A high-throughput method for quantification of glycoprotein sialylation

Analytical Biochemistry

Volumn 407, Issue 1, 2010, Pages 128-133

  Markely, Lam Raga A ^a   Ong, Boon Tee ^b   Hoi, Kong Meng ^b   Teo, Gavin ^b   Lu, Michelle Y ^a   Wang, Daniel I C ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b AGENCY FOR SCIENCE   (Singapore)

Author keywords

Carbohydrate; Glycoprotein; High throughput screening; Mammalian cell culture; Sialic acid

Indexed keywords

ANIMAL CELL CULTURE; BIOACTIVITY; CARBOHYDRATES; GLYCOPROTEINS; GLYCOSYLATION; LIQUID CHROMATOGRAPHY; MAMMALS; PROCESS MONITORING; THROUGHPUT;

CHEMICAL DERIVATIZATION; CHINESE HAMSTER OVARY CELLS; CHROMATOGRAPHIC SEPARATIONS; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT METHOD; MAMMALIAN CELL CULTURE; QUANTITATION LIMITS; SIALIC ACIDS;

CARBOXYLIC ACIDS;

GLYCOPROTEIN; RECOMBINANT GAMMA INTERFERON; SIALIC ACID;

ACCURACY; ANIMAL CELL; ARTICLE; CHO CELL; DERIVATIZATION; ENZYME RELEASE; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; SIALYLATION;

CRICETULUS GRISEUS; MAMMALIA;

EID: 77956886210     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.07.029     Document Type: Article

References (33)

