Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports

Process Biochemistry

Volumn 45, Issue 10, 2010, Pages 1692-1698

  Bolivar, Juan M ^a   Mateo, Cesar ^a   Grazu, Valeria ^a   Carrascosa, Alfonso V ^b   Pessela, Benevides C ^b   Guisan, Jose M ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

Author keywords

Intramolecular covalent attachment of adsorbed enzymes; Selective immobilization of very large proteins; Stabilization of multimeric enzymes

Indexed keywords

ALKALINITY; CATALYST ACTIVITY; PURIFICATION; STABILIZATION;

ADSORBED ENZYME; AMINO-EPOXY SUPPORTS; COVALENT IMMOBILIZATION; MULTIPOINT COVALENT ATTACHMENTS; ONE-STEP PURIFICATION; PHYSICAL ADSORPTION; SELECTIVE IMMOBILIZATION; STABILIZATION OF MULTIMERIC ENZYMES;

ENZYME IMMOBILIZATION;

THERMUS; THERMUS SP.;

EID: 77956170052     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.07.001     Document Type: Article

References (36)

1. Anchordoquy T.J., Carpenter J.F. Polymers protect lactate dehydrogenase during freeze-drying by inhibiting dissociation in the frozen state. Arch Biochem Biophys 1996, 332(2):231-238.
2. Hudmon A., LeBel E., Roy H., Sik A., Schulman H., Waxham M.N., De Koninck P. A mechanism for Ca2+/calmodulin-dependent protein kinase II clustering at synaptic and nonsynaptic sites based on self-association. J Neurosci 2005, 25(30):6971-6983.
3. Akhtar Md.S., Ahmad A., Bhakuni V. Guanidinium chloride- and urea-induced unfolding of the dimeric enzyme glucose oxidase. Biochemistry 2002, 41(11):3819-3827.
4. Fernandez-Lafuente R. Stabilization of multimeric enzymes: strategies to prevent subunit dissociation. Enzyme Microb Technol 2009, 45(6-7):405-418.
5. Betancor L., Hidalgo A., Fernández-Lorente G., Mateo C., Fernández-Lafuente R., Guisan J.M. Preparation of a stable biocatalyst of bovine liver catalase using immobilization and post-immobilization techniques. Biotechnol Prog 2003, 19(3):763-767.
6. Bolivar J.M., Rocha-Martin J., Mateo C., Cava F., Berenguer J., Fernandez-Lafuente R., Guisan J.M. Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes. Biomacromolecules 2009, 10(4):742-747.
7. Wilson L., Betancor L., Fernández-Lorente G., Fuentes M., Hidalgo A., Guisán J.M., Pessela B.C.C., Fernández-Lafuente R. Cross-linked aggregates of multimeric enzymes: a simple and efficient methodology to stabilize their quaternary structure. Biomacromolecules 2004, 5(3):814-817.
8. Lebbink J.H.G., Knapp S., Van Der Oost J., Rice D., Ladenstein R., De Vos W.M. Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: construction of a 16-residue ion-pair network at the subunit interface. J Mol Biol 1999, 289(2):357-369.
9. Poltorak O.M., Chukhray E.S., Torshin I.Y. Dissociative thermal inactivation, stability, and activity of oligomeric enzymes. Biochemistry (Moscow) 1998, 63(3):303-311.
10. Reznik G.O., Vajda S., Smith C.L., Cantor C.R., Sano T. Streptavidins with intersubunit crosslinks have enhanced stability. Nat Biotechnol 1996, 14(8):1007-1011.
11. Waldburger C.D., Schildbach J.F., Sauer R.T. Are buried salt bridges important for protein stability and conformational specificity?. Nat Struct Biol 1995, 2(2):122-128.
12. Wakarchuk W.W., Sung W.L., Campbell R.L., Cunningham A., Watson D.C., Yaguchi M. Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds. Protein Eng 1994, 7(11):1379-1386.
13. Ahern T.J., Casal J.I., Petsko G.A. Control of oligomeric enzyme thermostability by protein engineering. PNAS 1987, 84(3):675-679.
14. Bolivar J.M., Mateo C., Rocha-Martin J., Cava F., Berenguer J., Fernandez-Lafuente R., Guisan J.M. The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: stabilization of a glutamate dehydrogenase from Thermus thermophilus by immobilization on heterofunctional supports. Enzyme Microb Technol 2009, 44:139-144.
15. Guisán J.M. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb Technol 1988, 10(6):375-382.
