Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate

Plant Cell Reports

Volumn 26, Issue 1, 2007, Pages 95-104

  Kahrizi, Danial ^a,c   Salmanian, Ali Hatef ^b   Afshari, Afsoon ^b,d   Moieni, Ahmad ^a   Mousavi, Amir ^b  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^c RAZI UNIVERSITY   (Iran)

^d Khatam University   (Iran)

Author keywords

EPSPS; Glyphosate resistance; Plant transformation; Rapeseed (Brassica napus L.); Site directed mutagenesis

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; ENZYMES; GENES; MUTAGENESIS;

5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE (EPSPS); GLYPHOSATE RESISTANCE; PLANT TRANSFORMATION; RAPESEED (BRASSICA NAPUS L.); SITE-DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

3 PHOSPHOSHIKIMATE 1 CARBOXYVINYLTRANSFERASE; ALANINE; DRUG DERIVATIVE; ESCHERICHIA COLI PROTEIN; GLYCINE; GLYPHOSATE; HERBICIDE; PLANT DNA; THREONINE;

AMINO ACID SUBSTITUTION; ARTICLE; DRUG ANTAGONISM; DRUG EFFECT; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HERBICIDE RESISTANCE; METABOLISM; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; SOUTHERN BLOTTING; TRANSGENIC PLANT; TURNIP;

3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE; ALANINE; AMINO ACID SUBSTITUTION; BLOTTING, SOUTHERN; BRASSICA RAPA; DNA, PLANT; ESCHERICHIA COLI PROTEINS; GLYCINE; HERBICIDE RESISTANCE; HERBICIDES; MUTAGENESIS, SITE-DIRECTED; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; THREONINE; TRANSFORMATION, GENETIC;

AMINO ACIDS; BACTERIA; BIOSYNTHESIS; GENES; MUTAGENS;

AGROBACTERIUM; BACTERIA (MICROORGANISMS); BRASSICA NAPUS; ESCHERICHIA COLI; RHIZOBIUM;

EID: 33845617248     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-006-0208-4     Document Type: Article

References (33)

