A facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts

Journal of Neuroscience Methods

Volumn 191, Issue 2, 2010, Pages 222-226

  Petty, Howard R ^a   Elner, Victor M ^a   Kawaji, Takahiro ^a   Clark, Andrea ^a   Thompson, Debra ^a   Yang, Dong Li ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Fluorescence; Human; Nanoparticles; Retina

Indexed keywords

ENOLASE; LIPOFUSCIN; MELANIN; NANOPARTICLE; QUANTUM DOT;

ANTIGEN DETECTION; ARTICLE; AUTOFLUORESCENCE; BRIGHT FIELD MICROSCOPY; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; PIGMENT EPITHELIUM; PRIORITY JOURNAL; RETINA;

AGED; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; HUMANS; LIGHT; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; NANOPARTICLES; NEURONS; PHOTIC STIMULATION; PHOTOCHEMISTRY; QUANTUM DOTS; RETINA; STAINING AND LABELING;

EID: 77955770553     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2010.07.001     Document Type: Article

References (12)

1. Boellaard J.W., Schlote W., Hofer W. Species-specific ultrastructure of neuronal lipofuscin in hippocampus and neocortex of subhuman mammals and humans. Ultrastruct Pathol 2004, 28:341-351.
2. Benson D.M., Bryan J., Plant A.L., Botto A.M., Smith L.C. Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells. J Cell Biol 1985, 100:1309-1323.
3. Delori F.C., Goger D.G., Dorey C.K. Age-related accumulation and spatial distribution of lipofuscin in RPE of normal subjects. Invest Ophthalmol Vis Sci 2001, 42:1855-1866.
4. Elner V.M., Elner S.G., Bian Z.M., Kindezelskii A.L., Yoshida A., Petty H.R. RPE CD14 immunohistochemical, genetic, and functional expression. Exp Eye Res 2003, 76:321-331.
5. Gao X., Yang L., Petros J.A., Marshall F.F., Simons J.W., Nie S. In vivo molecular and cellular imaging with quantum dots. Curr Opin Biotechnol 2005, 16:63-72.
6. Igarashi Y., Ishii T. Lipofuscin pigments in the spiral ganglion of the rat. Eur Arch Otorhinolaryngol 1990, 247:189-193.
7. Michalet X., Pinaud F.F., Bentolila L.A., Tsay J.M., Doose S., Li J.J., et al. Quantum dots for live cells, in vivo imaging, and diagnostic. Science 2005, 307:538-544.
8. Mullins R.F., Kuehn M.H., Faidley E.A., Syed N.A., Stone E.M. Differential macular and peripheral expression of bestrophin in human eyes and its implication for best disease. Invest Ophthalmol Vis Sci 2007, 48:3372-3380.
9. Petty H.R. Fluorescence microscopy, emerging methods and strategies to extract weak signals with applications in immunology. Microsc Res Techn 2007, 70:687-709.
10. Puchtler H., Meloan S.N., Paschal L.D. Infrared fluorescence microscopy of stained tissues: principles and technic. Histochemistry 1980, 68:211-230.
11. Sall J.W., Klisovic D.D., O'Dorisio M.S., Katz S.E. Somatostatin inhibits IGF-1 mediated induction of VEGF in human retinal pigment epithelial cells. Exp Eye Res 2004, 79:465-476.
12. Schnell S.A., Staines W.A., Wessendorf M.W. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J Histochem Cytochem 1999, 47:719-730.