Cross-linked aggregates of multimeric enzymes: A simple and efficient methodology to stabilize their quaternary structure

Biomacromolecules

Volumn 5, Issue 3, 2004, Pages 814-817

  Wilson, Lorena ^a   Betancor, Lorena ^a   Fernández Lorente, Gloria ^a   Fuentes, Manuel ^a   Hidalgo, Aurelio ^a   Guisán, José M ^a   Pessela, Benevides C C ^a   Fernández Lafuente, Roberto ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AGGREGATES; CATALYSIS; CROSSLINKING; DISSOCIATION; ENZYME IMMOBILIZATION;

ENZYME STABILITY; MULTIMERIC ENZYMES;

ENZYMES;

CATALASE; DODECYL SULFATE SODIUM; ENZYME; GLYOXYLIC ACID DERIVATIVE; PROTEIN SUBUNIT; TETRAMER;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DESORPTION; DILUTION; DISSOCIATION; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME STRUCTURE; METHODOLOGY; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN IMMOBILIZATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN STABILITY;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ENZYMES; KINETICS; PROTEIN STRUCTURE, QUATERNARY;

EID: 2542623617     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm034528i     Document Type: Article

References (24)

1. Ghadermarzi, M. Moosavi-Movahedi, A. Influence of different types of effectors on the kinetics parameters of suicide inactivation of catalase by hydrogen peroxide. Biochim. Biophys. Acta 1999, 1431, 30-36.
2. Poltorak, O. M. Chukhray, E. S. Torshin, I. Y. Dissociative thermal inactivation, stability and activity of oligomeric enzymes. Biochem.-Moscow 1998, 63, 360-369.
3. Poltorak, O. M. Chukhray, E. S. Torshin, I. Y. Atyaksheva, L. F. Trevom, M. D. Chaplin, M. F. Catalytic properties, stability and the structure of the conformational lock in the alkaline phosphate from Escherichia coh. J. Mol. Catal. B: Enzym. 1999, 7, 181-192.
4. Attwood, P. V. Geeves, M. A. Changes in catalytic activity and association state of pyruvate carboxylase which are dependent on enzyme concentration. Arch. Biochem. Biophys. 2002, 401, 63-72.
5. Mozhaev, V. V. Klibanov, A. M. Goldmacher, V. S. Berezin, I. V. Operational stability of copolymerized enzymes at elevated temperatures. Biotechnol. Bioeng. 1990, 25, 1937-1945.
6. Fernandez-Lafuente, R. Rodrigues, V. Mateo, C. Penzol, G. Hemandez-Justiz, O. Irazoqui, G. Villariño, A. Ovsejevi, K. Batista, F. Guisan, J. M. Stabilization of multimeric enzymes via immobilization and post-immobilization techniques. J. Mol. Catal. B: Enzym. 1999, 7, 181-189.
7. Fernández-Lafuente, R. Hernández-Ruiz, O. Mateo, C. Terreni, M. Alonso, J. García-López, J. Moreno, M. A. Guisan, J. M. Stabilization of a terrameric enzyme (α-amino acid ester hydrolase from Acetobacter turbidans, enables a very improved performance of ampicillin synthesis. J. Mol. Catal. B: Enzym. 2001, 11, 633-638.
8. Guisán, J. M. Bastida, A. Blanco, R. M. Fernández-Lafuente, R. García-Junceda, E. Immobilization of enzymes by chemical modification with polyfunctional macromolecules. Immobilization of enzymes and cells. In Methods in Biotechnology Bickerstaff, G., Ed. The Humana Press: Totowa, NJ, 1997 pp 261-275.
9. Guisan, J. M. Rodriguez, V. Rosell, C. M. Soler, G. Bastida, A. Blanco, R. M. Fernández-Lafuente, R. García Junceda, E. Stabilization of immobilized enzymes by chemical modification with polyfunctional macromolecules. In Immobilization of enzymes and cells. In Methods in Biotechnology Bickerstaff, G., Ed. The Humana Press: Totowa, NJ, 1997 pp 289-298.
10. Betancor, L. Hidalgo, A. Fernandez-Lorente, G. Mateo, C. Fenandez-Lafuente, R. Guisan, J. M. Preparation of a stable biocatalyst of Bovine Liver catalase using immobilization techniques. Biotechnol. Prog. 2003, 19, 763-767.
11. Cao, L. Van Rantwijk, F. Sheldon, R. Cross-Linked Enzyme Aggregates: A Simple and Effective Method for the Immobilization of Penicillin Acylase. Org. Lett. 2000, 2, 1361-1364.
12. Cao, L. Van Langen, F. Van Rantwijk, F. Sheldon, R. Cross-linked aggregates of penicillin acylase: robust catalysts for the synthesis of B-lactam antibiotics. J. Mol. Catal. B: Enzym. 2001, 11, 665-670.
13. Asano, Y. Overview of screening for new microbial catalysts and their uses in organic synthesis- selection and optimization of biocatalysts. J. Biotechnol. 2002, 94, 65-72.
14. Lozinsky, V. I. Plieva, F. M. Poly (vinyll alcohol) cryogels employed as matrixes for cell immobilization. 3. Overview of recent research and developments. Enzyme Microb. Technol. 1998, 23, 224-242.
15. Noritomi, H. Koyama, K. Kato, S. Nagahama, K. Increased thermostability of cross-linked enzyme crystals of subtilisin in organic solvents. Biotechnol. Technol. 1998, 12, 467-469.
16. van Rantwijk, F. Lau, R. M. Sheldon, R. A. Biocatalytic transformation in ionic liquids. Trends Biotechnol. 2003, 21, 131-138.
17. Guisán, J. M. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb. Technol. 1988, 10, 375-382.
18. 2 solutions in the UV). Anal. Biochem. 1972, 49, 474-478.
19. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
20. Bastida, A. Sabuquillo, P. Armisen, P. Fernández-Lafuente, R. Huguet, J. Guisan, J. M. A single sep purification, immobilization and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic support. Biotechnol. Bioeng. 1998, 58, 486-493.
21. Switala, J. Loewen, P. C. Diversity of properties among catalases. Arch. Biochem. Biphys. 2002, 401, 145-154.
22. Wilson, L. Illanes, A. Pessela, B. C. C Abian, O. Fernández-Lafuente, R. Guisán, J. M. Encapsulation of Cross-Linked Penicillin G Acylase Aggregates in LentiKats. Evaluation of a novel biocatalyst in organic media" Biotechnol. Bioeng. In press.
23. Wilson, L. Illanes, A. Abián, O. Pessela, B. C. C. Fernández-Lafuente, R. Guisán J. M. Co-aggregation of penicillin g acylase and polyionic polymers: a simple methodology to prepare enzyme biocatalysts stable in organic media. Biomacromolecules In press.
24. Hidalgo, A. Betancor, L. López-Gallego F. Moreno, R. Berenguer, J. Fernández-Lafuente, R. Guisán, J. M. " Preparation of a versatile biocatalyst of immobilized and stabilized catalase from Thermus thermophilus". Enzyme Microb. Technol. 2003, 33, 278-285.