Construction of a multiple targeting RNAi plasmid that inhibits target gene expression and FMDV replication in BHK-21 cells and suckling mice

Veterinary Research Communications

Volumn 34, Issue 4, 2010, Pages 335-346

  Cong, Wei ^a   Cui, Shaoqiang ^a   Chen, Jiulian ^b   Zuo, Xiaoping ^b   Lu, Yonggan ^b   Yan, Weiyao ^a   Zheng, Zhaoxin ^a  

^a FUDAN UNIVERSITY   (China)

^b Bio pharmacy   (China)

Author keywords

FMDV; Multiple targeting plasmid; RNA interference (RNAi); SiRNA; VP1 gene

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; PLASMID VECTOR; PROTEIN VP1; SMALL INTERFERING RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIVIRAL ACTIVITY; ARTICLE; CELL STRAIN BHK; CONTROLLED STUDY; DRUG EFFICACY; FOOT AND MOUTH DISEASE; FOOT AND MOUTH DISEASE VIRUS; GENE EXPRESSION REGULATION; GENE SILENCING; GENE TARGETING; INFECTION SENSITIVITY; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; NUCLEOTIDE SEQUENCE; REPORTER GENE; RNA INTERFERENCE; VIRUS GENE; VIRUS REPLICATION; ANIMAL; CELL LINE; DRUG EFFECT; GENE EXPRESSION; HAMSTER; KIDNEY; PHYSIOLOGY; PLASMID; SUCKLING;

ANIMALS; ANIMALS, SUCKLING; CELL LINE; CRICETINAE; FOOT-AND-MOUTH DISEASE; FOOT-AND-MOUTH DISEASE VIRUS; GENE EXPRESSION; KIDNEY; MICE; PLASMIDS; RNA INTERFERENCE; VIRUS REPLICATION;

ANIMALIA; FOOT-AND-MOUTH DISEASE VIRUS; MUS;

EID: 77955504638     PISSN: 01657380     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11259-010-9360-y     Document Type: Article

References (35)

