In vitro wounding: Effects of hypoxia and transforming growth factor β1 on proliferation, migration and myofibroblastic differentiation in an endothelial cell-fibroblast co-culture model

Journal of Molecular Histology

Volumn 39, Issue 1, 2008, Pages 37-47

  Oberringer, Martin ^a   Meins, Claudia ^a   Bubel, Monika ^a   Pohlemann, Tim ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Co culture scratch wound migration assay (CCSWMA); Endothelin 1; Human dermal microvascular endothelial cells (HDMEC); Hypoxia; Myofibroblasts (MF); Normal human dermal fibroblasts (NHDF); Transforming growth factor 1; Wound healing

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; ENDOTHELIN 1; FIBRONECTIN; TRANSFORMING GROWTH FACTOR BETA1; VON WILLEBRAND FACTOR;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL INTERACTION; CELL MIGRATION; CELL POPULATION; CELL PROLIFERATION; CONTROLLED STUDY; ENDOTHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; HYPOXIA; IMMUNOCYTOCHEMISTRY; MICROVASCULATURE; PRIORITY JOURNAL; PROTEIN FUNCTION; WOUND; WOUND HEALING;

CELL COUNT; CELL DIFFERENTIATION; CELL HYPOXIA; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; COCULTURE TECHNIQUES; ENDOTHELIAL CELLS; ENDOTHELIN-1; FIBROBLASTS; HUMANS; IMMUNOHISTOCHEMISTRY; MODELS, BIOLOGICAL; PROTEIN TRANSPORT; TIME FACTORS; TRANSFORMING GROWTH FACTOR BETA1;

EID: 38349189126     PISSN: 15672379     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10735-007-9124-3     Document Type: Article

References (43)

