Protective effect of N-acetyl-l-cysteine against disulfiram-induced oxidative stress and apoptosis in V79 cells

Toxicology and Applied Pharmacology

Volumn 248, Issue 3, 2010, Pages 210-216

  Grosicka Maciag, Emilia ^a   Kurpios Piec, Dagmara ^a   Grzela, Tomasz ^a   Czeczot, Hanna ^a   Skrzycki, Michał ^a   Szumiło, Maria ^a   Rahden Staroń, Iwonna ^a  

^a MEDICAL UNIVERSITY OF WARSAW   (Poland)

Author keywords

Antioxidative enzymes; Apoptosis; Disulfiram; Glutathione; Lipid peroxidation; NAC; Protein carbonyls; V79 cells

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ACETYLCYSTEINE; CARBONYL DERIVATIVE; CATALASE; COPPER ZINC SUPEROXIDE DISMUTASE; DISULFIRAM; GLUTATHIONE; GLUTATHIONE DISULFIDE; GLUTATHIONE PEROXIDASE; MANGANESE SUPEROXIDE DISMUTASE; SELENIUM; THIOBARBITURIC ACID REACTIVE SUBSTANCE; TRYPAN BLUE; ANTIOXIDANT;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL PROTECTION; CELL VIABILITY; CHINESE HAMSTER; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; FIBROBLAST; LIPID PEROXIDATION; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS; PROTEIN CARBONYLATION; STATISTICAL SIGNIFICANCE; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL LINE; COMPARATIVE STUDY; CRICETULUS; DOSE RESPONSE; DRUG EFFECTS; HAMSTER; METABOLISM; PHYSIOLOGY; TOXICITY;

ACETYLCYSTEINE; ANIMALS; ANTIOXIDANTS; APOPTOSIS; CELL LINE; CRICETINAE; CRICETULUS; DISULFIRAM; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBROBLASTS; LIPID PEROXIDATION; OXIDATIVE STRESS;

CRICETULUS GRISEUS;

EID: 77957245225     PISSN: 0041008X     EISSN: 10960333     Source Type: Journal    
DOI: 10.1016/j.taap.2010.08.004     Document Type: Article

References (55)

