Engineering of N. benthamiana L. plants for production of N-acetylgalactosamine-glycosylated proteins - towards development of a plant-based platform for production of protein therapeutics with mucin type O-glycosylation

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Daskalova, Sasha M ^a   Radder, Josiah E ^a   Cichacz, Zbigniew A ^a   Olsen, Sam H ^a   Tsaprailis, George ^b   Mason, Hugh ^a   Lopez, Linda C ^a  

^a ARIZONA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

A PLANTS; BIOLOGICAL FUNCTIONS; DISTRIBUTION PATTERNS; E. COLI; ECTOPIC EXPRESSIONS; ELEGANS; ENDOCHITINASE; ENDOGENOUS ENZYME; ENTEROTOXIN; GLYCOSYLATED; IN-VITRO; ISOFORMS; MAGNICON; MAMMALIAN PROTEINS; O-GLYCOSYLATION; PEPTIDE FUSION; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN THERAPEUTICS; RECOMBINANT ENZYMES; RECOMBINANT GLYCOPROTEINS; TANDEM REPEATS; THREONINE RESIDUES; TOBACCO CELLS; TRANSPORTER GENES;

AMINO ACIDS; BIOREACTORS; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION; GENETIC ENGINEERING; GLYCOSYLATION; INTERNET PROTOCOLS; LAKES; MACHINERY; MAMMALS; PEPTIDES; PHOSPHATASES; POLYPEPTIDES;

ESTERIFICATION;

BREFELDIN A; CHORIONIC GONADOTROPIN BETA SUBUNIT; MUCIN; N ACETYLGALACTOSAMINE; RECOMBINANT ENZYME; VIRUS VECTOR; EPIMERASE; HYBRID PROTEIN; MUCIN 1; N ACETYLGALACTOSAMINYLTRANSFERASE; PLANT RNA; POLYPEPTIDE N ACETYLGALACTOSAMINYLTRANSFERASE; POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE; TN ANTIGEN; TUMOR ANTIGEN; UDP-N-ACETYLGLUCOSAMINE 4-EPIMERASE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE 4 EPIMERASE;

AMINO ACID SEQUENCE; ARTICLE; BIOREACTOR; COMPUTER MODEL; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; GOLGI COMPLEX; LIQUID CHROMATOGRAPHY; NICOTIANA BENTHAMIANA; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; TANDEM MASS SPECTROMETRY; TANDEM REPEAT; TRANSGENIC PLANT; YERSINIA ENTEROCOLITICA; BIOSYNTHESIS; CHEMISTRY; GENETIC TRANSFORMATION; GENETICS; GLYCOSYLATION; HUMAN; METABOLISM; METHODOLOGY; PROTEIN PROCESSING; TOBACCO;

CAENORHABDITIS ELEGANS; HOMO SAPIENS; MAMMALIA; NICOTIANA TABACUM; YERSINIA ENTEROCOLITICA;

ANTIGENS, TUMOR-ASSOCIATED, CARBOHYDRATE; CARBOHYDRATE EPIMERASES; GENETIC ENGINEERING; GLYCOSYLATION; HUMANS; MUCIN-1; N-ACETYLGALACTOSAMINYLTRANSFERASES; PLANTS, GENETICALLY MODIFIED; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; RNA, PLANT; TOBACCO; TRANSFORMATION, GENETIC;

EID: 77955812183     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-62     Document Type: Article

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