A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids

Biomaterials

Volumn 31, Issue 32, 2010, Pages 8436-8444

  Markovitz Bishitz, Yael ^a   Tauber, Yishay ^a   Afrimzon, Elena ^a   Zurgil, Naomi ^a   Sobolev, Maria ^a   Shafran, Yana ^a   Deutsch, Assaf ^b   Howitz, Steffen ^c   Deutsch, Mordechai ^a  

^a BAR ILAN UNIVERSITY   (Israel)

^b WBT Ltd   (Israel)

^c GeSiM mbH   (Germany)

Author keywords

3D culturing; Microchamber array; Multicellular breast cancer spheroids; Spheroid based drug screening; UV adhesive microstructure

Indexed keywords

3D CULTURING; BREAST CANCER; DRUG SCREENING; MICROCHAMBER ARRAY; UV ADHESIVE MICROSTRUCTURE;

CELL CULTURE; DRUG PRODUCTS; DRUG PRODUCTS PLANTS; MICROCHANNELS; MICROSTRUCTURE; MONOLAYERS; THREE DIMENSIONAL;

SPHERES;

DOXORUBICIN; METHOTREXATE; POLYMER;

ANTINEOPLASTIC ACTIVITY; ARTICLE; BREAST CANCER; CELL CULTURE; CONTROLLED STUDY; CULTURE TECHNIQUE; CULTURE VESSEL; DRUG SCREENING; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SPHEROID CELL;

ANTINEOPLASTIC AGENTS; BREAST NEOPLASMS; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; DRUG SCREENING ASSAYS, ANTITUMOR; EQUIPMENT DESIGN; FEMALE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; POLYMERS; SPHEROIDS, CELLULAR;

EID: 77956188264     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2010.07.050     Document Type: Article

References (32)

