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Volumn 132, Issue 34, 2010, Pages 11896-11897

Fluorescent ribonucleoside as a FRET Acceptor for tryptophan in native proteins

Author keywords

[No Author keywords available]

Indexed keywords

NATIVE PROTEINS; RESONANCE ENERGY TRANSFER; REV RESPONSE ELEMENTS; RIBONUCLEOSIDES; RNA BINDING; RNA-BINDING PROTEIN; VISIBLE EMISSIONS;

EID: 77956072696     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja105244t     Document Type: Article
Times cited : (58)

References (28)
  • 3
    • 77956067036 scopus 로고    scopus 로고
    • Trp (1.02% average amino acid composition; ref 1) is 1.74 times more frequently found at RNA binding sites than protein surfaces, while Phe's value is the same. Trp is also 2.56 times more frequently found at RNA binding sites than DNA binding sites (ref 2)
    • Trp (1.02% average amino acid composition; ref 1) is 1.74 times more frequently found at RNA binding sites than protein surfaces, while Phe's value is the same. Trp is also 2.56 times more frequently found at RNA binding sites than DNA binding sites (ref 2).
  • 6
    • 77956073189 scopus 로고    scopus 로고
    • Quinazoline ring substituent type and position influence the emission wavelength of the nucleoside analogue. Substituents in position 7, versus 5, cause a blue shifted emission (ref 7), while reduction of the electron-donating property of the amino substituent also produces a hypsochromic emission shift (ref 8)
    • Quinazoline ring substituent type and position influence the emission wavelength of the nucleoside analogue. Substituents in position 7, versus 5, cause a blue shifted emission (ref 7), while reduction of the electron-donating property of the amino substituent also produces a hypsochromic emission shift (ref 8).
  • 9
    • 77956089112 scopus 로고    scopus 로고
    • See Supporting Information for additional details
    • See Supporting Information for additional details.
  • 21
    • 77956081230 scopus 로고    scopus 로고
    • Fluorescently labeled constructs and displacement assays have been previously used to explore the Rev-RRE system; nearly all have employed large fluorophores. See refs 13, 16, 17, and 19
    • Fluorescently labeled constructs and displacement assays have been previously used to explore the Rev-RRE system; nearly all have employed large fluorophores. See refs 13, 16, 17, and 19.
  • 25
    • 77956072278 scopus 로고    scopus 로고
    • The estimated distance between U66 in RRE and Trp on the Rev peptide is about 20 Å (1ETF)
    • The estimated distance between U66 in RRE and Trp on the Rev peptide is about 20 Å (1ETF).
  • 28
    • 77956084001 scopus 로고    scopus 로고
    • Site-directed mutagenesis can be used to engineer Trp into the RNA binding domain of proteins lacking such residues
    • Site-directed mutagenesis can be used to engineer Trp into the RNA binding domain of proteins lacking such residues.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.