook of toxicogenomics: strategies and applications. WILEY-VCH Verlag GmbH & Co, Weinheim, Germany.
6. Bresnick, E., Brosseau, M., Levin, W., Reik, L., Ryan, D. E., and Thomas, P. E. (1981). Administration of 3-methylcholanthrene to rats increases the specific hybridizable mRNA coding for cytochrome p-450c. Proc. Natl. Acad. Sci. U.S.A. 78, 4083-4087.
7. Combes, R., Barratt, M., and Balls, M. (2003). An overall strategy for the testing of chemicals for human hazard and risk assessment under the EU REACH system. Altern. Lab. Anim. 31, 7-19.
8. Daneshmanesh, A. H., Mikaelsson, E., Jeddi-Tehrani, M., Bayat, A. A., Ghods, R., Ostadkarampour, M., Akhondi, M., Lagercrantz, S., Larsson, C., Osterborg, A., et al. (2008). Ror1, a cell surface receptor tyrosine kinase is expressed in chronic lymphocytic leukemia and may serve as a putative target for therapy. Int. J. Cancer 123, 1190-1195.
9. DePierre, J. W., Lundqvist, G., and Ernster, L. (1982). Return of drug-metabolizing systems to control levels after induction with 3-methylcholanthrene. Acta. Chem. Scand. B. 36, 497-498.
10. Ellinger-Ziegelbauer, H., Aubrecht, J., Kleinjans, J. C., and Ahr, H. J. (2009). Application of toxicogenomics to study mechanisms of genotoxicity and carcinogenicity. Toxicol. Lett. 186, 36-44.
11. Esposito, V., Campioni, M., De Luca, A., Spugnini, E. P., Baldi, F., Cassandro, R., Mancini, A., Vincenzi, B., Groeger, A., Caputi, M., et al. (2006). Analysis of HtrA1 serine protease expression in human lung cancer. Anticancer Res. 26, 3455-3459.
12. Fazili, I. S., Jiang, W., Wang, L., Felix, E. A., Khatlani, T., Coumoul, X., Barouki, R., and Moorthy, B. (2010). Persistent induction of cytochrome P450(Cyp)1a1 in human hepatoma cells by 3-methylcholanthrene (MC): Evidence for sustained transcriptional activation of the CYP1A1 promoter. J. Pharmacol. Exp. Ther. 333, 99-109.
13. Guillen-Ahlers, H., Buechler, S. A., Suckow, M. A., Castellino, F. J., and Ploplis, V. A. (2008). Sulindac treatment alters collagen and matrilysin expression in adenomas of ApcMin/+ mice. Carcinogenesis 29, 1421-1427.
14. Hagmar, L., Bonassi, S., Stroemberg, U., Mikoczy, Z., Lando, C., Hansteen, I. L., Montagud, A. H., Knudsen, L., Norppa, H., Reuterwall, C., et al. (1998). Cancer predictive value of cytogenetic markers used in occupational health surveillance programs: a report from an ongoing study by the European Study Group on Cytogenetic Biomarkers and Health. Mutat. Res. 405, 171-178.
15. Jin, Z., Cheng, Y., Olaru, A., Kan, T., Yang, J., Paun, B., Ito, T., Hamilton, J. P., David, S., Agarwal, R., et al. (2008). Promoter hyper-methylation of CDH13 is a common, early event in human esophageal adenocarcinogenesis and correlates with clinical risk factors. Int. J. Cancer 123, 2331-2336.
16. Jones, M. K., Wang, H., Peskar, B. M., Levin, E., Itani, R. M., Sarfeh, I. J., and Tarnawski, A. S. (1999). Inhibition of angiogenesis by nonsteroidal anti-inflammatory drugs: insight into mechanisms and implications for cancer growth and ulcer healing. Nat. Med. 5, 1418-1423.
17. Judson, R. S., Houck, K. A., Kavlock, R. J., Knudsen, T. B., Martin, M. T., Mortensen, H. M., Reif, D. M., Rotroff, D. M., Shah, I., Richard, A. M., et al. (2010). In vitro screening of environmental chemicals for targeted testing priorization: The ToxCast Project. Environ. Health Perspect. 118, 485-492.
18. Kang, G. H., Lee, S., Kim, J. S., and Jung, H. Y. (2003). Profile of aberrant CpG island methylation along multistep gastric carcinogenesis. Lab. Invest. 83, 519-526.
19. Katoh, M., and Katoh, M. (2009). Transcriptional mechanisms of WNT5A based on NF-kappaB, Hedgehog, TGFbeta, and Notch signaling cascades. Int. J. Mol. Med. 23, 763-769.
20. st century: implications for human health risk assessment. Risk. Anal. 49, 5044-5047.
21. Kim, C. J., Yoshioka, N., Tambe, Y., Kushima, R., Okada, Y., and Inoue, H. (2005). Periostin is down-regulated in high grade human bladder cancers and suppresses in vitro cell invasiveness and in vivo metastasis of cancer cells. Int. J. Cancer 117, 51-58.
22. Kirkland, D., Aardema, M., Henderson, L., and Müller, L. (2005). Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens: I. Sensitivity, specificity and relative predictivity. Mutat. Res./Gen. Toxicol. Environ. Mutagen. 584, 1-256.
23. Lekas, P., Tin, K. L., Lee, C., and Prokipcak, R. D. (2000). The human cytochrome P450 1A1 mRNA is rapidly degraded in HepG2 cells. Arch. Biochem. Biophys. 384, 311-318.