1. Amadeu TP, Coulomb B, Desmouliere A, Costa AMA (2003) Cutaneous wound healing: myofibroblastic differentiation and in vitro models. Int J Low Extrem Wounds 2(2):60-68
2. Basilico N, Speciale L, Parapini S, Ferrante P, Taramelli D (2002) Endothelin-1 production by a microvascular endothelial cell line treated with Plasmodium falciparum parasitized red blood cells. Clin Sci 103(48):464-466
3. Brunner G, Blakytny R (2004) Extracellular regulation of TGF-ß activity in wound repair: growth factor latency as a sensor mechanism for injury. Thromb Haemost 92:253-261
4. Chen CP, Yang YC, Su TH, Chen CY, Aplin JD (2005) Hypoxia and transforming growth factor-β1 act independently to increase extracellular matrix production by placental fibroblasts. J Clin Endocrinol Metab 90:1083-1090
5. Chin GA, Diegelmann RF, Schultz GS (2005) Cellular and molecular regulation of wound healing. Wound Healing 2005:17-39
6. Cordeiro MF, Bhattacharya SS, Schultz GS, Khaw PT (2000) TGF-β1, -β2, and -β3 in vitro: biphasic effects on tenon's fibroblast contraction, proliferation and migration. Invest Ophthalmol Vis Sci 41:756-763
7. Desmouliere A, Geinoz A, Gabbiani F, Gabbiani G (1993) Transforming growth factor-β1 induces α-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol 122:103-111
8. Desmouliere A, Redard M, Darby I, Gabbiani G (1995) Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar. Am J Pathol 146(1):56-66
9. Diegelmann RF, Evans MC (2004) Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 9:283-289
10. Dube J, Chakir J, Dube C, Grimard Y, Laviolette M, Boulet LP (2000) Synergistic action of endothelin (ET)-1 on the activation of bronchial fibroblast isolated from normal and asthmatic subjects. Int J Exp Path 81:429-437
11. Ellis I, Banyard J, Schor SL (1997) Differential response of fetal and adult fibroblasts to cytokines: cell migration and hyaluronan synthesis. Development 124:1593-1600
12. Eul B, Rose F, Krick S, Savai R, Goyal P, Klepetko W, Grimminger F, Weissmann N, Seeger W, Hänze J (2006) Impact of HIF-1α and HIF-2α on proliferation and migration of human pulmonary artery fibroblasts in hypoxia. FASEB J 20(1):163-165
13. Gabbiani G (2003) The myofibroblast in wound healing and fibrocontractive diseases. J Pathol 200(4):500-503
14. Gallelli L, Pelaia G, D'Agostino B, Cuda G, Vatrella A, Fratto D, Gioffre V, Galderisi U, De Nardo M, Mastruzzo C, Salinaro ET, Maniscalco M, Sofia M, Crimi N, Rossi F, Caputi M, Costanzo FS, Maselli R, Marsico SA, Cancheri C (2005) Endothelin-1 induces proliferation of human lung fibroblasts and IL-11 secretion through an ET(A) receptor-dependent activation of MAP kinases. J Cell Biochem 96(4):858-68
15. Gharaee-Kermani M, Phan SH (2001) Role of cytokines and cytokine therapy in wound healing and fibrotic diseases. Curr Pharm Des 7:1083-1103
16. Hogg N, Browning J, Howard T, Winterford C, Fitzpatrick D, Gobe G (1999) Apoptosis in vascular endothelial cells caused by serum deprivation, oxidative stress and transforming growth factor-beta. Endothelium 7(1):35-49
17. Iruela-Arispe ML, Sage EH (1993) Endothelial cells exhibiting angiogenesis in vitro proliferate in response to TGF-beta 1. J Cell Biochem 52(4):414-430
18. Kernochan LE, Tran BN, Tangkijvanich P, Melton AC, Tam SP, Yee HF (2002) Endothelin-1 stimulates human colonic myofibroblast contraction and migration. Gut 50:65-70
19. Kourembanas S, Marsden PA, McQuillan LP, Faller DV (1991) Hypoxia induces endothelin gene expression and secretion in cultured human endothelium. J Clin Invest 88:1054-1057
20. Li W, Li Y, Guan S, Fan J, Cheng CF, Bright AM, Chinn C, Chen M, Woodley DT (2007) Extracellular heat shock protein-90a: linking hypoxia to skin cell motility and wound healing. The EMBO Journal 2007:1-13
21. Masur SK, Dewal HS, Dinh TT, Erenburg I, Petridou S (1996) Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci USA 93(9):4219-4223
22. Merwin JR, Newman W, Beall LD, Tucker A, Madri J (1991) Vascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro. Am J Pathol 138(1):37-51
23. Minchenko A, Caro J (2000) Regulation of endothelin-1 gene expression in human microvascular endothelial cells by hypoxia and cobalt: Role of hypoxia responsive element. Mol Cell Biochem 208:53-62
24. 1 receptor expression and signal transduction in human dermal fibroblasts: impact on cell migration. J Cell Physiol 190:259-265
25. Moulin V, Castilloux G, Auger FA, Garrel D, O'Connor-McCourt MD, Germain L (1998) Modulated response to cytokines of human wound healing myofibroblasts compared to dermal fibroblasts. Exp Cell Res 238:283-293
26. Oberringer M, Jennewein M, Motsch SE, Pohlemann T, Seekamp A (2005) Different cell cycle responses of wound healing protagonists to transient in vitro hypoxia. Histochem Cell Biol 123(6):595-603
27. Oberringer M, Meins C, Bubel M, Pohlemann T (2007) A new in vitro wound model based on the co-culture of human dermal microvascular endothelial cells and human dermal fibroblasts. Biol Cell 99(4):197-207
28. Petzelbauer E, Springhorn JP, Tucker AM, Madri JA (1996) Role of plasminogen activator inhibitor in the reciprocal regulation of bovine aortic endothelial and smooth muscle cell migration by TGF-beta 1. Am J Pathol 149(3):923-931
29. Powell DW, Mifflin RC, Valentich JD, Crowe SE, Saada JI, West AB (1999) Myofibroblasts. I. Paracrine cells important in health and disease. Am J Physiol 277:C1-C19
30. 1 in quiescent human breast gland fibroblasts. Implications for myofibroblast generation in breast neoplasis. Lab Invest 68:696-707
31. Saito T, Itoh H, Chun T, Igaki T, Mori Y, Yamashita J, Doi K, Tanaka T, Inoue M, Masatsugu K, Fukunaga Y, Sawada N, Tojo K, Saito Y, Hosoya T, Nakao K (1998) Oxidative stress suppresses the endothelial secretion of endothelin. J Cardiovasc Pharmacol 31(Suppl 1):345-347
32. Sakuda H, Nakashima Y, Kuriyama S, Sueishi K (1992) Media conditioned by smooth muscle cells cultured in a variety of hypoxic environments stimulates in vitro angiogenesis. A relationship to transforming growth factor-beta 1. Am J Pathol 141:1507-1516
33. Sankar S, Mahooti-Brooks N, Bensen L, McCarthy TL, Centrella M, Madri JA (1996) Modulation of transforming growth factor beta receptor levels on microvascular endothelial cells during in vitro angiogenesis. J Clin Invest 97(6):1436-1446
34. Serini G, Bochaton-Piallat ML, Ropraz P, Geinoz A, Borsi L, Zardi L, Gabbiani G (1998) The fibronectin domain ED-A is crucial for myofibroblastic phenotype induction by transforming growth factor-beta1. J Cell Biol 142(3):873-881
35. Serini G, Gabbiani G (1999) Mechanisms of myofibroblast activity and phenotypic modulation. Exp Cell Res 250:273-283
36. Shephard P, Hinz B, Smola-Hess S, Meister JJ, Krieg T, Smola H (2004) Dissecting the roles of endothelin, TGF-ß and GM-CSF on myofibroblast differentiation by keratinocytes. Thromb Haemost 92:262-274
37. Shi-Wen X, Chen Y, Denton CP, Eastwood M, Renzoni EA, Bou-Gharios G, Pearson JD, Dashwood M, du Bois RM, Black CM, Leask A, Abraham DJ (2004) Endothelin-1 promotes myofibroblast induction through the ETA receptor via a rac/phosphoinositide 3-kinase/akt-dependent pathway and is essential for the enhanced contractile phenotype of fibrotic fibroblasts. Mol Biol Cell 15:2707-2719
38. Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G (1986) A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 103:2787-2796
39. Sun G, Stacey M, Bellini A, Marini M, Mattoli S (1997) Endothelin-1 induces bronchial myofibroblast differentiation. Peptides 18(9):1449-1451
40. Thiemermann C, Corder R (1992) Is endothelin-1 the regulator of myofibroblast contraction during wound healing?. Lab Invest 67(6):667-669
41. Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA (2002) Myofibroblasts and mechanoregulation of connective tissue remodelling. Mol Cell Biol 3:349-363
42. Trabold O, Wagner S, Wicke C, Scheuenstuhl H, Hussain Z, Rosen N, Seremetiev A, Becker HD, Hunt TK (2003) Lactate and oxygen constitute a fundamental regulatory mechanism in wound healing. Wound Rep Reg 11:504-509
43. Zhao L, Eghbali-Webb M (2001) Release of pro- and anti-angiogenic factors by human cardiac fibroblasts: effects on DNA synthesis and protection under hypoxia in human endothelial cells. Biochim Biophys Acta 1538(2-3):273-82