Stimulatory effect of insulin on 5α-reductase type 1 (SRD5A1) expression through an Akt-dependent pathway in ovarian granulosa cells

Endocrinology

Volumn 151, Issue 10, 2010, Pages 5030-5037

  Kayampilly, Pradeep P ^a   Wanamaker, Brett L ^a   Stewart, James A ^a   Wagner, Carrie L ^a   Menon, K M J ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INSULIN; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE B; STEROID 5ALPHA REDUCTASE 1; MEMBRANE PROTEIN; ONCOPROTEIN; SRD5A1 PROTEIN, RAT; STEROID 5ALPHA REDUCTASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CATALYSIS; CELL ISOLATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG ACTIVITY; ENZYME INHIBITION; FEMALE; GRANULOSA CELL; HUMAN; HUMAN CELL; INSULIN TREATMENT; NONHUMAN; OVARY; OVULATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; ANIMAL; CELL CULTURE; DOSE RESPONSE; DRUG EFFECTS; ENZYME ACTIVATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGY; SIGNAL TRANSDUCTION; SPRAGUE DAWLEY RAT; UPREGULATION;

3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE; ANIMALS; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME ACTIVATION; FEMALE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GRANULOSA CELLS; HUMANS; INSULIN; MEMBRANE PROTEINS; ONCOGENE PROTEIN V-AKT; OVARY; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; SIGNAL TRANSDUCTION; UP-REGULATION;

EID: 77957264271     PISSN: 00137227     EISSN: 00137227     Source Type: Journal    
DOI: 10.1210/en.2010-0444     Document Type: Article

References (40)

