A 12-week supplementation with quercetin does not affect natural killer cell activity, granulocyte oxidative burst activity or granulocyte phagocytosis in female human subjects

British Journal of Nutrition

Volumn 104, Issue 6, 2010, Pages 849-857

  Heinz, Serena A ^a   Henson, Dru A ^a   Nieman, David C ^a   Austin, Melanie D ^a   Jin, Fuxia ^a  

^a APPALACHIAN STATE UNIVERSITY   (United States)

Author keywords

Immune function; Natural killer cells; Neutrophils; Quercetin

Indexed keywords

INTERLEUKIN 6; PLACEBO; QUERCETIN; TUMOR NECROSIS FACTOR ALPHA; ANTIOXIDANT; PLANT EXTRACT;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; BODY COMPOSITION; BODY FAT; BODY HEIGHT; BODY MASS; BODY WEIGHT; CLINICAL TRIAL; COMMUNITY LIVING; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIET SUPPLEMENTATION; DRUG BLOOD LEVEL; FEMALE; FITNESS; FOOD COMPOSITION; GRANULOCYTE; HUMAN; HUMAN EXPERIMENT; INNATE IMMUNITY; LEUKOCYTE COUNT; LYMPHOCYTE SUBPOPULATION; NATURAL KILLER CELL; NORMAL HUMAN; PHAGOCYTOSIS; RANDOMIZED CONTROLLED TRIAL; RESPIRATORY BURST; SELF REPORT; BLOOD; DOUBLE BLIND PROCEDURE; DRUG EFFECT; IMMUNOLOGY; METABOLISM; MIDDLE AGED; PHYSIOLOGY;

ADULT; AGED; ANTIOXIDANTS; BODY COMPOSITION; BODY MASS INDEX; DIETARY SUPPLEMENTS; DOUBLE-BLIND METHOD; FEMALE; GRANULOCYTES; HUMANS; KILLER CELLS, NATURAL; MIDDLE AGED; PHAGOCYTOSIS; PHYSICAL FITNESS; PLANT EXTRACTS; QUERCETIN; RESPIRATORY BURST;

EID: 77957252267     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S000711451000156X     Document Type: Article

References (48)