24. Lo, H. W., Cao, X., Zhu, H., and Ali-Osman, F. (2010). Cyclooxygenase-2 is a novel transcriptional target of the nuclear EGFR-STAT3 and EGFRvIII-STAT3 signaling axes. Mol. Cancer Res. 8, 232-245.
25. Mathijs, K., Brauers, K. J., Jennen, D. G., Boorsma, A., van Herwijnen, M. H., Gottschalk, R. W., Kleinjans, J. C., and van Delft, J. H. (2009). Discrimination for genotoxic and nongenotoxic carcinogens by gene expression profiling in primary mouse hepatocytes improves with exposure time. Toxicol. Sci. 112, 374-384.
26. Mayr, C., Bund, D., Schlee, M., Moosmann, A., Kofler, D. M., Hallek, M., and Wendtner, C. M. (2005). Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes. Blood 105, 1566-1573.
27. Nebert, D. W., and Dalton, T. P. (2006). The role of cytochrome P450 enzymes in endogenous signalling pathways and environmental carcinogenesis. Nat. Rev. Cancer 6, 947-960.
28. Pasmant, E., Ortonne, N., Rittié, L., Laurendeau, I., Lévy, P., Lazar, V., Parfait, B., Leroy, K., Dessen, P., Valeyrie-Allanore, L., et al. (2010). Differential expression of CCN1/CYR61, CCN3/NOV, CCN4/WISP1, and CCN5/WISP2 in neurofibromatosis type 1 tumorigenesis. J. Neuropathol. Exp. Neurol. 69, 60-69.
29. Peralta-Leal, A., Rodríguez, M. I., and Oliver, F. J. (2008). Poly(ADP-ribose)polymerase-1 (PARP-1) in carcinogenesis: potential role of PARP inhibitors in cancer treatment. Clin. Transl. Oncol. 10, 318-323.
30. Riddick, D. S., Huang, Y., Harper, P. A., and Okey, A. B. (1994). 2,3,7,8-Tetrachlorobenzo-pdioxin versus 3-methylcholanthrene: comparative studies of Ah receptor binding, transformation, and induction of CYP1A1. J. Biol. Chem. 269, 12118-12128.
31. Rohrbeck, A., Müller, V. S., and Borlak, J. (2009). Molecular characterization of lung dysplasia induced by c-Raf-1. PLoS One 4, e5637.
32. Schechtman, L. M. (1985). BALB/c 3T3 cell transformation: protocols, problems and improvements. IARC. Sci. Publ. 67, 165-184.
33. Schröder, C., Schumacher, U., Fogel, M., Feuerhake, F., Müller, V., Wirtz, R. M., Altevogt, P., Krenkel, S., Jänicke, F., and Milde-Langosch, K. (2009). Expression and prognostic value of L1-CAM in breast cancer. Oncol. Rep. 22, 1109-1117.
34. Shamma, A., Yamamoto, H., Doki, Y., Okami, J., Kondo, M., Fujiwara, Y., Yano, M., Inoue, M., Matsuura, N., Shiozaki, H., et al. (2000). Up-regulation of cyclooxygenase-2 in squamous carcinogenesis of the esophagus. Clin. Cancer Res. 6, 1229-1238.
35. Sills, R. C., French, J. E., and Cunningham, M. L. (2001). New models for assessing carcinogenesis: an ongoing process. Toxicol. Lett. 120, 187-198.
36. Tsujii, M., Kawano, S., and Dubois, R. N. (1997). Cyclooxygenase-2 expression in human colon cancer cells increases metastatic potential. Proc. Natl. Acad. Sci. U.S.A. 94, 3336-3340.
37. Turashvili, G., Bouchal, J., Baumforth, K., Wei, W., Dziechciarkova, M., Ehrmann, J., Klein, J., Fridman, E., Skarda, J., Srovnal, J., et al. (2007). Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis. BMC Cancer 27, 7-55.
38. Uehara, T., Hirode, M., Ono, A., Kiyosawa, N., Omura, K., Shimizu, T., Mizukawa, Y., Miyagishima, T., Nagao, T., and Urushidani, T. (2008). A toxicogenomics approach for early assessment of potential non-genotoxic hepatocarcinogenicity of chemicals in rats. Toxicology 250, 15-26.
39. Wong, Y. F., Cheung, T. H., Lo, K. W., Yim, S. F., Siu, N. S., Chan, S. C., Ho, T. W., Wong, K. W., Yu, M. Y., Wang, V. W., et al. (2007). Identification of molecular markers and signaling pathway in endometrial cancer in Hong Kong Chinese women by genome-wide gene expression profiling. Oncogene 26, 1971-1982.
40. Zhan, Q., Lord, K. A., Alamo, I., Hollander, M. C., Carrier, F., Ron, D., Kohn, K. W., Hoffman, B., Liebermann, D. A., and Fornace, A. J. (1994). The gadd and MyD genes define a novel set of mammalian genes encoding acidic proteins that synergistically suppress cell growth. Mol. Cell. Biol. 14, 2361-2371.
41. Zhou, Z. Q., Cao, W. H., Xie, J. J., Lin, J., Shen, Z. Y., Zhang, Q. Y., Shen, J. H., Xu, L. Y., and Li, E. M. (2009). Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinoma. BMC Cancer 9, 291.