Cloning, expression, and hemostatic activities of a disintegrin, r-mojastin 1, from the mohave rattlesnake (Crotalus scutulatus scutulatus)

Thrombosis Research

Volumn 126, Issue 3, 2010, Pages

  Sánchez, Elda E ^a   Lucena, Sara E ^a   Reyes, Steven ^a   Soto, Julio G ^b   Cantu, Esteban ^a   Lopez Johnston, Juan Carlos ^a   Guerrero, Belsy ^c   Salazar, Ana Maria ^c   Rodríguez Acosta, Alexis ^d   Galán, Jacob A ^e   Tao, W Andy ^e   Pérez, John C ^a  

^a TEXAS A AND M UNIVERSITY KINGSVILLE   (United States)

^b SAN JOSE STATE UNIVERSITY   (United States)

^c INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTÍFICAS   (Venezuela)

^d UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

^e PURDUE UNIVERSITY   (United States)

Author keywords

Crotalus scutulatus scutulatus; Hemostasis; Platelet function; r Mojastin 1; Recombinant disintegrins

Indexed keywords

ADENOSINE TRIPHOSPHATE; AMINO ACID; COMPLEMENTARY DNA; CYSTEINE; DISINTEGRIN; FIBRINOGEN; FIBRONECTIN; RECOMBINANT MOJASTIN 1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VITRONECTIN;

ARTICLE; BINDING AFFINITY; CANCER CELL; CROTALUS SCUTULATUS SCUTULATUS; DIALYSIS; DNA SEQUENCE; FREEZE DRYING; HEMOSTASIS; HUMAN; IC 50; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SNAKE; THROMBOCYTE ADHESION; THROMBOCYTE AGGREGATION;

ADENOSINE TRIPHOSPHATE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOOD PLATELETS; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; CROTALID VENOMS; CROTALUS; CYSTEINE; DISINTEGRINS; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBRONECTINS; HEMOSTASIS; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGOPEPTIDES; PLATELET ADHESIVENESS; PLATELET AGGREGATION; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 77956266164     PISSN: 00493848     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.thromres.2010.06.006     Document Type: Article

References (57)