1. Allan A.L., Vantyghem S.A., Tuck A.B., Chambers A.F. Tumor dormancy and cancer stem cells: implications for the biology and treatment of breast cancer metastasis. Breast Dis 2006-2007, 26:87-98.
2. Riethdorf S., Pantel K. Disseminated tumor cells in bone marrow and circulating tumor cells in blood of breast cancer patients: current state of detection and characterization. Pathobiology 2008, 75:140-148.
3. Hirschhaeuser F., Menne H., Dittfeld C., West J., Mueller-Klieser W., Kunz-Schughart L.A. Multicellular tumor spheroids: an underestimated tool is catching up again. J Biotechnol 2010.
4. Pampaloni F., Reynaud E.G., Stelzer E.H.K. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 2007, 8:839-845.
5. Justice B.A., Badr N.A., Felder R.A. 3D cell culture opens new dimensions in cell-based assays. Drug Discov Today 2009, 14:102-107.
6. Mazzoleni G., Di Lorenzo D., Steimberg N. Modelling tissues in 3D: the next future of pharmaco-toxicology and food research?. Genes Nutr 2009, 4:13-22.
7. Ghosh S., Spangoli G.C., Martin I., Ploegert S., Demougin P., Herberer M., et al. Three-dimensional culture of melanoma cells profoundly affects gene expression profile: a high density oligonucleotide array study. J Cell Physiol 2005, 204:522-531.
8. Sutherland R.M., Durand R.E. Radiation response of multicell spheroids: an in vitro tumour model. Curr Top Radiat Res Q 1974, 11:87-139.
9. Kunz-Schughart L.A. Multicellular tumor spheroids: intermediates between monolayer culture and in vivo tumor. Cell Biol Int 1999, 23:157-161.
10. Kobayashi H., Man S., Graham C.H., Kapitain S.J., Teicher B.A., Kerbel R.S. Acquired multicellular-mediated resistance to alkylating agents in cancer. Proc Natl Acad Sci U S A 1993, 90:3294-3298.
11. Carlsson J., Yuhas J.M. Liquid-overlay culture of cellular spheroids. Recent Results Cancer Res 1984, 95:1-23.
12. Ivascu A., Kubbies M. Rapid generation of single-tumor spheroids for high-throughput cell function and toxicity analysis. J Biomol Screen 2006, 11:922-932.
13. Kelm J.M., Timmins N.E., Brown C.J., Fussenegger M., Nielsen L.K. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol Bioeng 2003, 83:173-180.
14. Sutherland R.M., McCredie J.A., Inch W.R. Growth of multicell spheroids in tissue culture as a model of nodular carcinomas. J Natl Cancer Inst 1971, 46:113-120.
15. Napolitano A.P., Chai P., Dylan M.D., Jeffrey R.M. Dynamics of the self-assembly of complex cellular aggregates on micromolded nonadhesive hydrogels. Tissue Eng 2007, 13:2087-2094.
16. Sakai Y., Nakazawa K. Technique for the control of spheroid diameter using microfabricated chips. Acta Biomater 2007, 3:1033-1040.
17. Lee J., Cuddihy M.J., Cater G.M., Kotov N.A. Engineering liver tissue spheroids with inverted colloidal crystal scaffolds. Biomaterials 2009, 30:4687-4694.
18. Wu L.Y., Carlo D.D., Lee L.P. Microfluidic self-assembly of tumor spheroids for anticancer drug discovery. Biomed Microdevices 2008, 10:197-202.
19. Zurgil N., Afrimzon E., Deutsch A., Namer Y., Shafran Y., Sobolev M., et al. Polymer live-cell array for real-time kinetic imaging of immune cells. Biomaterials 2010, 31:5022-5029.
20. Durand R.E. Hoechst 33342 for cell selection from multicell systems. J Histochem Cytochem 1982, 30:117-122.
21. Torisawa Y.S., Takagi A., Nashimoto Y., Yasukawa T., Shiku H., Matsue T. A multicellular spheroid array to realize spheroid formation, culture, and viability assay on a chip. Biomaterials 2007, 28:559-566.
22. Friedrich J., Seidel C., Ebner R., Kunz-Schughart L.A. Spheroid-based drug screen: considerations and practical approach. Nat Protoc 2009, 4:309-324.
23. Ponti D., Aurora C., Zaffaroni N., Pratesi G., Petrangolini G., Coradini D., et al. Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Res 2005, 65:5506-5511.
24. Singh S.K., Clarke I.D., Terasaki M., Bonn V.E., Hawkins C., Squire J., et al. Identification of a cancer stem cell in human brain tumors. Cancer Res 2003, 63:5821-5828.
25. Zhang S., Balch C., Chan M.W., Lai H.C., Matei D., Schilder J.M., et al. Identification and characterization of ovarian cancer-initiating cells from primary human tumors. Cancer Res 2008, 68:4311-4320.
26. Fang D., Nguyen T.K., Leishear K., Finko R., Kulp A.N., Hotz S., et al. A tumorigenic subpopulation with stem cell properties in melanomas. Cancer Res 2005, 65:9328-9337.
27. Tang C., Ang B.T., Pervaiz S. Cancer stem cell: target for anti-cancer therapy. FASEB J 2007, 21:3777-3785.
28. Rosen J.M., Jordan C.T. The increasing complexity of the cancer stem cell paradigm. Science 2009, 324:1670-1673.
29. Gupta P.B., Onder T.T., Jiang G., Tao K., Kuperwasser C., Weinberg R.A., et al. Identification of selective inhibitors of cancer stem cells by high-throughput screening. Cell 2009, 138:645-659.
30. + breast cancer-initiating cells to radiation. J Natl Cancer Inst 2006, 98:1777-1785.
31. Cariati M., Naderi A., Brown J.P., Smalley M.J., Pinder S.E., Caldas C., et al. Alpha-6 integrin is necessary for the tumourigenicity of a stem cell-like subpopulation within the MCF7 breast cancer cell line. Int J Cancer 2008, 122:298-304.
32. Zhong Y., Zhou C., Ma W., Wang D., Guo S., Su X., et al. Most MCF7 and SK-OV3 cells were deprived of their stem nature by Hoechst 33342. Biochem Biophys Res Commun 2007, 364:338-343.