Engineering of Escherichia coli L-serine O-acetyltransferase on the basis of crystal structure: Desensitization to feedback inhibition by L-cysteine

Protein Engineering, Design and Selection

Volumn 19, Issue 4, 2006, Pages 163-167

  Kai, Y ^a   Kashiwagi, T ^a   Ishikawa, K ^a   Ziyatdinov, M K ^b   Redkina, E I ^b   Kiriukhin, M Y ^b   Gusyatiner, M M ^b   Kobayashi, S ^c   Takagi, H ^c   Suzuki, E ^a  

^a AJINOMOTO CO INC   (Japan)

^b Ajinomoto Genetika Research Institute   (Russian Federation)

^c FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Desensitization; Escherichia coli; Feedback inhibition; L cysteine; L serine O acetyltransferase

Indexed keywords

CATALYSIS; CRYSTAL STRUCTURE; ENZYMES; FERMENTATION; REACTION KINETICS; SUBSTRATES; SYNTHESIS (CHEMICAL);

DESENSITIZATION; FEEDBACK INHIBITION; L-CYSTEINE; L-SERINE O-ACETYLTRANSFERASE;

ESCHERICHIA COLI;

ASPARTIC ACID; CYSTEINE; SERINE;

AMINO ACID SUBSTITUTION; AMINO ACID SYNTHESIS; ARTICLE; BINDING AFFINITY; BIOENGINEERING; CONTROLLED STUDY; CRYSTAL STRUCTURE; ESCHERICHIA COLI; FERMENTATION TECHNIQUE; GENE MUTATION; MUTAGENESIS; MUTANT; NEGATIVE FEEDBACK; NONHUMAN; PRIORITY JOURNAL; RANDOMIZATION;

AMINO ACID SUBSTITUTION; CYSTEINE; ESCHERICHIA COLI; FEEDBACK, BIOCHEMICAL; INHIBITORY CONCENTRATION 50; SERINE O-ACETYLTRANSFERASE;

ESCHERICHIA COLI;

EID: 33644884736     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzj015     Document Type: Article

References (19)

1. Denk,D. and Böck,A. (1987) J. Gen. Microbiol., 133, 515-525.
2. Hindson,V.J. (2003) Biochem. J., 375, 745-752.
3. Hindson,V.J. and Shaw,W.V. (2003) Biochemistry, 42, 3113-3110.
4. Ishikawa,K., Mihara,Y., Gondoh,K., Suzuki,E. and Asano,Y. (2000) EMBO J., 19, 2412-2423.
5. Ishikawa,K., Mihara,Y., Shimba,N., Ohtsu,N., Kawasaki,H., Suzuki,E. and Asano,Y. (2002) Protein Eng., 15, 539-543.
6. Kredich,N.M. and Tomkins,G.M. (1966) J. Biol. Chem., 241, 4955-4965.
7. Kredich,N.M., Becker,M.A. and Tomkins,G.M. (1969) J. Biol. Chem., 244, 2428-2433.
8. Kuntz,I.D., Meng,E.C. and Shoichet,B.K.(1994) Acc. Chem. Res., 27, 117-123.
9. Kushner,S.R., Nagaishi,H. and Clark,A.J. (1972) Proc. Natl Acad. Sci. USA, 69, 1366-1370.
10. Moody,P.C.E. and Wilkinson,J.A. (1990) In Rickwood,E. (ed.), Protein Engineering in Focus. IRL Press, Oxford, UK.
11. Nakamori,S., Kobayashi,S., Kobayashi,C. and Takagi,H. (1998) Appl. Environ. Microbiol., 64, 1607-1611.
12. Nakamura,K., Hayama,A., Masada,M., Fukushima,K. and Tamura,G. (1987) Plant Cell Physiol., 28, 885-891.
13. Noji,M., Inoue,K., Kimura,N., Gouda,A. and Saito,K. (1998) J. Biol. Chem., 273, 32739-32745.
14. Pye,V.E., Tingey,A.P., Robson,R.L. and Moody,P.C.E. (2004) J. Biol. Chem., 279, 40729-40736.
15. Sambrook,J. and Russell,D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
16. Smith,J.K. and Thompson,J.F. (1969). Biochem. Biophys. Res. Commun., 35, 939-945.
17. Soda,K. (1987) An overview in Methods in Enzymology. Academic Press, New York.
18. Takagi,H., Awano,N., Kobayashi,S., Noji,M., Saito,K. and Nakamori,S. (1999a) FEMS Microbiol. Lett., 179, 453-459.
19. Takagi,H., Kobayashi,C., Kobayashi,S. and Nakamori,S. (1999b) FEBS Lett., 452, 323-327.