Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning

Chemico-Biological Interactions

Volumn 187, Issue 1-3, 2010, Pages 380-383

  Jun, Daniel ^a,b   Musilová, Lucie ^c   Link, Marek ^d   Loiodice, Mélanie ^e   Nachon, Florian ^e   Rochu, Daniel ^e   Renault, Frédérique ^e   Masson, Patrick ^e  

^a UNIVERSITY OF DEFENCE   (Czech Republic)

^b CZECH UNIVERSITY OF LIFE SCIENCES PRAGUE   (Czech Republic)

^c Charles University in Prague Faculty of Pharmacy   (Czech Republic)

^d UNIVERSITY OF DEFENCE   (Czech Republic)

^e INSTITUT DE RECHERCHE BIOMÉDICALE DES ARMÉES   (France)

Author keywords

Acetylcholinesterase; Bioscavenger; Butyrylcholinesterase; Organophosphate; Phosphotriesterase; Scavenger

Indexed keywords

ALDEHYDE; BACTERIAL ENZYME; ORGANOPHOSPHATE; PHOSPHOTRIESTERASE MACROGOL; SCAVENGER; UNCLASSIFIED DRUG;

ARTICLE; BIOLOGICAL ACTIVITY; CATALYSIS; CHEMICAL MODIFICATION; CONTROLLED STUDY; DISTRIBUTION VOLUME; DRUG STABILITY; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME MODIFICATION; ESCHERICHIA COLI; IMMUNOGENICITY; INTOXICATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; REACTION OPTIMIZATION; SCAVENGING SYSTEM; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77955516214     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2010.03.017     Document Type: Article

References (20)

1. Sultatos L.G. Mammalian toxicology of organophosphorus pesticides. J. Toxicol. Environ. Health 1994, 43:271-289.
2. Costa L.G. Current issues in organophosphate toxicology. Clin. Chim. Acta 2006, 366:1-13.
3. Bajgar J., Fusek J., Kuca K., Bartosova L., Jun D. Treatment of organophosphate intoxication using cholinesterase reactivators: facts and fiction. Mini Rev. Med. Chem. 2007, 7:461-466.
4. Worek F., Aurbek N., Wetherell J., Pearce P., Mann T., Thiermann H. Inhibition, reactivation and aging kinetics of highly toxic organophosphorus compounds: pig versus minipig acetylcholinesterase. Toxicology 2008, 244:35-41.
5. Bajgar J, Fusek J., Kassa J., Kuca K., Jun D. Chemical aspects of pharmacological prophylaxis against nerve agent poisoning. Curr. Med. Chem. 2009, 16:2977-2986.
6. Kuca K., Cabal J., Jung Y.S., Musilek K., Soukup O., Jun D., Pohanka M., Musilova L., Karasova J., Novotny L., Hrabinova M. Reactivation of human brain homogenate cholinesterases inhibited by Tabun using newly developed oximes K117 and K127. Basic Clin. Pharmacol. Toxicol. 2009, 105:207-210.
7. Musilova L., Kuca K., Jung Y.S., Jun D. In vitro oxime-assisted reactivation of paraoxon-inhibited human acetylcholinesterase and butyrylcholinesterase. Clin. Toxicol. (Phila). 2009, 47:545-550.
8. Masson P., Rochu D. Catalytic Bioscavengers: The Next Generation of Bioscavenger-Based Medical Countermeasures. Handbook of Toxicology of Chemical Warfare Agents 2009, 1053-1065. Academic Press, San Diego, CA. R. Gupta (Ed.).
9. Doctor B.P., Saxena A. Bioscavengers for the protection of humans against organophosphate toxicity. Chem. Biol. Interact. 2005, 157-158:167-171.
10. Omburo G.A., Kuo J.M., Mullins L.S., Raushel F.M. Characterization of the zinc binding site of bacterial phosphotriesterase. J. Biol. Chem. 1992, 267:13278-13283.
11. Petrikovics I., Cheng T.C., Papahadjopoulos D., Hong K., Yin R., DeFrank J.J., Jaing J., Song Z.H., McGuinn W.D., Sylvester D., Pei L., Madec J., Tamulinas C., Jaszberenyi J.C., Barcza T., Way J.L. Long circulating liposomes encapsulating organophosphorus acid anhydrolase in diisopropylfluorophosphate antagonism. Toxicol. Sci. 2000, 57:16-21.
12. Tuovinen K., Kaliste-Korhonen E., Raushel F.M., Hanninen O. Eptastigmine-phosphotriesterase combination in DFP intoxication. Toxicol. Appl. Pharmacol. 1996, 140:364-369.
13. Cohen O., Kronman C., Chitlaru T., Ordentlich A., Velan B., Shafferman A. Effect of chemical modification of recombinant human acetylcholinesterase by polyethylene glycol on its circulatory longevity. Biochem. J. 2001, 357:795-802.
14. Kronman C., Cohen O., Raveh L., Mazor O., Ordentlich A., Shafferman A. Polyethylene-glycol conjugated recombinant human acetylcholinesterase serves as an efficacious bioscavenger against soman intoxication. Toxicology 2007, 233:40-46.
15. Hinds K.D., Kim S.W. Effects of PEG conjugation on insulin properties. Adv. Drug Deliv. Rev. 2002, 54:505-530.
16. Rochu D., Beaufet N., Renault F., Viguie N., Masson P. The wild type bacterial Co(2+)/Co(2+)-phosphotriesterase shows a middle-range thermostability. Biochim. Biophys. Acta 2002, 1594:207-218.
17. Rochu D., Viguie N., Renault F., Crouzier D., Froment M.T., Masson P. Contribution of the active-site metal cation to the catalytic activity and to the conformational stability of phosphotriesterase: temperature- and pH-dependence. Biochem. J. 2004, 380:627-633.
18. Sasatsu M., Onishi H., Machida Y. Preparation of a PLA-PEG block copolymer using a PLA derivative with a formyl terminal group and its application to nanoparticulate formulation. Int. J. Pharm. 2005, 294:233-299.
19. Bailon P., Berthold W. Polyethylene glycol-conjugated pharmaceutical proteins. Pharm. Sci. Technol. Toxicol. 1998, 1:352-356.
20. Harris J.M., Chess R.B. Effect of pegylation on pharmaceuticals. Nat. Rev. Drug Discov. 2003, 2:214-221.