Inducible gene and shRNA expression in resident hematopoietic stem cells in vivo

Stem Cells

Volumn 28, Issue 8, 2010, Pages 1390-1398

  Laurenti, Elisa ^a,b   Barde, Isabelle ^a   Verp, Sonia ^a   Offner, Sandra ^a   Wilson, Anne ^b   Quenneville, Simon ^a   Wiznerowicz, Maciej ^a   MacDonald, H Robson ^b   Trono, Didier ^a   Trumpp, Andreas ^a,c,d  

^a EPFL   (Switzerland)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^d Heidelberg Institute for Stem Cell Technology and Experimental Medicine HI STEM   (Germany)

Author keywords

Doxycycline; Hematopoiesis; Hematopoietic stem cells; In vivo inducible genetic manipulation; Lentiviral vectors; tTR KRAB

Indexed keywords

COMPLEMENTARY DNA; DOXYCYCLINE; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; PROTEIN P53; REPRESSOR PROTEIN; SHORT HAIRPIN RNA;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENGRAFTMENT; FEMALE; GENE; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENE SILENCING; HEMATOPOIETIC CELL; HEMATOPOIETIC STEM CELL; IN VIVO STUDY; IRRADIATION; MOUSE; NONHUMAN; PROGENY; PROTEIN EXPRESSION; TRANSGENIC MOUSE; TRANSGENICS;

ANIMALS; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CELLS, CULTURED; DOXYCYCLINE; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC VECTORS; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; LENTIVIRUS; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; TUMOR SUPPRESSOR PROTEIN P53;

MUS; MUS MUSCULUS;

EID: 77955797425     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.460     Document Type: Article

References (35)

