Essential modification of the Sircol Collagen assay for the accurate quantification of collagen content in complex protein solutions

Acta Biomaterialia

Volumn 6, Issue 8, 2010, Pages 3146-3151

  Lareu, Ricky R ^a,b,d   Zeugolis, Dimitrios I ^a,b,c   Abu Rub, Mohammad ^c   Pandit, Abhay ^c   Raghunath, Michael ^a,b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b Faculty of Engineering   (Singapore)

^c NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^d UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Collagen content; Hydroxyproline assay; Sircol assay; Sirius red; Ultrafiltration

Indexed keywords

ASSAYS; CELL CULTURE; COLLAGEN; COLORIMETRY; TISSUE;

ACCURATE QUANTIFICATIONS; COLLAGEN CONTENT; CULTURE ENVIRONMENTS; HELICAL MOLECULES; HYDROXYPROLINE ASSAY; NON-COLLAGENOUS PROTEINS; PROTEIN SOLUTION; SIMPLE++; SIRCOL ASSAY; SIRIUS RED;

HYDROXYPROLINE;

COLLAGEN; HYDROXYPROLINE; IMINO ACID; PEPSIN A; PLASMA PROTEIN;

ACCURACY; ARTICLE; BIOMEDICINE; CALORIMETRY; CHEMICAL MODIFICATION; CHROMATOGRAPHY; COLORIMETRY; CULTURE MEDIUM; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; ISOTOPE LABELING; LUNG FIBROBLAST; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PURIFICATION; SIRCOL COLLAGEN ASSAY; TISSUE CULTURE; ULTRAFILTRATION; ANIMAL; BIOASSAY; CELL CULTURE; CYTOLOGY; METABOLISM; METHODOLOGY; SKIN; SOLUTION AND SOLUBILITY; SWINE;

ANIMALS; BIOLOGICAL ASSAY; BLOOD PROTEINS; CELLS, CULTURED; COLLAGEN; HUMANS; PEPSIN A; SKIN; SOLUTIONS; SUS SCROFA; ULTRAFILTRATION;

EID: 77956634669     PISSN: 17427061     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actbio.2010.02.004     Document Type: Article

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