A real-time PCR method to quantify spores carrying the Bacillus thuringiensis var. israelensis cry4Aa and cry4Ba genes in soil

Journal of Applied Microbiology

Volumn 109, Issue 4, 2010, Pages 1209-1217

  Guidi, V ^a,b   De Respinis, S ^b   Benagli, C ^b   Luthy P ^c   Tonolla, M ^a,b  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b Cantonal Institute of Microbiology   (Switzerland)

^c ETH ZURICH   (Switzerland)

Author keywords

Bacillus thuringiensis var. israelensis; biocontrol agent; detection; quantification; sediment; soil; TaqMan real time PCR

Indexed keywords

BACTERIOLOGY; BIOGEOCHEMISTRY; DNA; EXTRACTION; GENES; POLYMERASE CHAIN REACTION; SEDIMENTS; SOIL POLLUTION; VALUE ENGINEERING;

BACILLUS THURINGIENSI VAR.; BACILLUS THURINGIENSIS; BIOCONTROL AGENT; DETECTION; ISRAELENSI; ORGANIC MATTER CONTENT; QUANTIFICATION; REAL-TIME PCR; REAL-TIME PCR METHOD; TAQMAN REAL-TIME PCR;

SOILS;

CARBON; ENDOTOXIN; ORGANIC MATTER;

ACCURACY ASSESSMENT; BACTERIUM; BIOCONTROL AGENT; CARBONATE; GENE EXPRESSION; GENETIC ANALYSIS; GRANULOMETRY; HUMIC SUBSTANCE; INHIBITION; INOCULATION; ORGANIC MATTER; PH; PHYSICOCHEMICAL PROPERTY; PLASMID; QUANTITATIVE ANALYSIS; SEDIMENT; SENSITIVITY ANALYSIS; SOIL TYPE; SPECIES DIVERSITY; TOXIN;

ANALYSIS OF VARIANCE; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENE; BACTERIAL SPORE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CRY4AA GENE; CRY4BA GENE; DNA EXTRACTION; NONHUMAN; PLASMID; REAL TIME POLYMERASE CHAIN REACTION; SOIL; SOIL INOCULATION; SOIL PROPERTY;

BACILLUS THURINGIENSIS SEROVAR ISRAELENSIS;

EID: 77956902013     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04741.x     Document Type: Article

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