1. J.J. Calvete, M.P. Moreno-Murciano, R.D. Theakston, D.G. Kisiel, and C. Marcinkieicz Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering Biochem J 372 Pt 3 2003 725 734
2. K. Olfa, L. Jose, D. Salma, B. Amine, S.A. Najet, and A. Nicolas Lebestatin, a disintegrin from Macrovipera venom, inhibits integrin-mediated cell adhesion, migration and angiogenesis Lab Investig 5 2005 1507 1516
3. J. Kim, S. Hong, H. Park, D. Kim, and W. Lee Structure and function of RGD peptides derived from disintegrin proteins Mol Cells 19 2005 205 211
4. D. Okuda, H. Koike, and T. Morita A new disintegrin gene structure of the disintegrin family: A subunit of dimeric disintegrin has a short coding region Biochemistry 41 2002 14248 14254
5. Y. Fujii, D. Okuda, Z. Fujimoto, K. Horii, T. Morita, and H. Mizuno Crystal structure of Trimestatin, a disintegrin containing a cell adhesion recognition motif RGD J Mol Biol 332 2003 1115 1122
6. M.A. McLane, E.E. Sánchez, A. Wong, C. Paquette-Straub, and J.C. Pérez Disintegrins Curr Drug Targets-Cardiovas Haemat Dis 4 2004 327 355
7. J.J. Calvete Structure-function correlations of snake venom disintegrins Curr Pharm Des 11 2005 829 835
8. Huang TF, Holt JC, Kirby EP, Niewiarowski S. Trigramin: primary structure and its inhibition of von Willebrand factor binding to glycoprotein IIb/IIIa comlex on human platelets. Biochemistry 1989; 28:661-6.
9. R.M. Kini, and H.J. Evans Structural domains in venom proteins: Evidence that metalloproteinases and nonenzymatic platelet aggregation inhibitors (disintegrins) from snake venoms are derived by proteolysis from a common precursor Toxicon 30 1992 265 293
10. M.C. Corrêa Jr, D.A. Maria, A.M. Moura-da-Silva, K.F. Pizzocaro, and I.R.G. Ruiz Inhibition of melanoma cells tumorigenicity by the snake venom toxin jararhagin Toxicon 40 2002 739 748
11. M.P. Moreno-Murciano, D. Monleón, J.J. Calvete, B. Celda, and C. Marcinkiewicz Amino acid sequence and homology modeling of obtustatin, a novel non-RGD-containing short disintegrin isolated from the venom of Vipera lebetina obtusa Protein Sci 12 2003 366 371
12. E.E. Sánchez, J.A. Galán, W.K. Russell, J.G. Soto, D.H. Russell, and J.C. Pérez Isolation and characterization of two disintegrins inhibitng ADP-induced human platelet aggregation from the venom of Crotalus scutulatus scutulatus (Mohave rattlesnake) Toxicol Appl Pharmacol 212 2006 59 68
13. R. Yang, C. Tang, W. Chuang, T. Huang, H. Peng, and T. Huang Inhibition of tumor formation by snake venom disintegrin Toxicon 45 2005 661 669
14. E.E. Sánchez, A. Rodríguez-Acosta, R. Palomar, S.E. Lucena, S. Bashir, and J.G. Soto Colombistatin: a disintegrin isolated from the venom of the South American snake (Bothrops colombiensis) that effectively inhibits platelet aggregation and SK-Mel-28 cell adhesion Arch Toxicol 83 2009 271 279
15. M.T. Assakura, C.A. Silva, R. Mentele, A.C.M. Camargo, and S.M.T. Serrano Molecular cloning and expression of structural domains of bothropasin, a P-III metalloproteinase from the venom of Bothrops jararaca Toxicon 41 2003 217 227
16. J.H. Fernandez, C.A. Silva, M.T. Assakura, A.C.M. Camargo, and S.M.T. Serrano Molecular cloning, functional expression, and molecular modeling of bothrostatin, a new highly active disintegrin from Bothrops jararaca venom Biochem Biophys Res Commun 329 2005 457 464
17. 1 integrins J Biol Chem 280 2005 40714 40722
18. J.H. Wang, Y. Wu, F. Ren, L. Lu, and B.C. Zhao Cloning and characterization of Adinbitor, a novel disintegrin from the snake venom of Agkistrodon halys brevicaudus stejneger Acta Biochim Biophys Sin 36 2004 425 429
19. Q. Zhou, M.T. Nakada, P.C. Brooks, S.D. Swenson, M.R. Ritter, and S. Argounova Contortrostatin, a homodimeric disintegrin, binds to Integrin αvβ5 Biochem Biophys Res Commun 276 2000 350 355
20. P. Singhamatr, and P. Rojnuckarin Molecular cloning of albolatin, a novel snake venom metalloprotease from green pit viper (Trimeresurus albolabris), and expression of its disintegrin domain Toxicon 50 2007 1192 1200
21. H. Vija, M. Samel, E. Siigur, A. Aaspõllu, K. Tõnismägi, and K. Trummal VGD and MLD-motifs containing heterodimeric disintegrin viplebedin-2 from Vipera lebetina snake venom. Purification and cDNA cloning Comp Biochem Physiol B 153 2009 253 260
22. J.G. Soto, S.A. White, S.R. Reyes, R. Regalado, E.E. Sanchez, and J.C. Perez Molecular Evolution of PIII-SVMP and RGD disintegrin genes from the genus Crotalus Gene 389 2007 66 72
23. J.D. Thompson, and D.G. Higgins and Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple alignment sequence through sequence weighting, positive-specific gap penalties and weight matrix choice Nucleic Acids Res 22 1994 4673 4680
24. S. Subramaniam The Biology Workbench-a seamless database and analysis environment for the biologist Proteins 32 1998 1 2
25. A.M. Salazar, B. Guerrero, B. Cantu, E. Cantu, A. Rodríguez- Acosta, and J.C. Pérez Venom variation in hemostasis of the southern Pacific rattlesnake (Crotalus oreganus helleri): isolation of hellerase Comp Biochem Physiol C Toxicol Pharmacol 149 2009 307 316
26. H. Schägger, and G. von Jagow Tricine-sodium dodeclyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range of 1 to 100 kDa Anal Biochem 166 1987 368 379
