PCR random mutagenesis into Escherichia coli serine acetyltransferase: Isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition

FEBS Letters

Volumn 452, Issue 3, 1999, Pages 323-327

  Takagi, Hiroshi ^a   Kobayashi, Chitose ^a   Kobayashi, Shin Ichiro ^a   Nakamori, Shigeru ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Feedback inhibition; L Cysteine overproduction; Random mutagenesis; Serine acetyltransferase

Indexed keywords

CYSTEINE;

ARTICLE; CONTROLLED STUDY; DRUG SYNTHESIS; ESCHERICHIA COLI; MUTAGENESIS; NEGATIVE FEEDBACK; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ACETYLTRANSFERASES; AMINO ACID SUBSTITUTION; CYSTEINE; CYSTINE; DNA PRIMERS; ESCHERICHIA COLI; FEEDBACK; KINETICS; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SERINE O-ACETYLTRANSFERASE;

ESCHERICHIA COLI;

EID: 0033004153     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(99)00679-1     Document Type: Article

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