Detection of campylobacter in gastroenteritis: Comparison of direct PCR assay of faecal samples with selective culture

Epidemiology and Infection

Volumn 121, Issue 3, 1998, Pages 547-553

  Lawson, A J ^a   Shafi, M S ^b   Pathak, K ^b   Stanley, J ^a  

^a CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

^b EALING HOSPITAL NHS TRUST   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; CEFOPERAZONE; CHARCOAL; DEOXYCHOLIC ACID;

ACUTE GASTROENTERITIS; ARTICLE; BACTERIUM CULTURE; CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; COMPARATIVE STUDY; DNA DETERMINATION; FECES; GASTROENTERITIS; HUMAN; HUMAN CELL; NONHUMAN; POLYMERASE CHAIN REACTION;

CAMPYLOBACTER; FECES; GASTROENTERITIS; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0032425911     PISSN: 09502688     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0950268898001630     Document Type: Article

References (15)

1. Skirrow MB. Diseases due to Campylobacter, Helicobacter and related bacteria. J Comp Path 1994; 111: 113-49.
2. Goossens H, Butzler JP. Isolation and identification of Campylobacter spp. In: Nachamkin I, Blaser MJ, Tompkins LS, eds. Campylobacter jejuni: current status and future trends. Washington: American Society for Microbiology, 1992: 93-109.
3. Advisory Committee on the Microbiological Safety of Food. Interim report on campylobacter. London: HMSO, 1993.
4. Linton D, Owen RJ, Stanley J. Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. Res Microbiol 1996; 147: 707-18.
5. Linton D, Lawson AJ, Owen RJ, Stanley J. PCR detection, identification to species level and finger-printing of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. J Clin Microbiol 1997; 35: 2568-72.
6. Boom R, Sol CJA, Salimans MMM, Jansen CL, Wertheim-van Dillen PME, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28: 495-503.
7. Lawson AJ, Linton D, Stanley J, Owen RJ. PCR detection and speciation of Campylobacter upsaliensis and Campylobacter helveticus in human faeces, and comparison with cultural methods. J Appl Microbiol 1997; 83: 375-80.
8. Bolton FJ, Hutchinson DN, Coates D. Blood-free medium for isolation of Campylobacter jejuni from feces. J Clin Microbiol 1984; 19: 169-71.
9. Steele TW, Owen RJ. Campylobacter jejuni subspecies doylei (subsp. nov.), a subspecies of nitrate-negative campylobacters isolated from human clinical specimens. Int J Syst Bacteriol 1988; 38: 316-8.
10. Edmonds P, Patton CM, Griffin PM, et al. Campylobacter hyointestinalis associated with human gastrointestinal disease in the United States. J Clin Microbiol 1987; 25: 685-91.
11. Fennel CL, Rompalo AM, Totten PA, Bruch KL, Flores BM, Stamm WE. Isolation of 'Campylobacter hyointestinalis' from a human. J Clin Microbiol 1986; 24: 146-8.
12. Minet J, Grosbois B, Megraud F. Campylobacter hyointestinalis: an opportunistic enteropathogen? J Clin Microbiol 1988; 26: 2659-60.
13. Mishu B, Patton CM, Tauxe RV. Clinical and epidemiologic features of non-jejuni, non-coli Campylobacter species. In: Nachamkin I, Blaser MJ, Tompkins LS, eds. Campylobacter jejuni: current status and future trends. Washington: American Society for Microbiology, 1992: 31-41.
14. Aspinal ST, Waring DRA, Hayward PG, Hutchinson DN. Selective media for thermophilic campylobacters including C. upsaliensis. J Clin Path 1993; 46: 829-31.
15. Steele TW, McDermott SN. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from faeces. Pathology 1984; 16: 263-5.