1. Laurenti E, Varnum-Finney B, Wilson A et al. Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity. Cell Stem cell 2008;3:611-624.
2. Passegue E, Wagers AJ, Giuriato S et al. Global analysis of proliferation and cell cycle gene expression in the regulation of hematopoietic stem and progenitor cell fates. J Exp Med 2005;202:1599-1611. (Pubitemid 41746605)
3. Venezia TA, Merchant AA, Ramos CA et al. Molecular signatures of proliferation and quiescence in hematopoietic stem cells. Plos Biol 2004;2:e301.
4. Wilson A, Laurenti E, Oser G et al. Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair. Cell 2008;135:1118-1129.
5. Essers MA, Offner S, Blanco-Bose WE et al. IFNalpha activates dormant haematopoietic stem cells in vivo. Nature 2009;458:904-908.
6. Sato T, Onai N, Yoshihara H et al. Interferon regulatory factor-2 protects quiescent hematopoietic stem cells from type I interferon-dependent exhaustion. Nat Med 2009;15:696-700.
7. Fukuchi Y, Ito M, Shibata F et al. Activation of CCAAT/enhancer-binding protein alpha or PU. 1 in hematopoietic stem cells leads to their reduced self-renewal and proliferation. Stem Cells 2008;26:3172-3181.
8. Pfeifer A, Brandon EP, Kootstra N et al. Delivery of the Cre recombinase by a self-deleting lentiviral vector: Efficient gene targeting in vivo. Proc Natl Acad Sci USA 2001;98:11450-11455.
9. Gothert JR, Gustin SE, van Eekelen JA et al. Genetically tagging endothelial cells in vivo: Bone marrow-derived cells do not contribute to tumor endothelium. Blood 2004;104:1769-1777.
10. Hartner JC, Walkley CR, Lu J et al. ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling. Nat Immunol 2009;10:109-115.
11. Bockamp E, Antunes C, Maringer M et al. Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells. Blood 2006;108:1533-1541.
12. Koschmieder S, Gottgens B, Zhang P et al. Inducible chronic phase of myeloid leukemia with expansion of hematopoietic stem cells in a transgenic model of BCR-ABL leukemogenesis. Blood 2005;105:324-334.
13. Higashi AY, Ikawa T, Muramatsu M et al. Direct hematological toxicity and illegitimate chromosomal recombination caused by the systemic activation of CreERT2. J Immunol 2009;182:5633-5640.
14. Cudre-Mauroux C, Occhiodoro T, Konig S et al. Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability. Hum Gene Ther 2003;14:1525-1533. (Pubitemid 37357740)
15. Wiznerowicz M, Jakobsson J, Szulc J et al. The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesis. The Journal Of Biological Chemistry 2007;282:34535-34541.
16. VandenDriessche T, Thorrez L, Naldini L et al. Lentiviral vectors containing the human immunodeficiency virus type-1 central polypurine tract can efficiently transduce nondividing hepatocytes and antigen-presenting cells in vivo. Blood 2002;100:813-822.
17. Eve DJ, Dennis JS, Citron BA. Transcription factor p53 in degenerating spinal cords. Brain Res 2007;1150:174-181.
18. Wiznerowicz M, Szulc J, Trono D. Tuning silence: Conditional systems for RNA interference. Nat Methods 2006;3:682-688. (Pubitemid 44275734)
19. Wu RH, Cheng TL, Lo SR et al. A tightly regulated and reversibly inducible siRNA expression system for conditional RNAi-mediated gene silencing in mammalian cells. J Gene Med 2007;9:620-634.
20. Szulc J, Wiznerowicz M, Sauvain MO et al. A versatile tool for conditional gene expression and knockdown. Nat Methods 2006;3:109-116.
21. Dickins RA, Hemann MT, Zilfou JT et al. Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat Genet 2005;37:1289-1295.
22. Dickins RA, McJunkin K, Hernando E et al. Tissue-specific and reversible RNA interference in transgenic mice. Nat Genet 2007;39:914-921.
23. Barde I, Zanta-Boussif MA, Paisant S et al. Efficient control of gene expression in the hematopoietic system using a single Tet-on inducible lentiviral vector. Mol Ther 2006;13:382-390.
24. Wiznerowicz M, Trono D. Conditional suppression of cellular genes: Lentivirus vector-mediated drug-inducible RNA interference. J Virol 2003;77:8957-8961. (Pubitemid 36936105)
25. Deuschle U, Meyer WK, Thiesen HJ. Tetracycline-reversible silencing of eukaryotic promoters. Mol Cell Biol 1995;15:1907-1914.
26. Centlivre M, Zhou X, Pouw SM et al. Autoregulatory lentiviral vectors allow multiple cycles of doxycycline-inducible gene expression in human hematopoietic cells in vivo. Gene Ther 17:14-25.
27. Shin KJ, Wall EA, Zavzavadjian JR et al. A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Proc Natl Acad Sci USA 2006;103:13759-13764.
28. Brown BD, Cantore A, Annoni A et al. A microRNA-regulated lentiviral vector mediates stable correction of hemophilia B mice. Blood 2007;110:4144-4152.
29. Brown BD, Gentner B, Cantore A et al. Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state. Nat Biotechnol 2007;25:1457-1467.
30. Borman AM, Le Mercier P, Girard M et al. Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins. Nucleic Acids Res 1997;25:925-932. (Pubitemid 27298259)
31. Mizuguchi H, Xu Z, Ishii-Watabe A et al. IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol Ther 2000;1:376-382.
32. Milch RA, Rall DP, Tobie JE. Bone localization of the tetracyclines. J Natl Cancer Inst 1957;19:87-93.
33. Buyske DA, Eisner HJ, Kelly RG. Concentration and persistence of tetracycline and chlortetracycline in bone. J Pharmacol Exp Ther 1960;130:150-156.
34. Soucek L, Whitfield J, Martins CP et al. Modelling Myc inhibition as a cancer therapy. Nature 2008;455:679-683.
35. Barde I, Laurenti E, Verp S et al. Regulation of episomal gene expression by KRAB/KAP1-mediated histone modifications. J Virol 2009;83:5574-5580.