1. Magoffin DA 2006 Ovarian enzyme activities in women with polycystic ovary syndrome. Fertil Steril 86(Suppl 1):S9-S11 (Pubitemid 43913774)
2. Smith OW, Ofner P, Vena RL 1974 In vitro conversion of testosterone-4-14C to androgens of the 5α-androstane series by a normal human ovary. Steroids 24:311-315
3. McNatty KP, Makris A, Reinhold VN, De Grazia C, Osathanondh R, Ryan KJ 1979 Metabolism of androstenedione by human ovarian tissues in vitro with particular reference to reductase and aromatase activity. Steroids 34:429-443
4. Payne DW, Packman JN, Adashi EY 1992 Follicle-stimulating hormone inhibits granulosa cell 5α-reductase activity. Possible role of 5α-reductase as a steroidogenic pubertal switch. J Biol Chem 267:13348-13355
5. Pradeep PK, Li X, Peegel H, Menon KMJ 2002 Dihydrotestosterone inhibits granulosa cell proliferation by decreasing the cyclin D2 mRNA expression and cell cycle arrest at G1 phase. Endocrinology 143:2930-2935 (Pubitemid 34809865)
6. Kayampilly PP, Menon KMJ 2004 Inhibition of extracellular signal-regulated protein kinase-2 phosphorylation by dihydrotestosterone reduces follicle-stimulating hormone-mediated cyclin D2 messenger ribonucleic acid expression in rat granulosa cells. Endocrinology 145:1786-1793
7. Dunaif A, Thomas A 2001 Current concepts in the polycystic ovary syndrome. Annu Rev Med 52:401-419 (Pubitemid 32195332)
8. Nestler JE, Jakubowicz DJ, de Vargas AF, Brik C, Quintero N, Medina F 1998 Insulin stimulates testosterone biosynthesis by human thecal cells from women with polycystic ovary syndrome by activating its own receptor and using inositolglycan mediators as the signal transduction system. J Clin Endocrinol Metab 83:2001-2005 (Pubitemid 28496328)
9. Gilling-Smith C, Willis DS, Beard RW, Franks S 1994 Hypersecretion of androstenedione by isolated thecal cells from polycystic ovaries. J Clin Endocrinol Metab 79:1158-1165 (Pubitemid 24342265)
10. Willis D, Franks S 1995 Insulin action inhumangranulosa cells from normal and polycystic ovaries is mediated by the insulin receptor and not the type-I insulin-like growth factor receptor. J Clin Endocrinol Metab 80:3788-3790
11. Gilling-Smith C, Story H, Rogers V, Franks S 1997 Evidence for a primary abnormality of thecal cell steroidogenesis in the polycystic ovary syndrome. Clin Endocrinol (Oxf) 47:93-99 (Pubitemid 27313994)
12. Nelson VL, Legro RS, Strauss 3rd JF, McAllister JM 1999 Augmented androgen production is a stable steroidogenic phenotype of propagated theca cells from polycystic ovaries. Mol Endocrinol 13:946-957
13. Poretsky L, Smith D, Seibel M, Pazianos A, Moses AC, Flier JS 1984 Specific insulin binding sites in human ovary. J Clin Endocrinol Metab 59:809-811 (Pubitemid 14012513)
14. Wu XK, Zhou SY, Liu JX, Pöllänen P, Sallinen K, Mäkinen M, Erkkola R 2003 Selective ovary resistance to insulin signaling in women with polycystic ovary syndrome. Fertil Steril 80:954-965 (Pubitemid 37230033)
15. Kayampilly PP, Menon KMJ 2006 Dihydrotestosterone inhibits insulin-stimulated cyclin D2 messenger ribonucleic acid expression in rat ovarian granulosa cells by reducing the phosphorylation of insulin receptor substrate-1. Endocrinology 147:464-471
16. Plymate SR, Matej LA, Jones RE, Friedl KE 1988 Inhibition of sex hormone-binding globulin production in thehumanhepatoma (Hep G2) cell line by insulin and prolactin. J Clin Endocrinol Metab 67:460-464
17. Russell DW, Wilson JD 1994 Steroid 5α-reductase: two genes/two enzymes. Annu Rev Biochem 63:25-61
18. Sanders MM, Midgley Jr AR 1982 Rat granulosa cell differentiation: an in vitro model. Endocrinology 111:614-624
19. Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2[-ΔΔC(T)] method. Methods 25:402-408
20. Habib FK, Ross M, Ho CK, Lyons V, Chapman K 2005 Serenoa repens (Permixon) inhibits the 5α-reductase activity of human prostate cancer cell lines without interfering with PSA expression. Int J Cancer 114:190-194
21. Poretsky L, Seto-Young D, Shrestha A, Dhillon S, Mirjany M, Liu HC, Yih MC, Rosenwaks Z 2001 Phosphatidyl-inositol-3 kinase-independent insulin action pathway(s) in the human ovary. J Clin Endocrinol Metab 86:3115-3119
22. Sekar N, Veldhuis JD 2001 Concerted transcriptional activation of the low density lipoprotein receptor gene by insulin and luteinizing hormone in cultured porcine granulosa-luteal cells: possible convergence of protein kinase a, phosphatidylinositol 3-kinase, and mitogen-activated protein kinase signaling pathways. Endocrinology 142:2921-2928
23. Nishi Y, Yanase T, Mu Y, Oba K, Ichino I, Saito M, Nomura M, Mukasa C, Okabe T, Goto K, Takayanagi R, Kashimura Y, Haji M, Nawata H 2001 Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor. Endocrinology 142:437-445
24. Chin D, Shackleton C, Prasad VK, Kohn B, David R, Imperato-McGinley J, Cohen H, McMahon DJ, Oberfield SE 2000 Increased 5α-reductase and normal 11β-hydroxysteroid dehydrogenase metabolism of C19 and C21 steroids in a young population with polycystic ovarian syndrome. J Pediatr Endocrinol Metab 13:253-259
25. Hillier SG, van den Boogaard AM, Reichert Jr LE, van Hall EV 1980 Alterations in granulosa cell aromatase activity accompanying preovulatory follicular development in the rat ovary with evidence that 5α-reduced C19 steroids inhibit the aromatase reaction in vitro. J Endocrinol 84:409-419
26. Smith SS, Ojeda SR 1986 Neonatal release of gonadotropins is essential for development of ovarian follicle-stimulating hormone receptors. Biol Reprod 34:219-227
27. Conway BA, Mahesh VB, Mills TM 1990 Effect of dihydrotestosterone on the growth and function of ovarian follicles in intact immature female rats primed with PMSG. J Reprod Fertil 90:267-277
28. Stewart PM, Shackleton CH, Beastall GH, Edwards CR 1990 5α-Reductase activity in polycystic ovary syndrome. Lancet 335:431-433
29. Agarwal SK, Judd HL, Magoffin DA 1996 A mechanism for the suppression of estrogen production in polycystic ovary syndrome. J Clin Endocrinol Metab 81:3686-3691
30. Fassnacht M, Schlenz N, Schneider SB, Wudy SA, Allolio B, Arlt W 2003 Beyond adrenal and ovarian androgen generation: increased peripheral 5α-reductase activity in women with polycystic ovary syndrome. J Clin Endocrinol Metab 88:2760-2766
31. Kayampilly PP, Menon KMJ 2007 Follicle-stimulating hormone increases tuberin phosphorylation and mammalian target of rapamycin signaling through an extracellular signal-regulated kinase-dependent pathway in rat granulosa cells. Endocrinology 148:3950-3957
32. Haning Jr RV, Tantravahi U, Zhao Q, Hackett RJ, Canick JA 1996 5α-Reductase 1 and 2 expression and activity in human ovarian follicles, stroma and corpus luteum as compared to neonatal foreskin. J Steroid Biochem Mol Biol 59:199-204
33. Mahendroo MS, Cala KM, Russell DW 1996 5α-Reduced androgens play a key role in murine parturition. Mol Endocrinol 10:380-392
34. Mahendroo MS, Russell DW 1999 Male and female isoenzymes of steroid 5α-reductase. Rev Reprod 4:179-183
35. Jakimiuk AJ, Weitsman SR, Magoffin DA 1999 5α-Reductase activity in women with polycystic ovary syndrome. J Clin Endocrinol Metab 84:2414-2418
36. Vassiliadi DA, Barber TM, Hughes BA, McCarthy MI, Wass JA, Franks S, Nightingale P, Tomlinson JW, Arlt W, Stewart PM 2009 Increased 5α-reductase activity and adrenocortical drive in women with polycystic ovary syndrome. J Clin Endocrinol Metab 94:3558-3566
37. Andersson S, Russell DW 1990 Structural and biochemical properties of cloned and expressed human and rat steroid 5α-reductases. Proc Natl Acad Sci USA 87:3640-3644
38. Jenkins EP, Andersson S, Imperato-McGinley J, Wilson JD, Russell DW 1992 Genetic and pharmacological evidence for more than one human steroid 5α-reductase. J Clin Invest 89:293-300
39. Zhang G, Garmey JC, Veldhuis JD 2000 Interactive stimulation by luteinizing hormone and insulin of the steroidogenic acute regulatory (StAR) protein and 17α-hydroxylase/17,20-lyase (CYP17) genes in porcine theca cells. Endocrinology 141:2735-2742
40. Munir I, Yen HW, Geller DH, Torbati D, Bierden RM, Weitsman SR, Agarwal SK, Magoffin DA 2004 Insulin augmentation of 17α-hydroxylase activity is mediated by phosphatidyl inositol 3-kinase but not extracellular signal-regulated kinase-1/2 in human ovarian theca cells. Endocrinology 145:175-183