Novel in vitro co-culture methodology to investigate heterotypic cell-cell interactions

European Cells and Materials

Volumn 19, Issue , 2010, Pages 166-179

  Burguera, Elena F ^a   Bitar, Malak ^b   Bruinink, Arie ^a  

^a SWISS FEDERAL LABORATORIES FOR MATERIALS SCIENCE AND TECHNOLOGY   (Switzerland)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

2D co culture; Biomaterial surfaces; Cell proliferation; Fibroblasts; In vitro; Osteoblasts

Indexed keywords

ABDOMEN; ARTICLE; BONE; CELL COMMUNICATION; CELL PROLIFERATION; COCULTURE; CYTOLOGY; FIBROBLAST; HUMAN; METHODOLOGY; OSTEOBLAST; TECHNIQUE; VALIDATION STUDY;

ABDOMEN; BONE AND BONES; CELL COMMUNICATION; CELL PROLIFERATION; COCULTURE TECHNIQUES; CYTOLOGICAL TECHNIQUES; FIBROBLASTS; HUMANS; METHODS; OSTEOBLASTS; RESEARCH DESIGN;

EID: 77955484159     PISSN: 14732262     EISSN: None     Source Type: Journal    
DOI: 10.22203/eCM.v019a17     Document Type: Article

References (30)

1. Anderson JM (2001) Biological responses to materials. Annu Rev Mater Res 31: 81-110.
2. Brånemark PI, Hansson BO, Adell R, Breine U, Lindström J, Hallén O, Ohman A (1977) Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period. Scand J Plast Reconstr Surg Suppl 16: 1-132.
3. Carpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, Guertin DA, Chang JH, Lindquist RA, Moffat J, Golland P, Sabatini DM (2006) CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol 7: R100.
4. Cooper LF, Masuda T, Yliheikkila PK, Felton DA (1998) Generalizations regarding the process and phenomenon of osseointegration. Part II. In vitro studies. Int J Oral Maxillofac Implants 13: 163-174.
5. Ferrari A, Hannouche D, Oudina K, Bourguignon M, Meunier A, Sedel L, Petite H (2001) In vivo tracking of bone marrow fibroblasts with fluorescent carbocyanine dye. J Biomed Mater Res 56: 361-367.
6. Franssen EHP, Roet KCD, de Bree FM, Verhaagen J (2009) Olfactory Ensheathing Glia and Schwann Cells Exhibit a Distinct Interaction Behavior With Meningeal Cells. J Neurosci Res 87: 1556-1564.
7. Fuchs S, Hofmann A, Kirkpatrick CJ (2007) Microvessel-like structures from outgrowth endothelial cells from human peripheral blood in 2-dimensional and 3-dimensional co-cultures with osteoblastic lineage cells. Tissue Eng 13: 2577-2588.
8. Gerstenfeld LC, Cruceta J, Shea CM, Sampath K, Barnes GL, Einhorn TA (2002) Chondrocytes provide morphogenic signals that selectively induce osteogenic differentiation of mesenchymal stem cells. J Bone Miner Res 17: 221-230.
9. Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ (2007) Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials. J Biomed Mater Res part B 81B: 153-161.
10. Honig MG, Hume RI (1986) Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J Cell Biol 103: 171-187.
11. Honig MG, Hume RI (1989) DiI and DiO: versatile fluorescent dyes for neuronal labelling and pathway tracing. Trends Neurosci 12: 333-341.
12. Jiang J, Nicoll SB, Lu HH (2005) Co-culture of osteoblasts and chondrocytes modulates cellular differentiation in vitro. Biochem Biophys Res Commun 338: 762-770.
13. Kaiser JP and Bruinink A (2004) Investigating cellmaterial interactions by monitoring and analysing cell migration. J Mater Sci Mater Med 15: 429-435.
14. Kaiser JP, Reinmann A, Bruinink A (2006) The effect of topographic characteristics on cell migration velocity. Biomaterials 27: 5230-5241.
15. Kirkpatrick CJ, Fuchs S, Hermanns MI, Peters K, Unger RE (2007) Cell culture models of higher complexity in tissue engineering and regenerative medicine. Biomaterials 28: 5193-5198.
16. Kirkpatrick CJ, Fuchs S, Peters K, Brochhausen C, Hermanns MI, Unger RE (2006) Visions for regenerative medicine: Interface between scientific fact and science fiction. Artif Organs 30: 822-827.
17. Krtolica A, de Solorzano CO, Lockett S, Campisi J (2002) Quantification of epithelial cells in coculture with fibroblasts by fluorescence image analysis. Cytometry 49: 73-82.
18. Lu HH., Wang INE (2008) Multiscale coculture models for orthopedic interface tissue engineering, in: Biomedical Nanostructures. (Gonsalves KE, Halberstadt CR, Laurencin CT, Nair LS, eds). Wiley, New York. pp 357-373.
19. Nevo Z, Silver J, Chorev Y, Riklis I, Robinson D, Yosipovitch Z (1993) Adhesion characteristics of chondrocytes cultured separately and in cocultures with synovial fibroblasts. Cell Biol Int 17: 255-273.
20. Oberringer M, Meins C, Bubel M, Pohlemann T (2007) A new in vitro wound model based on the co-culture of human dermal microvascular endothelial cells and human dermal fibroblasts. Biol Cell 99: 197-207.
21. Oberringer M, Meins C, Bubel M, Pohlemann T (2008) In vitro wounding: effects of hypoxia and transforming growth factor beta(1) on proliferation, migration and myofibroblastic differentiation in an endothelial cellfibroblast co-culture model. J Mol Histol 39: 37-47.
22. Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG (2007) Animal models for implant biomaterial research in bone: A review. Eur Cells Mater 13: 1-10.
23. Pizzoferrato A, Ciapetti G, Stea S, Cenni E, Arciola CR, Granchi D, Savarino L (1994) Cell culture methods for testing biocompatibility. Clin Mater 15: 173-190.
24. Puleo DA, Nanci A (1999) Understanding and controlling the bone-implant interface. Biomaterials 20: 2311-2321.
25. Sanchez C, Deberg MA, Piccardi N, Msika P, Reginster JYL, Henrotin YE (2005) Subchondral bone osteoblasts induce phenotypic changes in human osteoarthritic chondrocytes. Osteoarthritis Cartilage 13: 988-997.
26. Spalazzi JP, Dionisio KL, Jiang J, Lu HH (2003) Osteoblast and chondrocyte interactions during coculture on scaffolds. IEEE Eng Meg Biol Mag 22: 27-34.
27. Spalazzi JP, Doty SB, Moffat KL, Levine WN, Lu HH (2006) Development of controlled matrix heterogeneity on a triphasic scaffold for orthopedic interface tissue engineering. Tissue Eng 12: 3497-3508.
28. Unger RE, Sartoris A, Peters K, Motta A, Migliaresi C, Kunkel M, Bulnheim U, Rychly J, Kirkpatrick CJ (2007) Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. Biomaterials 28: 3965-3976.
29. Wang INE, Shan J, Choi R, Oh S, Kepler CK, Chen FH, Lu HH (2007) Role of osteoblast-fibroblast interactions in the formation of the ligament-to-bone interface. J Orthop Res 25: 1609-1620.
30. Winn SR, Mitchell J, Uludag H (2006) In vitro testing of biomaterials, in: An Introduction to Biomaterials (Guelcher SA, Hollinger, JO, eds). CRC Press, Boca Raton, FL, pp 63-66.