1. Australia New Zealand Food Authority (ANZFA) (1999) Guidelines for the safety assessment of foods to be included in Standard A18 - Food Produced Using Gene Technology
2. Baerson SR, Rodriguez DJ, Tran M, Feng Y, Biest NA, Dill GM (2002) Glyphosate- resistance goose grass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129:1265-1275
3. Bhalla PL, Smith N (1998) Agrobacterium tumefaciens-mediated transformation of cauliflower, Brassica oleracea var. botrytis. Mol Breed 4:531-541
4. Boocock MR, Coggins JR (1983) Kinetics of 5-Enolpyruvylshikimate-3- phosphate synthase inhibition by glyphosate. FEBS Lett 154(1):127-133
5. Cajacob CA, Feng P, Heck J, Alibhai M, Sammons R, Padgette S (2004) Engineering resistance to herbicides. In: Christou P, Klee H (eds) Handbook of plant biotechnology. Wiley, New York, 353-372
6. Comai LD, Facciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1985) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317:741-744
7. Eichholtz DA, Alan D, Gasser CS, Scott C, Kishore GM, Murthy G (2001) United States Patent No. 6,225,114
8. Franz JE, Mao MK, Sikorski JA (1997) Glyphosate's molecular mode of action. In: Glyphosate: a unique global herbicide. American Chemical Society, Washington, DC, pp 521-642
9. Fry J, Barnason A, Horsch R (1987). Transformation of Brassica napus with Agrobacterium based vectors. Plant Cell Rep 6:321-325
10. Gruys KJ, Sikorski JA (1999) Inhibitors of tryptophan, phenylalanine, and tyrosine biosynthesis as herbicides. In: Singh B (ed) Plant amino acids. Marcel Dekker, New York, pp 357-384
11. Haslam E (1993) Shikimic acid: metabolism and metabolites. Wiley, Chi Chester, UK
12. Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir-and T-region of Agrobacterium tumefaciens Ti-plasmid. Nature 303:179-180
13. Höfgen R, Willmitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16:9877
14. Holt JS, Poweles SB, Holtum AM (1993) Mechanisms and organic aspects of herbicide resistance. Annu Rev Plant Physiol Plant Mol Biol 44:203-229
15. James C (2003) ISAAA Briefs: Preview, Global Status of Commercialized Transgenic Crops, In ISAAA Briefs 30, ISAAA, Ithaca, NY
16. Kishore GM, Brundage L, Kolk K, Padgette SR, Rochester D, Huynh QK, della-Cioppa G (1986) Isolation, purification and characterization of a glyphosate tolerant mutant E. coli EPSP synthase. Proc Fed Am Soc Exp Biol 45:1506
17. Kishore GM, Padgette SR, Fraley RT (1992) History of herbicide-tolerant crops, methods of development and current state of the art-emphasis on Glyphosate tolerance. Weed Technol 6:626-634
18. Kuiper HA, Kleter GA, Noordam MY (2000) Risks of the release of transgenic herbicide-resistant plants with respect to humans, animals, and the environment. Crop Prot 19:773-778
19. Malik J, Barry G, Kishore G (1989) The herbicide glyphosate. Bio-Factors 2:17-25
20. Marzabadi MR, Gruys KJ, Pansegran PD, Walker MC, Yuen HK, Sikorski JA (1996) An EPSP synthase inhibitor joining shikimate-3-phosphate with glyphosate: synthesis and ligand binding studies. Biochemistry 35:4199-4210
21. McDowell LM, Schmidt A, Cohen ER, Studelska DR, Schaefer J (1996) Structural constraints on the ternary complex of 5-enolpyruvylshikimic acid-3-phosphate synthase from rotational-echo double-resonance NMR. J Mol Biol 256:160-171
22. Meilan R, Han KH, Ma C, DiFazio SP, Eaton JA, Hoien EA, Stanton BJ, Crockett RP, Taylor ML, James RR, Skinner JS, Jouanian L, Pilate G, Strauss SH (2002) The CP4 transgene provides high level of tolerance to Roundup® herbicide in field-grown hybrid poplars. Canad J Res 32:967-976
23. Moloney MM, Walker JM, Sharma KK (1989) High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 8:238-242
24. Murray MG, Thampson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res 8:4321-4325
25. Padgette SR, Re DB, Gasser CS, Eichholtz DA, Frazier RB, Hironaka CM, Levine EB, Shah DM, Fraley RT, Kishore GM (1991) Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimic acid-3-phosphate synthase active site. J Biol Chem 266:22361-22369
26. Ream JE, Yuen HK, Frazier RB, Sikorski JA (1992) EPSP synthase: binding studies using isothermal titration microcalorimetry and equilibrium dialysis and their implication for ligand recognition and kinetic mechanism. Biochemistry 31:5528-5534
27. Sambrook J, Russel DW (2001) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press. New York, pp 12.1-12.114
28. Sost D, Amrhein N (1990) Substitution of Gly96 to Ala in the 5-enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae results in a greatly reduced affinity for the herbicide glyphosate. Arc Biochem Biophys 282(2):433-436
29. Sharma KK (1987) Control of organ differentiation from somatic tissues and pollen embryogenesis in anther culture of B. juncea. Ph.D. thesis, Department of Botany, University of Delhi
30. Stalker DM, Hiatt WR, Comai LD (1985) A single amino acid substitution in the enzyme 5-enolpyruvylshikimic acid-3-phosphate synthase confers resistance to herbicide glyphosate. J Biol Chem 260:4724-4728
31. Stam M, Mol JNM, Kooper JM (1997) The silence of genes in transgenic plants. Ann Bot 79:3-12
32. Steinruken HC, Amrhein N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem Biophy Res Commun 94:1207-1212
33. Wang HY, Li YF, Xie LX, Xu P (2003) Expression of bacterial aroA mutant, aroA-M1, encoding 5-enolpyruvylshikimste-3-phosphate synthase for the production of glyphosate-resistant tobacco plants. J Plant Res 116:455-460