Cloning of the RNase H genes from a metagenomic DNA library: Identification of a new type 1 RNase H without a typical active-site motif

Journal of Applied Microbiology

Volumn 109, Issue 3, 2010, Pages 974-983

  Kanaya, E ^a   Sakabe, T ^a   Nguyen, N T ^a   Koikeda, S ^b   Koga, Y ^a   Takano, K ^a,c   Kanaya, S ^a  

^a OSAKA UNIVERSITY   (Japan)

^b Amano Enzyme Inc   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

compost; metagenomic DNA library; phylogenetic analysis; RNase H; screening

Indexed keywords

CLONING; COMPOSTING; DNA; DNA SEQUENCES; ENCODING (SYMBOLS); ESCHERICHIA COLI;

ACTIVE SITE; COMPOST; DNA LIBRARY; METAGENOMIC DNA; METAGENOMIC DNA LIBRARY; METAGENOMICS; PHYLOGENETIC ANALYSIS; RNASE H; SEQUENCE SIMILARITY; TEST CASE;

GENES;

DNA FRAGMENT; RIBONUCLEASE H;

AMINO ACID; BACTERIUM; CATALYSIS; COLIFORM BACTERIUM; COMPOST; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ENGINEERING; GENOMICS; PHENOTYPE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DATA BASE; DNA LIBRARY; DNA SEQUENCE; ESCHERICHIA COLI; GENETIC CODE; GENETIC SCREENING; METAGENOMICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; TEMPERATURE SENSITIVE MUTANT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77955787602     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04724.x     Document Type: Article

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