1. Aggarwal S. What's fueling the biotech engine-2008. Nat. Biotechnol. 2009, 27:987-993.
2. Ngantung F.A., Miller P.G., Brushett F.R., Tang G.L., Wang D.I.C. RNA interference of sialidase improves glycoprotein sialic acid content consistency. Biotechnol. Bioeng. 2006, 95:106-119.
3. Kaneko Y., Nimmerjahn F., Ravetch E.V. Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation. Science 2006, 313:670-673.
4. Sinclair A.M., Elliott S. Glycoengineering: the effect of glycosylation on the properties of therapeutic proteins. J. Pharm. Sci. 2005, 94:1626-1635.
5. Goldwasser E., Kung C.K.-H., Eliason J. Mechanism of erythropoietin-induced differentiation: 13. Role of sialic-acid in erythropoietin action. J. Biol. Chem. 1974, 249:4202-4206.
6. Tsuda E., Kawanishi G., Ueda M., Masuda S., Sasaki R. The role of carbohydrate in recombinant human erythropoietin. Eur. J. Biochem. 1990, 188:405-411.
7. Harcum S.W. Protein glycosylation. Cell Culture Technology for Pharmaceutical and Cell-Based Therapies 2006, 113-154. Taylor & Francis, New York. S.S. Ozturk, W.-S. Hu (Eds.).
8. Hossler P., Khattak S.F., Li Z.J. Optimal and consistent protein glycosylation in mammalian cell culture. Glycobiology 2009, 19:936-949.
9. Laughlin S.T., Baskin J.M., Amacher S.L., Bertozzi C.R. In vivo imaging of membrane-associated glycans in developing zebrafish. Science 2008, 320:664-667.
10. Zeng Y., Ramya T.N.C., Dirksen A., Dawson P.E., Paulson J.C. High-efficiency labeling of sialylated glycoproteins on living cells. Nat. Methods 2009, 6:207-209.
11. Baskin J.M., Prescher J.A., Laughlin S.T., Agard N.J., Chang P.V., Miller I.A., Lo A., Codelli J.A., Bertozzit C.R. Copper-free click chemistry for dynamic in vivo imaging. Proc. Natl. Acad. Sci. USA 2007, 104:16793-16797.
12. Gu X.J., Harmon B.J., Wang D.I.C. Site- and branch-specific sialylation of recombinant human interferon-γ in Chinese hamster ovary cell culture. Biotechnol. Bioeng. 1997, 55:390-398.
13. Wong N.S.C., Yap M.G.S., Wang D.I.C. Enhancing recombinant glycoprotein sialylation through CMP-sialic acid transporter over expression in Chinese hamster ovary cells. Biotechnol. Bioeng. 2006, 93:1005-1016.
14. Gawlitzek M., Ryll T., Lofgren J., Sliwkowski M.B. Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms. Biotechnol. Bioeng. 2000, 68:637-646.
15. Gopaul K.P., Crook M.A. Sialic acid: a novel marker of cardiovascular disease?. Clin. Biochem. 2006, 39:667-681.
16. Warren L. Thiobarbituric acid assay of sialic acids. J. Biol. Chem. 1959, 234:1971-1975.
17. Anumula K.R. Rapid quantitative determination of sialic acids in glycoproteins by high-performance liquid chromatography with a sensitive fluorescence detection. Anal. Biochem. 1995, 230:24-30.
18. Honda S., Iwase S., Suzuki S., Kakehi K. Fluorometric determination of sialic acids using malononitrile in weakly alkaline media and its application to postcolumn labeling in high-performance liquid chromatography. Anal. Biochem. 1987, 160:455-461.
19. Li K. Determination of sialic acids in human serum by reversed-phase liquid chromatography with fluorometric detection. J. Chromatogr. 1992, 579:209-213.
20. Knight J.A., Pieper R.K., McClellan L. Specificity of the thiobarbituric acid reaction: its use in studies of lipid peroxidation. Clin. Chem. 1988, 34:2433-2438.
21. Kuwahara S.S. Carbohydrate interference in assays based on the periodate-coupled thiobarbituric acid reagent. Anal. Biochem. 1980, 101:54-60.
22. Love J.C., Ronan J.L., Grotenbreg G.M., van der Veen A.G., Ploegh H.L. A microengraving method for rapid selection of single cells producing antigen-specific antibodies. Nat. Biotechnol. 2006, 24:703-707.
23. Ng S.K., Wang D.I.C., Yap M.G.S. Application of destabilizing sequences on selection marker for improved recombinant protein productivity in CHO-DG44. Metabol. Eng. 2007, 9:304-316.
24. Wong D.C.F., Wong K.T.K., Goh L.T., Heng C.K., Yap M.G.S. Impact of dynamic online fed-batch strategies on metabolism, productivity, and N-glycosylation quality in CHO cell cultures. Biotechnol. Bioeng. 2005, 89:164-177.
25. Hammond K.S., Papermaster D.S. Fluorometric assay of sialic acid in picomole range: modification of thiobarbituric acid assay. Anal. Biochem. 1976, 74:292-297.
26. Gramer M.J., Goochee C.F. Glycosidase activities in Chinese hamster ovary cell lysate and cell-culture supernatant. Biotechnol. Prog. 1993, 9:366-373.
27. Brinkman-Van der Linden E.C.M., Sjoberg E.R., Juneja L.R., Crocker P.R., Varki N., Varki A. Loss of N-glycolylneuraminic acid in human evolution: implications for sialic acid recognition by siglecs. J. Biol. Chem. 2000, 275:8633-8640.
28. Czlapinski J.L., Schelle M.W., Miller L.W., Laughlin S.T., Kohler J.J., Cornish V.W., Bertozzi C.R. Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. J. Am. Chem. Soc. 2008, 130:13186-13187.
29. Hamilton S.R., Davidson R.C., Sethuraman N., Nett J.H., Jiang Y.W., Rios S., Bobrowicz P., Stadheim T.A., Li H.J., Choi B.K., Hopkins D., Wischnewski H., Roser J., Mitchell T., Strawbridge R.R., Hoopes J., Wildt S., Gerngross T.U. Humanization of yeast to produce complex terminally sialylated glycoproteins. Science 2006, 313:1441-1443.
30. Hossler P., Mulukutla B.C., Hu W.-S. Systems analysis of N-glycan processing in mammalian cells. PLoS ONE 2007, 2:e713.
31. Krambeck F.J., Betenbaugh M.J. A mathematical model of N-linked glycosylation. Biotechnol. Bioeng. 2005, 92:711-728.
32. Umana P., Bailey J.E. A mathematical model of N-linked glycoform biosynthesis. Biotechnol. Bioeng. 1997, 55:890-908.
33. Ahn Y.H., Yoo J.S. Malononitrile as a new derivatizing reagent for high-sensitivity analysis of oligosaccharides by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 1998, 12:2011-2015.