1. United States Department of Agriculture (2007) USDA Database for the Flavonoid Content of Selected Foods, Release 2.1. http:// www.ars.usda.gov/ SP2UserFiles/Place/12354500/Data/Flav/Flav 02-1.pdf (accessed 1 October 2009).
2. Manach C, Williamson G & Morand C (2005) Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr 81, Suppl., 230S-242S.
3. Č ́ž M, Pavelková M, Gallová L, et al. (2008) The influence of wine polyphenols on reactive oxygen and nitrogen species production by murine macrophages RAW 264.7. Physiol Res 57, 393-402.
4. Dias AS, Porawski M, Alonso M, et al. (2005) Quercetin decreases oxidative stress, NF-kB activation, and iNOS overexpression in liver of streptozotocin-induced diabetic rats. J. Nutr 135, 2299-2304.
5. Comalada M, Camuesco D, Sierra S, et al. (2005) In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-kB pathway. Eur J Immunol 35, 584-592.
6. Nair MP, Mahajan S, Reynolds JL, et al. (2006) The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor a) gene expression in normal peripheral blood mononuclear cells via modulation of the NF-kB system. Clin Vaccine Immunol 13, 319-328.
7. Vrijsen R, Everaert L & Boeyé A (1988) Antiviral activity of flavones and potentiation by ascorbate. J Gen Virol 69, 1749-1751.
8. Chiang LC, Chiang W, Liu MC, et al. (2003) In vitro antiviral activities of Caesalpinia pulcherrima and its related flavonoids. J Antimicrob Chemother 52, 194-198.
9. Chun OK, Chung SJ & Song WO (2007) Estimated dietary flavonoid intake and major food sources of U.S. adults. J. Nutr 137, 1244-1252.
10. Sampson L, Rimm E, Hollman PCH, et al. (2002) Flavonol and flavone intakes in US health professionals. J Am Diet Assoc 102, 1414-1420.
11. de Boer VCJ, Dihal AA, van der Woude H, et al. (2005) Tissue distribution of quercetin in rats and pigs. J. Nutr 135, 1718-1725.
12. Harwood M, Danielewska-Nikiel B, Borzelleca JF, et al. (2007) A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties. Food Chem Toxicol 45, 2179-2205.
13. Utesch D, Feige K, Dasenbrock J, et al. (2008) Evaluation of the potential in vivo genotoxicity of quercetin. Mutat Res 654, 38-44.
14. Hertog MGL, Feskens EJM, Hollman PCH, et al. (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study. Lancet 342, 1007-1011.
15. Neuhouser ML (2004) Dietary flavonoids and cancer risk: Evidence from human population studies. Nutr Cancer 50, 1-7.
16. Akbay P, Basaran AA, Undeger U, et al. (2003) In vitro immunomodulatory activity of flavonoid glycosides from Urtica dioica L. Phytother Res 17, 34-37.
17. Oršolić N & Bašić I (2005) Water-soluble derivative of propolis and its polyphenolic compounds enhance tumoricidal activity of macrophages. J Ethnopharmacol 102, 37-45.
18. Yu CS, Lai KC & Yang JS (2010) Quercetin inhibited murine leukemia WEHI-3 cells in vivo and promoted immune response. Phytother Res 24, 163-168.
19. Exon JH, Magnuson BA, South EH, et al. (1998) Dietary quercetin, immune functions and colonic carcinogenesis in rats. Immunopharmacol Immunotoxicol 20, 173-190.
20. Bancroft GJ (1993) The role of natural killer cells in innate resistance to infection. Curr Opin Immunol 5, 503-510.
21. Cooper MA, Fehniger TA & Caligiuri MA (2001) The biology of human natural killer-cell subsets. Trends Immunol 22, 633-640.
22. Kobayashi SD, Voyich JM, Burlak C, et al. (2005) Neutrophils in the innate immune response. Arch Immunol Ther Exp 53, 505-517.
23. Henson DA, Nieman DC, Davis JM, et al. (2008) Post-160-km race illness rates and decreases in granulocyte respiratory burst and salivary IgA output are not countered by quercetin ingestion. Int J Sports Med 29, 856-863.
24. Nieman DC, Henson DA, Gross SJ, et al. (2007) Quercetin reduces illness but not immune perturbations after intensive exercise. Med Sci Sports Exerc 39, 1561-1569.
25. Koch A, Kunkel S & Strieter R (1995) Cytokines in rheumatoid arthritis. J Invest Med 43, 28-38.
26. Danesh J, Kaptoge S, Mann AG, et al. (2008) Long-term interleukin-6 levels and subsequent risk of coronary heart disease: Two new prospective studies and a systematic review. PLoS Med 5, e78.
27. Egert S, Bosy-Westphal A, Seiberl J, et al. (2009) Quercetin reduces systolic blood pressure and plasma oxidised lowdensity lipoprotein concentrations in overweight subjects with a high-cardiovascular disease risk phenotype: A doubleblinded, placebo-controlled cross-over study. Br J. Nutr 102, 1065-1074.
28. Davis JM, Murphy EA, Carmichael MD, et al. (2009) Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. Am J Physiol Regul Integr Comp Physiol 296, R1071-R1077.
29. Nieman DC, Henson DA, Davis JM, et al. (2007) Quercetin's influence on exercise-induced changes in plasma cytokines and muscle and leukocyte cytokine mRNA. J Appl Physiol 103, 1728-1735.
30. Nieman DC, Henson DA, Davis JM, et al. (2007) Quercetin ingestion does not alter cytokine changes in athletes competing in the Western States Endurance Run. J Interferon Cytokine Res 27, 1003-1012.
31. Nieman DC, Henson DA, Maxwell KR, et al. (2009) Effects of quercetin and EGCG on mitochondrial biogenesis and immunity. Med Sci Sports Exerc 41, 1467-1475.
32. Chang L, Gusewitch GA, Chritton DBW, et al. (1993) Rapid flow cytometric assay for the assessment of natural killer cell activity. J Immunol Methods 166, 45-54.
33. Bryant J, Day R, Whiteside TL, et al. (1992) Calculation of lytic units for the expression of cell-mediated cytotoxicity. J Immunol Methods 146, 91-103.
34. Perticarari S, Presani G & Banfi E (1994) A new flow cytometric assay for the evaluation of phagocytosis and the oxidative burst in whole blood. J Immunol Methods 170, 117-124.
35. Bastard J-P, Jardel C, Bruckert E, et al. (2000) Elevated levels of interleukin-6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss. J Clin Endocrinol Metab 85, 3338-3342.
36. Rexrode KM, Pradhan A, Manson JE, et al. (2003) Relationship of total and abdominal adiposity with CRP and IL-6 in women. Ann Epidemiol 13, 674-682.
37. Azzolina A (2003) Substance P induces TNF-a and IL-6 production through NFkB in peritoneal mast cells. Biochim Biophys Acta 1643, 75-83.
38. Marquardt D & Walker L (2000) Dependence of mast cell IgEmediated cytokine production on nuclear factor-kB activity. J Allergy Clin Immunol 105, 500-505.
39. Marques-Deak A, Cizza G, Eskandari F, et al. (2006) Measurement of cytokines in sweat patches and plasma in healthy women: Validation in a controlled study. J Immunol Methods 315, 99-109.
40. Nieman DC, Henson DA, Gusewitch G, et al. (1993) Physical activity and immune function in elderly women. Med Sci Sports Exerc 25, 823-831.
41. Nieman DC, Nehlsen-Cannarella SL, Henson DA, et al. (1996) Immune response to obesity and moderate weight loss. Int J Obesity 20, 353-360.
42. Graf BA, Ameho C, Dolnikowski GG, et al. (2006) Rat gastrointestinal tissues metabolize quercetin. J. Nutr 136, 39-44.
43. Nemeth K & Piskula MK (2007) Food content, processing, absorption and metabolism of onion flavonoids. Crit Rev Food Sci Nutr 47, 397-409.
44. Williamson G, Barron D, Shimoi K, et al. (2005) In vitro biological properties of flavonoid conjugates found in vivo. Free Radical Res 39, 457-469.
45. Schoen C, Schulz A, Schweikart J, et al. (2009) Regulatory effects of a fermented food concentrate on immune function parameters in healthy volunteers. Nutrition 25, 499-505.
46. Á lvarez P, Alvarado C, Puerto M, et al. (2006) Improvement of leukocyte functions in prematurely aging mice after five weeks of diet supplementation with polyphenol-rich cereals. Nutrition 22, 913-921.
47. Nieman DC, Buckley KS, Henson DA, et al. (1995) Immune function in marathon runners versus sedentary controls. Med Sci Sports Exerc 27, 986-992.
48. Li Q, Morimoto K, Nakadai A, et al. (2007) Healthy lifestyles are associated with higher levels of perforin, granulysin and granzymes A/B-expressing cells in peripheral blood lymphocytes. Prev Med 44, 117-123.