1. Andrews M., Garle M., Clothier R. Reduction of the new tetrazolium dye in cultured rat hepatocytes and liver functions. ATLA, Altern. Lab. Anim. 1997, 25:641-653.
2. Aruoma O.I., Halliwell B., Hoey B.M., Butler J. The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radical Biol. Med. 1989, 6:593-597.
3. Barth K.S., Malcolm R.J. Disulfiram: an old therapeutic with new applications. CNS Neurol. Disord. Drug Targets 2010, 9:5-12.
4. Beauchamp C., Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 1971, 44:276-287.
5. Blackwell T.S., Blackwell T.R., Holden E.P., Christman B.W., Christman J.W. In vivo antioxidant treatment suppresses nuclear factor-kappa B activation and neutrophilic lung inflammation. J. Immunol. 1996, 157:1630-1637.
6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976, 72:248-254.
7. Bradley M.O., Bhuayan B., Francis M.V., Langenbach R., Peterson A., Huberman E. Mutagenesis by chemical agents in V79 Chinese hamster cells: a review and analysis of the literature. A report of the Gene-Tox Program. Mutat. Res. 1981, 87:81-142.
8. Burkitt M.J., Bishop H.S., Milne L., Tsang S.Y., Provan G.J., Nobel C.S., Orrenius S., Slater A.F. Dithiocarbamate toxicity toward thymocytes involves their copper-catalyzed conversion to thiuram disulfides, which oxidize glutathione in a redox cycle without the release of reactive oxygen species. Arch. Biochem. Biophys. 1998, 353:73-84.
9. Cen D., Bryton D., Shahandeh B., Meyskens F.L., Farmer P.J. Disulfiram facilitates intracellular Cu uptake and induces apoptosis in human melanoma cells. J. Med. Chem. 2004, 47:6914-6920.
10. Chen D., Cui Q.C., Yang H., Dou Q.P. Disulfiram, a clinically used anti-alcoholism drug and copper-binding agent, induces apoptotic cell death in breast cancer cultures and xenografts via inhibition of the proteasome activity. Cancer Res. 2006, 66:10425-10433.
11. Chick J. Safety issues concerning the use of disulfiram in treating alcohol dependence. Drug Saf. 1999, 20:427-435.
12. Circu M.L., Aw T.Y. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radical Biol. Med. 2010, 48:749-762.
13. Darzynkiewicz Z., Juan G., Li X., Gorczyca W., Murakami T., Traganos F. Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 1997, 27:1-20.
14. Delmaestro E., Trombetta L.D. The effects of disulfiram on the hippocampus and cerebellum of the rat brain: a study on oxidative stress. Toxicol. Lett. 1995, 75:235-243.
15. Deneke S.M., Harford P.H., Lee K.Y., Deneke C.F., Wright S.E., Jenkinson S.G. Induction of cysteine transport and other stress proteins by disulfiram: effects on glutathione levels in cultured cells. Am. J. Respir. Cell Mol. Biol. 1997, 17:227-234.
16. Denizot F., Lang R. Rapid colorimetric assay for cell growth and survival, modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 1986, 89:271-277.
17. Dogterom P., Mulder G.J., Nagelkerke J.F. Lipid peroxidation-dependent and -independent protein thiol modifications in isolated rat hepatocytes: differential effects of vitamin E and disulfiram. Chem. Biol. Interact. 1989, 71:291-306.
18. Faiman M.D., Dodd D.E., Hanzlik R.E. Distribution of S35 disulfiram and metabolites in mice, and metabolism of S35 disulfiram in the dog. Res. Commun. Chem. Pathol. Pharmacol. 1978, 21:543-567.
19. Fitsanakis V.A., Amarnat V., Moore J.T., Montine K.S., Zhang J., Montine T.J. Catalysis of catechol oxidation by metal-dithiocarbamate complexes in pesticides. Free Radical Biol. Med. 2002, 33:1714-1723.
20. Forman H.J., York J.L., Fisher A.B. Mechanism for the potentation of oxygen toxicity by disulfiram. J. Pharmacol. Exp. Ther. 1980, 212:452-455.
21. Franco R., Sánchez-Olea R., Reyse-Reyes E.M., Panayiotidis M.I. Environmental toxicity, oxidative stress and apoptosis: ménage à trois. Mutat. Res. 2009, 674:3-22.
22. Goldberg D.M., Spooner R.J. Glutathione reductase. Methods of Enzymatic Analysis 1992, 258-265. Verlag-Chemie. H.U. Bergmayer (Ed.).
23. Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta 1991, 196:143-151.
24. Grosicka E., Sadurska B., Szumiło M., Grzela T., Łazarczyk P., Niderla-Bielińska J., Rahden-Staroń I. Effect of glutathione depletion on apoptosis induced by thiram in Chinese hamster fibroblasts. Int. Immunopharmacol. 2005, 5:1945-1956.
25. Grosicka-Maciag E., Kurpios D., Czeczot H., Szumiło M., Skrzycki M., Suchocki P., Rahden-Staroń I. Changes in antioxidant defense systems induced by thiram in V79 Chinese hamster fibroblasts. Toxicol. in Vitro 2008, 22:28-35.
26. Haddad J.J. Redox and oxidant-mediated regulation of apoptosis signalling pathways: immuno-pharmaco-redox conception of oxidative siege versus cell death commitment. Int. Immunopharmacol. 2004, 4:475-493.