27. S. Lucena, A.M. Salazar, A. Gil, C.L. Arocha-Piñango, and B. Guerrero The action of Lonomin V (Lonomia achelous) on fibronectin functional properties Thromb Res 121 2008 653 661
28. M. da Silva, S. Lucena, I. Aguilar, A. Rodríguez-Acosta, A.M. Salazar, and E.E. Sánchez Anti-platelet effect of cumanastatin 1, a disintegrin isolated from venom of South American Crotalus rattlesnake Thromb Res 123 2009 731 739
29. E.E. Sánchez, J.A. Galán, J.C. Perez, A. Rodríguez-Acosta, P.B. Chase, and J.C. Pérez The efficacy of two antivenoms against the venom of North American snakes Toxicon 41 2003 357 365
30. K.J. Clemetson Platelet activation: signal transduction via membrane receptors Thromb Haemost 74 1995 111 116
31. Z.M. Ruggeri, and G.L. Mendolicchio Adhesion mechanisms in platelet function Circ Res 100 2007 1673 1685
32. D.N. Männel, and G.E. Grau Role of platelet adhesion in homeostasis and immunopathology J Clin Pathol Mol Pathol 50 1997 175 185
33. W.P.M. Houdijk, and J.J. Sixma Fibronectin in artery subendothelium is important for platelet adhesion Blood 65 1985 598 604
34. G.D. Laing, and A.M. Moura-da-Silva Jararhagin and its multiple effects on hemostasis Toxicon 45 2005 987 996
35. A.V. Hoffbrand, and J.E. Pettit Platelets in blood coagulation and haemostasis Essential Haematology 3rd ed 1999 Blackwell Science Oxford 209 305
36. B. Hechler, M. Cattaneo, and C. Gachet The P2 Receptors in Platelet Function Semin Thromb Hemost 31 2005 150 161
37. B.N. Kahner, H. Shankar, S. Murugappan, G.L. Prasad, and S.P. Kunapuli Nucleotide receptor signaling in platelets J Thromb Haem 4 2006 2317 2326
38. S.P.M. Crouch, R. Kozlowski, K.J. Slater, and J. Fletcher The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity J Immunol Meth 160 1993 81 88
39. C. Gachet ADP receptors of platelets and their inhibition Thromb Haemost 86 2001 222 232
40. S. Pon, and R. Ponlapat Molecular cloning of albolatin, a novel snake venom metalloprotease from green pit viper (Trimeresurus albolabris), and expression of its disintegrin domain Toxicon 50 2007 1192 1200
41. J.A. Marques, J.K. George, I.J. Smith, V. Bhakta, and W.P. Sheffield A barbourin-albumin fusion protein that is slowly cleared in vivo retains the ability to inhibit platelet aggregation in vitro Thromb Haemost 86 2001 902 908
42. H. Pan, X. Du, G. Yang, Y. Zhou, and X. Wu cDNA cloning and expression of acutin Biochem Biophys Res Commun 255 1999 412 415
43. R.W. Colman, V.J. Marder, A.W. Clowes, J.N. George, and S.Z. Goldhaber Hemostasis and Thrombosis: Basic Principles and Clinical Practices 5th ed 2005 Lippincott Williams &Wilkins
44. N. Wohner Role of cellular elements in thrombus formation and dissolution Cardiovasc Hematol Agents Med Chem 6 2008 224 228
45. M.S. Brower, D.A. Walz, K.E. Garry, and J.W. Fenton 2nd Human neutrophil elastase alters human alpha-thrombin function: limited proteolysis near the gamma-cleavage site results in decreased fibrinogen clotting and platelet-stimulatory activity Blood 69 1987 813 819
46. R. Machovich, and W.G. Owen The elastase-mediated pathway of fibrinolysis Blood Coagul Fibrinolysis 1 1990 79 90
47. W. Schmidt, R. Egbring, and K. Havemann Effect of elastase-like and chymotrypsin-like neutral proteases from human granulocytes on isolated clotting factors Thromb Res 6 1975 315 329
48. P. Henriksson, I.M. Nilsson, K. Ohlsson, and P. Stenberg Granulocyte elastase activation and degradation of factor XIII Thromb Res 18 1980 343 351
49. E. Komorowicz, K. Kolev, I. Léránt, and R. Machovich Flow rate-modulated dissolution of fibrin with clot-embedded and circulating proteases Circ Res 82 1998 1102 1108
50. R.F. Zwaal, and A.J. Schroit Pathophysiologic implications of membrane phospholipids asymmetry in blood cells Blood 89 1997 1121 1132
51. M.E. Carr Jr, and C.L. Hardin Fibrin has larger pores when formed in the presence of erythrocytes Am J Physiol 253 1987 H1069 H1073
52. J.W. Weisel, and R.I. Litvinov The biochemical and physical process of fibrinolysis and effects of clot structure and stability on the lysis rate Cardiovasc Hematol Agents Med Chem 6 2008 161 180
53. M.T. Ganter, and C.K. Hofer Coagulation monitoring: Current techniques and clinical use of viscoelastic point-of-care coagulation devices Int Anesth Res Soc 106 2008 1366 1375
54. I. Wierzbicka-Patynowski, S. Niewiarowski, C. Marcinkiewicz, J.J. Calvete, M.M. Marcinkiewicz, and M.A. McLane Stuctural requirements of echistatin for the recognition of alpha(v)beta(3) and alpha(5)beta(1) integrins J Biol Chem 274 1999 37809 37814
55. M.A. McLane, X. Zhang, J. Tian, C. Zelinskas, A. Srivastava, and B. Hensley Scratching below the surface: Wound healing and alanine mutagenesis provide unique insights into interactions between eristostatin, platelets and melanoma cells Patholphysiol Haemost Thromb 34 2005 164 168
56. D. Park, I. Kang, H. Kim, K. Chung, D.-S. Kim, and Y. Yun Cloning and characterization of novel disintegrins from Agkistrodon halys venom Mol Cells 8 1998 578 584
57. I. Kang, K.H. Chung, S.J. Lee, Y. Yun, H.M. Moon, and D.S. Kim Purification and molecular cloning of a platelet aggregation inhibitor from the snake (Agkistrodon halys brevicaudus) venom Thromb Res 91 1998 65 73