16. Armisén P., Mateo C., Cortés E., Barredo J.L., Salto F., Diez B., Rodés L., García J.L., Fernández-Lafuente R., Guisán J.M. Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports. J Chromatogr A 1999, 848(1-2):61-70.
17. Mateo C., Grazu V., Palomo J.M., Lopez-Gallego F., Fernandez-Lafuente R., Guisan J.M. Immobilization of enzymes on heterofunctional epoxy supports. Nat Protoc 2007, 2(5):1022-1033.
18. Pessela B.C.C., Fuentes M., Mateo C., Munilla R., Carrascosa A.V., Fernandez-Lafuente R., Guisan J.M. Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions. Enzyme Microb Technol 2006, 39(4):909-915.
19. Pessela B.C.C., Munilla R., Betancor L., Fuentes M., Carrascosa A.V., Vian A., Fernandez-Lafuente R., Guisán J.M. Ion exchange using poorly activated supports, an easy way for purification of large proteins. J Chromatogr A 2009, 1034:155-159.
20. Zhou Q.Z.K., Chen X.D. Effects of temperature and pH on the catalytic activity of the immobilized β-galactosidase from Kluyveromyces lactis. Biochem Eng J 2001, 9(1):33-40.
21. Ansari S.A., Husain Q. Lactose hydrolysis by β-galactosidase immobilized on concanavalin A-cellulose in batch and continuous mode. J Mol Catal B: Enzym 2010, 63(1-2):68-74.
22. Husain Q. β-galactosidases and their potential applications: a review. Crit Rev Biotechnol 2010, 30(1):41-62.
23. Pessela B.C.C., Mateo C., Fuentes M., Vian A., García J.L., Carrascosa A.V., Guisán J.M., Fernández-Lafuente R. Stabilization of a multimeric β-galactosidase from Thermus sp. strain T2 by immobilization on novel heterofunctional epoxy supports plus aldehyde-dextran cross-linking. Biotechnol Prog 2004, 20:388-392.
24. Snyder S.L., Sobocinski P.Z. An improved 2,4,6 trinitrobenzenesulfonic acid method for the determination of amines. Anal Biochem 1975, 64:284-288.
25. Habeeb A.F.S.A. Determination of free amino groups in proteins by trinitrobenzenesulfonicacid. Anal Biochem 1966, 14:328-336.
26. Fernandez-Lafuente R., Rosell C.M., Rodriguez V., Santana C., Soler G., Bastida A., Guisán J.M. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method. Enzyme Microb Technol 1993, 15:546-550.
27. Grazú V., Abian O., Mateo C., Batista-Viera F., Fernández-Lafuente R., Guisán J.M. Novel bifunctional epoxy/thiol-reactive support to immobilize thiol containing proteins by the epoxy chemistry. Biomacromolecules 2003, 4:1495-1501.
28. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976, 72:248-254.
29. Blanco R.M., Guisan J.M. Stabilization of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivatives. Enzyme Microb Technol 1989, 11(6):360-366.
30. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 27:680-685.
31. Grazú V., Abian O., Mateo C., Batista-Viera F., Fernández-Lafuente R., Guisán J.M. Stabilization of enzymes by multipoint immobilization of thiolated proteins on new epoxy-thiol supports. Biotechnol Bioeng 2005, 90(5):597-605.
32. Pedroche J., Yust M., Mateo C., Fernández-Lafuente R., Girón-Calle J., Alaiz M., Vioque J., Guisán J.M., Millán F. Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: correlation between enzyme-support linkages and thermal stability. Enzyme Microb Technol 2007, 40:1160-1166.
33. Mateo C., Fernández-Lorente G., Abian O., Fernández-Lafuente R., Guisán J.M. Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. Biomacromolecules 2000, 1:739-745.
34. Turkova J., Blaha K., Malanikova M. Methacrylate gels with epoxide groups as supports for immobilization of enzymes in pH range 3-12. Biochim Biophys Acta 1978, 524(1):162-169.
35. Bolivar J.M., Rocha-Martin J., Mateo C., Cava F., Berenguer J., Vega D., Fernandez-Lafuente R., Guisan J.M. Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization. J Mol Catal B: Enzym 2009, 58(1-4):158-163.
36. Bolivar J.M., Rocha-Martin J., Godoy C., Rodrigues R.C., Guisan J.M. Complete reactivation of immobilized derivatives of a trimeric glutamate dehydrogenase from Thermus thermophillus. Process Biochem 2010, 45(1):107-113.