27. Hammond C.L., Lee T.K., Ballatori N. Novel roles of glutathione in gene expression, cell death, and membrane transport of organic solutes. J. Hepatol. 2001, 34:946-954.
28. Hosni M., Meskini N., Prigent A.F., Anker G., Joulain C., El Habib R., Lagarde M. Diethyldithiocarbamate (ditiocarb sodium) effect on arachidonic acid metabolism in human mononuclear cells. Glutathione peroxidase-like activity. Biochem. Pharmacol. 1992, 43:1319-1329.
29. Johansson B. A review of the pharmacokinetics and pharmacodynamics of disulfiram and its metabolites. Acta Psychiatr. Scand. Suppl. 1992, 369:15-26.
30. Krampe H., Ehrenreich H. Supervised Disulfiram as Adjunct to Psychotherapy in Alcoholism Treatment. Curr. Pharm. Des. 2010, 16(19):2076-2090.
31. Kyle M.E., Serroni A., Farber J.L. The inhibition of lipid peroxidation by disulfiram prevents the killing of cultured hepatocytes by allyl alcohol, tert-butyl hydroperoxide, hydrogen peroxide and diethyl maleate. Chem. Biol. Interact. 1989, 72:269-275.
32. Loo T.W., Clarke D.M. Blockage of drug resistance in vitro by Disulfiram, a drug used to treat alcoholism. J. Natl Cancer Inst. 2000, 92:898-902.
33. Maher P. The effects of stress and aging on glutathione metabolism. Ageing Res. Rev. 2005, 4:288-314.
34. Marikovsky M., Ziv V., Nevo N., Harris-Cerruti C., Mahler O. Cu/Zn superoxide dismutase plays important role in immune response. J. Immunol. 2003, 170:2993-3001.
35. Mashiba H., Matsunaga K. Augmented inhibition of tumor cell proliferation in the combined use of disulfiram with ascorbic acid and the involvement of oxygen free radicals. Jpn. J. Exp. Med. 1990, 60:209-214.
36. Miceli M.V., Liles M.R., Newsome D.A. Evaluation of oxidative processes in human pigment epithelial cells associated with retinal outer segment phagocytosis. Exp. Cell Res. 1994, 214:242-249.
37. Michiels C., Raes M., Toussaint O., Remacle J. Importance of Se-glutathione peroxidase, catalase and Cu/Zn-SOD for cell survival against oxidative stress. Free Radical Biol. Med. 1994, 17:235-248.
38. Morrison B.W., Doudican N.A., Patel K.R., Orlow S.J. Disulfiram induces copper-dependent stimulation of reactive oxygen species and activation of the extrinsic apoptotic pathway in melanoma. Melanoma Res. 2010, 20:11-20.
39. Oberley L.W., Spitz D.R. Assay of superoxide dismutase activity in tumor tissue. Methods Enzymol. 1984, 105:457-464.
40. Orrenius S., Nobel C.S., van den Dobbelsteen D.J., Burkitt M.J., Slater A.F. Dithiocarbamates and the redox regulation of cell death. Biochem. Soc. Trans. 1996, 24:1032-1038.
41. Renzi D., Valtonila M., Foster R. The evaluation of a multiendpoint cytotoxicity assay system. ATLA-Alt. Lab. Anim. 1993, 21:89-96.
42. Reznick A.Z., Packer L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol. 1994, 233. 357-263.
43. Sauna Z.E., Peng X.-H., Nandigama K., Tekle S., Ambudkar S.V. The molecular basis of the action of disulfiram as a modulator of the multidrug resistance-linked ATP binding cassette transporters MDR1 (ABCB1) and MRP1 (ABCC1). Mol. Pharmacol. 2004, 675-684.
44. Sauna Z.E., Shukla S., Ambudkar S.V. Disulfiram, an old drug with new potential therapeutic uses for human cancers and fungal infections. Mol. Biosyst. 2005, 1:127-134.
45. Schafer F.Q., Buettner G.R. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radical Biol. Med. 2001, 30:1191-1212.
46. Scozzafava A., Casini A., Supuran C.T. Targeting cysteine residues of biomolecules: new approaches for the design of antiviral and anticancer drugs. Curr. Med. Chem. 2002, 9:1167-1185.
47. Shen M.J., Lipsky J.J., Naylor S. Role of Disulfiram in the in vitro inhibition of rat liver mitochondrial aldehyde dehydrogenase. Biochem. Pharmalol. 2000, 60:947-953.
48. Somers P.K., Medford R.M., Saxena U. Dithiocarbamates: effects on lipid hydroperoxides and vascular inflammatory gene expression. Free Radical Biol. Med. 2000, 28:1532-1537.
49. Suh J.J., Pettinati H.M., Kampman K.M., O'Brien C.P. The status of disulfiram: a half of a century later. J. Clin. Psychopharmacol. 2006, 26:290-302.
50. Townsend D.M., Tew K.D., Tapiero H. The importance of glutathione in human disease. Biomed. Pharmacother. 2003, 57:145-155.
51. Valko M., Rhodes C.J., Moncol J., Izakovic M., Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem. Biol. Interact. 2006, 160:1-40.
52. Wendel A. Glutathione peroxidase. Methods Enzymol. 1981, 77:325-333.
53. Wyllie A.H. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease alternation. Nature 1980, 284:555-556.
54. Yakisich J.S., Siden A., Eneroth P., Cruz M. Disulfiram is a potent in vitro inhibitor of DNA topoisomerases. Biochem. Biophys. Res. Commun. 2001, 289:586-590.
55. Zafarullah M., Li W.Q., Sylvester J., Ahmad M. Molecular mechanisms of N-acetylcysteine actions. Cell. Mol. Life Sci. 2003, 60:6-20.