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Volumn 39, Issue 10, 2000, Pages 1788-1790

A novel solid-phase assembly for identifying potent and selective RNA ligands

Author keywords

Analytical methods; Fluorescence spectroscopy; Glycosides; HIV; RNA recognition

Indexed keywords

AMINOGLYCOSIDE ANTIBIOTIC AGENT; FRAMYCETIN; KANAMYCIN A; KANAMYCIN B; LIGAND; REV PROTEIN; RNA BINDING PROTEIN; TOBRAMYCIN;

EID: 0034658578     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-3773(20000515)39:10<1788::AID-ANIE1788>3.0.CO;2-6     Document Type: Article
Times cited : (43)

References (29)
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    • See Supporting Information for experimental details.
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    • [17] In contrast, very low concentrations of the unlabeled Rev peptide or non-biotinylated RRE (40 and 90 nM, respectively) are needed to displace 50% of Rev-Fl. These results taken together suggest a high degree of binding specificity for the immobilixed Rev-RRE complex.
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    • -1 gel), which suggests that the immobilized RRE complexes are noninteracting over this loading range.
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    • 50 values are reported.
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    • -1 of acomplex mixture of yeast pre-tRNAs and mature tRNAs (Sigma type X). This is approximately a 50-fold molar excess of DNA nucleotides and a 200-fold molar excess of a tRNA nucleotides (relative to the RRE). These concentrations lead to a minimal (5- 10 %) displacement of the Rev-Fl from the solid support.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.