1. Bachrach HL (1968) Foot-and-mouth disease virus. Annu. Rev. Microbiol. 22: 201-224.
2. Bernstein E., Caudy A.A., Hammond S.M., Hannon G.J. (2001a) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409: 363-366.
3. Bernstein E., Denli A.M., Hannon G.J. (2001b) The rest is silence. RNA 7: 1509-1521.
4. Brummelkamp T.R., Bernards R., Agami R. (2002) Asystem for stable expression of short interfering RNAs in mammalian cells. Science 296: 550-553.
5. Chen W, Yan W, Du Q, Fei L, Liu M, Ni Z, Sheng Z, Zheng Z (2004) RNA interference targeting VP1 inhibits foot-and-mouth disease virus replication in BHK-21 cells and suckling mice. J. Virol. 78: 6900-6907.
6. Chang LJ, Liu X, He J (2005) Lentiviral siRNAs targeting multiple highly conserved RNA sequences of human immunodeficiency virus type 1. Gene Ther. 12: 1133-1144.
7. Dumbell KR, Jarrett JO, Mautner V, Minson AC, Pereira HG, Pereira MS, Porterfield JS, Wilkinson PJ (1989) Picornaviridae. In: Porterfield JS (ed) Andrewes' viruses of vertebrates, 5th edn. Cambridge University Press, Cambridge, United Kingdom, pp 120-145.
8. Ding H, Schwarz DS, Keene A, Affar EB, Fenton L, Xia X, Shi Y, Zamore PD, Xu Z (2003) Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosis. Aging Cell 2: 209-217.
9. Das AT, Brummelkamp TR, Westerhout EM, Vink M, Madiredjo M, Bernards R, Berkhout B (2004) Human Immunodeficiency Virus type 1 escapes from RNA interference-mediated inhibition. J. Virol. 78: 2601-2605. (Pubitemid 38228957)
10. Dave RS, Pomerantz RJ (2004) Antiviral effects of Human Immunodeficiency Virus type 1-specific small interfering RNAs against targets conserved in select neurotropic viral strains. J. Virol. 78: 13687-13696.
11. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T (2001a) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 379 494-498.
12. Elbashir SM, Lendeckel W, Tuschl T (2001b) RNA interference is mediated by 21-and 22-nucleotide RNAs. Genes Dev. 15: 188-200. (Pubitemid 32107664)
13. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811. (Pubitemid 28099681)
14. Gitlin L, Andino R (2003) Nucleic acid-based immune system: the antiviral potential of mammalian RNA silencing. J. Virol. 77: 7159-7165.
15. Gitlin L, Stone JK, Andino R (2005) Poliovirus escape from RNA interference: short interfering RNA-target recognition and implications for therapeutic approaches. J. Virol. 79: 1027-1035.
16. Grubman M, Santos T (2005) Rapid control of foot-and-mouth disease outbreaks:is RNAi a possible solution? TRENDS in Immunology 26: 65-68.
17. Geisbert TW, Hensley LE, Kagan E, Yu EZ, Geisbert JB, Daddario-DiCaprio K, Fritz EA, Jahrling PB, McClintock K, Phelps JR, Lee ACH, Judge A, Jeffs LB, MacLachlan I (2006) Postexposure protection of guinea pigs against a lethal Ebola virus challenge is conferred by RNA interference. J. Infect. Dis. 193: 1650-1657.
18. Jackson AL, Bartz SR, Schelter J, Kobayashi SV, Burchard J, Mao M, Li B, Cavet G, Linsley PS (2003) Expression profiling reveals off-target gene regulation by RNAi. Nat. Biotechnol. 21: 635-637.
19. Kennerdell JR, Carthew RW (2000) Heritable gene silencing in Drosophila using double-stranded RNA. Nat. Biotechnol. 18: 896-898.
20. Knight SW, Bass BL (2001) A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans. Science 293: 2269-2271.
21. Kahana R, Kuznetzova L, Rogel A, Shemesh M, Hai D, Yadin H, Stram Y (2004) Inhibition of foot-andmouth disease virus replication by small interfering RNA. J. Gen. Virol. 85: 3213-3217.
22. Liu M, Chen W, Ni Z, Yan W, Fei L, Jiao Y, Zhang J, Du Q, Wei X, Chen J, Liu Y, Zheng Z (2005) Crossinhibition to heterologous foot and- mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome. Virology 336: 51-59.
23. Macpherson I, Stoker M (1962) Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence. Virology 16: 147-151.
24. Mohapatra JK, Sanyal A, Hemadri D, Tosh C, Kumar RM, Bandyopadhyay SK (2005) Evaluation of in vitro inhibitory potential of small interfering RNAs directed against various regions of foot-and-mouth disease virus genome. Biochem. Biophys. Res. Commun. 329: 1133-1138.
25. Nishitsuji H, Kohara M, Kannagi M, Masuda T (2006) Effective suppression of human immunodeficiency virus type 1 through a combination of short- or long-hairpin RNAs targeting essential sequences for retroviral integration. J. Virol. 80: 7658-7666.
26. Paul CP, Good PD, Winer I, Engelke DR (2002) Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20: 505-508.
27. Phipps KM, Martinez A, Lu J, Heinz BA, Zhao G (2004) Small interfering RNA molecules as potential antihuman rhinovirus agents: in vitro potency, specificity, and mechanism. Antiviral Res. 61: 49-55.
28. Reed LJ, Muench HA (1938) A simple method of estimating fifty percent end points. Am. J. Hyg. 27: 493-497.
29. Sharp PA (2001) RNA interference-2001. Genes Dev. 15: 485-490.
30. Sui G, Soohoo C, Affar B, Gay F, Shi Y, Forrester WC, Shi Y (2002) A DNAvector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 99: 5515-5520.
31. Song E, Lee SK, Dykxhoorn DM, Novina C, Zhang D, Crawford K, Cerny J, Sharp PA, Lieberman J, Manjunath N, Shankar P (2003) Sustained small interfering RNA-mediated human immunodeficiency virus type 1 inhibition in primary macrophages. J. Virol. 77: 7174-7181.
32. Tavernarakis N, Wang SL, Dorovkov M, Ryazanov A, Driscoll M (2000) Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes. Nat. Genet. 24: 180-183.
33. Wu KL, Zhang X, Zhang J, Yang Y, Mu YX, Liu M, Lu L, Li Y, Zhu Y, Wu J (2005) Inhibition of hepatitis B virus gene expression by single and dual small interfering RNA treatment. Virus Res. 112: 100-107.
34. Wu KL, Mu Y, Hu J, Lu L, Zhang X, Yang Y, Li Y, Liu F, Song D, Zhu Y, Wu J (2007) Simultaneously inhibition of HIV and HBV replication through a dual small interfering RNA expression system. Antiviral Res. 74: 142-149.
35. Zhang H, Kolb FA, Brondani V, Billy E, Filipowicz W (2002) Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J. 21: 5875-5885.