Improved antimicrobial activity of h-lysozyme (107-115) by rational Ala substitution

Journal of Peptide Science

Volumn 16, Issue 8, 2010, Pages 424-429

  González, Rodrigo ^a   Albericio, Fernando ^b,c,d   Cascone, Osvaldo ^a   Iannucci, Nancy B ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b INSTITUTE FOR RESEARCH IN BIOMEDICINE   (Spain)

^c BIOMATERIALES Y NANOMEDICINA CIBER BBN   (Spain)

^d UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Alanine scanning; Antimicrobial activity; Lysozyme; Peptides

Indexed keywords

ALANINE; LYSOZYME; LYSOZYME[107-115]; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; PEPTIDE;

AMINO ACID SUBSTITUTION; ANTIMICROBIAL ACTIVITY; ARTICLE; CONTROLLED STUDY; DRUG SCREENING; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; STAPHYLOCOCCUS AUREUS; CHEMISTRY; DRUG EFFECTS; HEMOLYSIS; HUMAN; MICROBIAL SENSITIVITY TEST; STRUCTURE ACTIVITY RELATION; SYNTHESIS;

ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

ALANINE; ANTI-INFECTIVE AGENTS; ESCHERICHIA COLI; HEMOLYSIS; HUMANS; MICROBIAL SENSITIVITY TESTS; MURAMIDASE; PEPTIDES; STAPHYLOCOCCUS AUREUS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 77957292733     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1258     Document Type: Article

References (29)

