A flow cytometry-based assay to assess minute frequencies of CD8+ T cells by their cytolytic function

Journal of Immunological Methods

Volumn 360, Issue 1-2, 2010, Pages 56-65

  Stanke, Jonas ^a   Hoffmann, Corinna ^a   Erben, Ulrike ^a   von Keyserling, Helmut ^a   Stevanovic, Stefan ^b   Cichon, Guenter ^a   Schneider, Achim ^a   Kaufmann, Andreas M ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

CFSE; Chromium release; Cytotoxicity assay; Far Red; Immuno monitoring; In vitro incubation; Kill assay

Indexed keywords

CHROMIUM; EPITOPE; HLA A2 ANTIGEN; FLUORESCENT DYE;

ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; CD8+ T LYMPHOCYTE; CELL LABELING; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXIC T LYMPHOCYTE; ERYTHROLEUKEMIA CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL; LYMPHOCYTE FUNCTION; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SOMATIC CELL; CELL COUNT; COMPARATIVE STUDY; CYTOTOXICITY; CYTOTOXICITY TEST; GENETICS; IMMUNOLOGICAL MONITORING; IMMUNOLOGY; K562 CELL LINE; LYMPHOCYTE ACTIVATION; METABOLISM; PATHOLOGY; PROCEDURES; REPRODUCIBILITY; SAMPLE SIZE; SENSITIVITY AND SPECIFICITY; TRANSGENE;

CD8-POSITIVE T-LYMPHOCYTES; CELL COUNT; CYTOTOXICITY TESTS, IMMUNOLOGIC; CYTOTOXICITY, IMMUNOLOGIC; FLOW CYTOMETRY; FLUORESCENT DYES; HLA-A2 ANTIGEN; HUMANS; K562 CELLS; LYMPHOCYTE ACTIVATION; MONITORING, IMMUNOLOGIC; REPRODUCIBILITY OF RESULTS; SAMPLE SIZE; SENSITIVITY AND SPECIFICITY; TRANSGENES;

EID: 77955654862     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.06.005     Document Type: Article

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