Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate

Applied and Environmental Microbiology

Volumn 76, Issue 17, 2010, Pages 6001-6005

  Tian, Yong Sheng ^a   Xiong, Ai Sheng ^a   Xu, Jing ^a   Zhao, Wei ^a   Gao, Feng ^a   Fu, Xiao Yan ^a   Xu, Hu ^a   Zheng, Jian Li ^a   Peng, Ri He ^a   Yao, Quan Hong ^a  

^a Academy of Agricultural Sciences   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID RESIDUES; CLASS II; GENOMIC LIBRARIES; GLYPHOSATES; OCHROBACTRUM ANTHROPI; SITE DIRECTED MUTAGENESIS; SYNTHASES;

AMINO ACIDS; HERBICIDES; ORGANIC ACIDS;

GENE ENCODING;

3 PHOSPHOSHIKIMATE 1 CARBOXYVINYLTRANSFERASE; BACTERIAL DNA; DRUG DERIVATIVE; ENZYME INHIBITOR; GLYCINE; GLYPHOSATE;

AMINO ACID; BACTERIUM; CLONE; GENE EXPRESSION; GENETIC ANALYSIS; GENOMICS; GLYPHOSATE;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CLUSTER ANALYSIS; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OCHROBACTRUM ANTHROPI; PHYLOGENY; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE; AMINO ACID SEQUENCE; CLONING, MOLECULAR; CLUSTER ANALYSIS; DNA MUTATIONAL ANALYSIS; DNA, BACTERIAL; ENZYME INHIBITORS; GENE EXPRESSION; GLYCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OCHROBACTRUM ANTHROPI; PHYLOGENY; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

OCHROBACTRUM ANTHROPI;

EID: 77956605097     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00770-10     Document Type: Article

References (27)

1. Baerson, S. R., D. J. Rodriguez, M. Tran, Y. Feng, N. A. Biest, and G. M. Dill. 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265-1275.
2. Bentley, R. 1990. The shikimate pathway-a metabolic tree with many branches. Crit. Rev. Biochem. Mol. Biol. 25:307-384.
3. Berti, P. J., and P. Chindemi. 2009. Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis. Biochemistry 48:3699-3707.
4. Castle, L. A., D. L. Siehl, R. Gorton, P. A. Patten, Y. H. Chen, S. Bertain, H.-J. Cho, N. Duck, J. Wong, D. Liu, and M. W. Lassner. 2004. Discovery and directed evolution of a glyphosate tolerance gene. Science 304:1151-1154.
5. Copeland, R. A. 1996. Enzymes: a practical introduction to structure, mechanism, and data analysis. VCH Publishers Inc., New York, NY.
6. Dill, G. M., C. A. Jacob, and S. R. Padgette. 2008. Glyphosate resistant crops: adoption, use and future considerations. Pest Manag. Sci. 64:326-331.
7. Eschenburg, S., M. L. Healy, M. A. Priestman, G. H. Lushington, and E. Schönbrunn. 2002. How the mutation glycine 96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli. Planta 216:129-135.
8. Franz, J. E., M. K. Mao, and J. A. Sikorski. 1997. Glyphosate: a unique global herbicide. Monograph 189. American Chemical Society, Washington, DC.
9. Funke, T., H. Han, M. L. Healy-Fried, M. Fischer, and E. Schönbrunn. 2006. Molecular basis for the herbicide resistance of Roundup Ready crops. Proc. Natl. Acad. Sci. U. S. A. 103:13010-13015.
10. Guex, N., and M. C. Peitsch. 1997. SWISS-MODEL and the Swiss-Pdb-Viewer: an environment for comparative protein modeling. Electrophoresis 18:2714-2723.
11. Haslam, E. 1993. Shikimic acid: metabolism and metabolites, p. 1-6. John Wiley and Sons, New York, NY.
12. He, M., Z. Y. Yang, Y. F. Nie, J. Wang, and P. L. Xu. 2001. A new type of class I bacterial 5-enopyruvylshikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate. Biochim. Biophys. Acta 1568:1-6.
13. He, M., Y. F. Nie, and P. L. Xu. 2003. A T42M substitution in bacterial 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) generates enzymes with increased resistance glyphosate. Biosci. Biotechnol. Biochem. 67:1405-1409.
14. Healy-Fried, M. L., T. Funke, M. A. Priestman, H. Han, and E. Schönbrunn. 2007. Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase. J. Biol. Chem. 282:32949-32955.
15. Kahrizi, D., A. H. Salmanian, A. Afshari, A. Moieni, and A. Mousavi. 2007. Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate. Plant Cell Rep. 26:95-104.
16. Kishore, G. M., and D. M. Shah. 1988. Amino acid biosynthesis inhibitors as herbicides. Annu. Rev. Biochem. 57:627-663.
17. Lanzetta, P. A., L. J. Alvarez, P. S. Reinach, and O. A. Candia. 1979. An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem. 100:95-97.
18. Li, L., W. Lu, Y. Han, S. Ping, W. Zhang, C. Ming, Z. L. Zhao, Y. L. Yan, and L. Min. 2009. A novel RPMXR motif among class II 5-enolpyruvylshikimate-3- phosphate synthases is required for enzymatic activity and glyphosate resistance. J. Biotechnol. 144:330-336.
19. Mizyed, S., J. E. I. Wright, B. Byczynski, and P. J. Berti. 2003. Identification of the catalytic residues of AroA (enolpyruvylshikimate 3-phosphate synthase) using partitioning analysis. Biochemistry 42:6986-6995.
20. Padgette, S. R., D. B. Re, C. S. Gasser, D. A. Eichholtz, R. B. Frazier, C. M. Hironaka, E. B. Levine, D. M. Shah, R. T. Fraley, and G. M. Kishore. 1991. Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate- 3-phosphate synthase active site. J. Biol. Chem. 266:22364-22369.
21. Peng, R. H., A. S. Xiong, and Q. H. Yao. 2006. A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Appl. Microbiol. Biotechnol. 73:234-240.
22. Roberts, F., C. W. Roberts, J. J. Johnson, D. E. Kyle, T. Krell, and J. R. Coggins. 1998. Evidence for the shikimate pathway in apicomplexan parasites. Nature 393:801-805.
23. Schönbrunn, E., S. Eschenburg, W. A. Shuttleworth, J. V. Schloss, N. Amrhein, J. N. Evans, and W. Kabsch. 2001. Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proc. Natl. Acad. Sci. U. S. A. 98:1376-1380.
24. Spencer, M., R. Mumm, and J. Gwyn. March 2000. Glyphosate tolerant maize line. U. S. patent 6, 040, 497.
25. Steinrücken, H. C., and N. Amrhein. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl shikimic acid 3-phosphate synthase. Biochem. Biophys. Res. Commun. 94:1207-1212.
26. Sun, Y.-C., Y.-C. Chen, Z.-X. Tian, F.-M. Li, X.-Y. Wang, J. Zhang, Z.-L. Xiao, M. Lin, N. Gilmartin, D. N. Dowling, and Y.-P. Wang. 2005. Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China. Appl. Environ. Microbiol. 71:4771-4776.
27. Zhou, M., H. Xu, X. Wei, Z. Ye, L. Wei, W. Gong, Y. Wang, and Z. Zhu. 2006. Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy. Plant Physiol. 140:184-195.