1. Hancock REW, Diamond G. The role of cationic antimicrobial peptides in innate host defenses. Trends Microbiol. 2000; 8: 402-410.
2. Ganz T. Defensins: antimicrobial peptides of innate immunity. Nat. Rev. Immunol. 2003; 3: 710-720.
3. Rivas-Santiago B, Serrano CJ, Enciso-Moreno JA. Susceptibility to infectious diseases based on antimicrobial peptide production. Infect. Immun. 2009; 77: 4690-4695.
4. Menéndez A, Ferreira RBR, Brett FB. Defensins keep the peace too. Nat. Immunol. 2010; 11: 49-50.
5. Bruckdorfer T, Marder O, Albericio F. From production of peptides in milligram amounts for research tomulti-tons quantities for drugs of the future. Curr. Pharm. Biotechnol. 2004; 5: 29-43.
6. Zompra AZ, Galanis AS, Werbitzky O, Albericio F. Manufacturing peptides as active pharmaceutical ingredients (API). Future Med. Chem. 2009; 1: 361-377.
7. Blondelle SE, Takahashi E, Weber PA, Houghten RA. Identification of antimicrobial peptides by using combinatorial libraries made up of unnatural amino acids. Antimicrob. Agents Chemother. 1994; 38: 2280-2286.
8. Blondelle SE, Pérez-Payá E, Houghten RA. Synthetic combinatorial libraries: novel discovery strategy for identification of antimicrobial agents. Antimicrob. Agents Chemother. 1996; 40: 1067-1071.
9. Pérez-Payá E, Houghten RA, Blondelle SE. Determination of the secondary structure of selected melitin analogues with different hemolytic activities. Biochem. J. 1994; 299: 587-591.
10. Cunningham BC, Wells JA. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science 1989; 244: 1081-1085.
11. Beck-Sickinger AG, Wieland HA, Wittneben H, Willim K-D, Rudolf K, Jung G. Complete L-alanine scan of neuropeptide Y reveals ligands binding to Y1 and Y2 receptors with distinguished conformations. Eur. J. Biochem. 1994; 225: 947-958.
12. Galoppini C, Patacchini R, Meini S, Vigano S, Tancredi M, Quartara L, Triolo A, Maggi CA, Rovero P. A structure-activity study on the bradykinin B1 antagonist desArg10-HOE 140: the alanine scan. Lett. Pept. Sci. 1999; 6: 123-127.
13. Nicole P, Lins L, Rouyer-Fessard C, Drouot C, Fulcrand P, Thomas A, Couvineau A, Martínez J, Brasseur R, Laburthe M. Identification of key residues for interaction of vasoactive intestinal peptide with human VPAC1 and VPAC2 receptors and development of a highly selective VPAC1 receptor agonist. Alanine scanning and molecular modeling of the peptide. J. Biol. Chem. 2000; 275: 24003-24012.
14. Quartara L, Ricci R, Meini S, Patacchini R, Giolitti A, Amadesi S, Rizzi C, Rizzi A, Varani K, Borea PA, Maggi CA, Regoli D. Ala scan analogues of HOE 140. Synthesis and biological activities. Eur. J. Med. Chem. 2000; 35: 1001-1010.
15. Muñoz A, López-García B, Pérez-Payá E, Marcos JF. Antimicrobial properties of derivativesof thecationic tryptophan-rich hexapeptide PAF26. Biochem. Biophys. Res. Commun. 2007; 354: 172-177.
16. Nam J, Shin D, Rew Y, Boger DL. Alanine scan of [L-Dap2]ramoplanin A2 aglycon: assessment of the importance of each residue. J. Am. Chem. Soc. 2007; 129: 8747-8755.
17. Haversen L, Kondori N, Baltzer L, Hanson LA, Dolphin GT, Duner K, Mattsby-Baltzer I. Structure-microbicidal activity relationship of synthetic fragments derived from the antibacterial alpha-helix of human lactoferrin. Antimicrob. Agents Chemother. 2010;54:418-425.
18. Pellegrini A, Thomas U, Bramaz N, Klauser S, Hunziker P, von Fellenberg R. Identification and isolation of a bactericidal domain in chicken egg white lysozyme. J. Appl. Microbiol. 1997; 82: 372-378.
19. Ibrahim HR, Thomas U, Pellegrini A. A helix-loop-helix peptide at the upper lip of the active site cleft of lysozyme confers potent antimicrobial activity with membrane permeabilization action. J. Biol. Chem. 2001; 276: 43767-43774.
20. Mine Y, Ma F, Lauriau S. Antimicrobial peptides released by enzymatic hydrolysis of hen egg white lysozyme. J. Agric. Food Chem. 2004; 52: 1088-1094.
21. Papo N, Shai Y. Can we predict biological activity of antimicrobial peptides from their interactions with model phospholipid membranes? Peptides 2003; 24: 1693-1703.
22. Weinberg A, Krisanaprakornkit S, Dale BA. Epithelial antimicrobial peptides: review and significance for oral applications. Crit. Rev. Oral Biol. Med. 1998; 9: 399-414.
23. Hancock REW, Lehrer R. Cationic peptides: a new source of antibiotics. Trends Biotechnol. 1998; 16: 82-88.
24. Jenssen H, Hamill P, Hancock REW. Peptide antimicrobial agents. Clin.Microbiol. Rev. 2006; 19: 491-511.
25. Papagianni M. Ribosomally synthesized peptides with antimicrobial properties: biosynthesis, structure, function, and applications. Biotechnol. Adv. 2003; 21: 465-499.
26. Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A. Protein identification and analysis tools on the ExPASy server. In The Proteomics Protocols Handbook, Walker JM (eds). Humana Press: Totowa, New Jersey, 2005; 571-607.
27. Guruprasad K, Reddy BVB, Pandit MW. Correlation between stability of a protein and its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its primary sequence. Protein Eng. 1990; 4: 155-161.
28. Ryge TS, Doisy X, Ifrah D, Olsen JE, Hansen PR. New indolicidin analogues with potent antimicrobial activity. J. Pept. Res. 2004; 64: 171-185.
29. Staubitz P, Peschel A, Nieuwenhuizen WF, Otto M, Gotz F, Jung G, Jack RW. Structure-function relationships in the tryptophan-rich, antimicrobial peptide indolicidin. J. Pept. Sci. 2001; 7: 552-564.