A t42m substitution in bacterial 5-enolpyruvylshikimate-3-phosphate synthase (epsps) generates enzymes with increased resistance to glyphosate

Bioscience, Biotechnology and Biochemistry

Volumn 67, Issue 6, 2003, Pages 1405-1409

  He, Ming ^a   Nie, Yan Fang ^a   Xu, Peilin ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

5 enolpyruvylshkimate 3 phosphate synthase (EPSPS); Bacterial aroA; Glyphosate; Herbicide; Mutagenesis

Indexed keywords

3 PHOSPHOSHIKIMATE 1 CARBOXYVINYLTRANSFERASE; DRUG DERIVATIVE; GLYCINE; GLYPHOSATE; HERBICIDE; TRANSFERASE;

AMINO ACID SUBSTITUTION; ARTICLE; DIRECTED MOLECULAR EVOLUTION; DRUG ANTAGONISM; DRUG RESISTANCE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; KINETICS; METABOLISM; MUTATION; SALMONELLA TYPHIMURIUM; TRANSGENIC PLANT;

3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE; ALKYL AND ARYL TRANSFERASES; AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; DRUG RESISTANCE; ESCHERICHIA COLI; GLYCINE; HERBICIDES; KINETICS; MUTATION; PLANTS, GENETICALLY MODIFIED; SALMONELLA TYPHIMURIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA TYPHIMURIUM;

EID: 2142686637     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.67.1405     Document Type: Article

References (15)

1. Schmid, J., and Amrhein, N., Molecular organization of the shikimate pathway in higher plants. Phytochemistry, 39, 737-749 (1995).
2. Herrmann, K. M., and Weaver, L. M., The shikimate pathway. Annu. Rev. Plant Physiol. Plant Mol. Biol., 50, 473-503 (1999).
3. Ream, J. E., Yuen, H. K., Frazier, R. B., and Sikorski, J. A., EPSP syntase: binding studies using isothermal titration microcalorimetry and equilibrium dialysis and their implication for ligand recognition and kinetic mechanism. Biochemistry, 31, 5528-5534 (1992).
4. Marzabadi, M. R., Gruys, K. J., Pansegrau, P. D., Walker, M. C., Yuen, H. K., and Sikorski, J. A., An EPSP synthase inhibitor joining shikimate 3-phosphate with glyphosate: synthesis and ligand binding studies. Biochemistry, 35, 4199-4210 (1996).
5. McDowell, L. M., Schmidt, A., Cohen, E. R., Studelska, D. R., and Schaefer, J., Structural constraints on the ternary complex of 5-enolpyruvylshiki-mate 3-phosphate synthase from rotational-echo double-resonance NMR. J. Mol. Biol., 256, 160-171 (1996).
6. Lewis, J., Johnson, K. A., and Anderson, K. S., The catalytic mechanism of EPSP synthase revisited. Biochemistry, 38, 7372-7379 (1999).
7. Krekel, F., Oecking, C., Amrhein, N., and Macheroux, P., Substrate and inhibitor-induced conformational changes in the structurally related enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS). Biochemistry, 38, 8864-8878 (1999).
8. Stallings, W. C., Abdel-Meguid, S. S., Lim, L. W., Shieh, H., Dayringer, H. E., Leimgruber, N. K., Stegeman, R. A., Anderson, K. S., Sikorski, J. A., Padgette, S. R., and Kishore, G. M., Structure and topological symmetry of the glyphosate target 5-enopyruvylshikimate-3-phosphate synthase: a distinctive protein fold. Proc. Natl. Acad. Sci. USA, 88, 5046-5050 (1991).
9. Schonbrunn, E., Eschenburg, S., Shuttleworth, W. A., Schloss, J. V., Amrhein, N., Evans, J. N. S., and Kabsch, W., Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proc. Natl. Acad. Sci. USA, 98, 1376-1380 (2001).
10. Zhao, H., Giver, L., Shao, Z., Affholter, J. A., and Arnold, F. H., Molecular evolution by staggered extension process (StEP) in vitro recombination. Nature Biotech., 16, 258-261 (1998).
11. He, M., Yang, Z. Y., Nie, Y. F., Wang, J., and Xu, P., A new type of class I bacterial 5-enopyruvyl-shikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate. Biochim. Biophys. Acta, 1568, 1-6 (2001).
12. Knowles, P. F., and Sprinson, D. B., Preparation of shikimate-5-phosphoate. Methods Enzymol., 17A, 351-354 (1997).
13. Lanzetta, P. A., Alvarez, L. J., Reinach, P. S., and Candia, O. A., An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem., 100, 95-97 (1979).
14. Stauffer, M. E., Young, J. K., Helms, G. L., and Evans, J. N. S., Chemical shift mapping of shikimate-3-phosphate binding to the isolated N terminal domain of 5-enolpyruvylshikimate 3-phosphate synthase. FEBS Lett., 499, 182-186 (2001).
15. Shuttleworth, W. A., Pohl, M. E., Helms, G. L., Jakeman, D. L., and Evans, J. N. S., Site-directed mutagenesis of putative active site residues of 5-enol-pyruvylshikimate 3-phosphate synthase. Biochemistry, 38, 296-302 (1999).