mRNA extraction and reverse transcription-PCR protocol for detection of nifH gene expression by Azotobacter vinelandii in soil

Applied and Environmental Microbiology

Volumn 69, Issue 4, 2003, Pages 1928-1935

  Bürgmann, Helmut ^a   Widmer, Franco ^b   Sigler, William V ^a   Zeyer, Josef ^a  

^a ETH ZURICH   (Switzerland)

^b Eidg Forschungsanstalt fur Agrarokologie und Landbau   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; EXTRACTION; GENES; NITROGEN FIXATION; OPTIMIZATION; RNA; SOIL TESTING;

GENE EXPRESSION;

MICROBIOLOGY;

AMMONIUM NITRATE; CARBON; DNA; MESSENGER RNA; NITROGEN; SUCROSE;

DETECTION METHOD; GENE EXPRESSION; POLYMERASE CHAIN REACTION; SOIL MICROORGANISM;

ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL GENE; BACTERIAL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; GENE AMPLIFICATION; GENE EXPRESSION; GENE EXPRESSION REGULATION; NIFH GENE; NITROGEN FIXATION; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; SOIL; SOIL MICROFLORA;

AZOTOBACTER VINELANDII; CULTURE MEDIA; GENE EXPRESSION REGULATION, BACTERIAL; NITROGEN FIXATION; OXIDOREDUCTASES; QUATERNARY AMMONIUM COMPOUNDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, MESSENGER; SOIL MICROBIOLOGY;

AZOTOBACTER; AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0242668357     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.4.1928-1935.2003     Document Type: Article

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