Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Volumn 81, Issue 1, 2007, Pages 153-161

  Heinrich, Laurence ^a   Freyria, Anne Marie ^b   Melin, Martine ^a   Tourneur, Yves ^a   Maksoud, Rami ^a   Bernengo, Jean Claude ^c   Hartmann, Daniel Jean ^a  

^a UNIVERSITÉ LYON 1   (France)

^b INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

^c NII Bioparc ^*  (France)

Author keywords

Ceramic; Chondrocyte; Collagen sponge; Confocal microscopy; Dialkylcarbocyanine dyes; Fibroblast

Indexed keywords

BIOMATERIALS; BONE; CERAMIC MATERIALS; CYTOLOGY; DYES; LIGHT SCATTERING; TISSUE ENGINEERING;

CHONDROCYTE; COLLAGEN SPONGE; DIALKYLCARBOCYANINE DYES;

CONFOCAL MICROSCOPY;

ALKYL GROUP; BIOMATERIAL; CARBOCYANINE; COLLAGEN SPONGE; DYE; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; BONE REMODELING; CARTILAGE CELL; CELL LABELING; CELLULAR DISTRIBUTION; CERAMICS; CONFOCAL LASER MICROSCOPY; CONFOCAL MICROSCOPY; COW; DIFFUSION; FIBROBLAST; HUMAN; HUMAN CELL; IN VITRO STUDY; LIGHT SCATTERING; MEASUREMENT; NONHUMAN; POROSITY; TISSUE ENGINEERING;

ANIMALS; BIOCOMPATIBLE MATERIALS; BONE SUBSTITUTES; CARBOCYANINES; CARTILAGE; CATTLE; CERAMICS; CHONDROCYTES; COLLAGEN; FIBROBLASTS; FLUORESCENT DYES; HARDNESS; HUMANS; MATERIALS TESTING; MICROSCOPY, CONFOCAL;

EID: 33947672846     PISSN: 15524973     EISSN: 15524981     Source Type: Journal    
DOI: 10.1002/jbm.b.30648     Document Type: Article

References (30)

1. Logeart-Avramoglou D, Anagnostou F, Bizios R, Petite H. Engineering bone challenges and obstacles. J Cell Mol Med 2005;9:72-84.
2. Galois L, Freyria AM, Grossin L, Hubert P, Mainard D, Herbage D, Stoltz JF, Netter P, Dellacherie E, Payan E. Cartilage repair: Surgical techniques and tissue engineering using polysaccharide- and collagen-based biomaterials. Biorheology 2004; 41:433-443.
3. Uchida A, Nade SML, McCartney ER, Ching W. The use of ceramics for bone replacement. A comparative study of three different porous ceramics. J Bone Joint Surg Br 1984;66:269-275.
4. Yuan H, Kurashina K, de Bruijn JD, Li Y, de Groot K, Zhang X. A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics. Biomaterials 1999;20:1799-1806.
5. Gauthier O, Bouler J-M, Aguado E, Pilet P, Daculsi G. Macroporous biphasic calcium phosphate ceramics: Influence of macropore diameter and macroporosity percentage on bone ingrowth. Biomaterials 1998;19:133-139.
6. Ripamonti U. Osteoinduction in porous hydroxyapatite implanted in heterotopic sites of different animal models. Biomaterials 1996;17:31-35.
7. Brittberg M, Peterson L, Sjogren-Jansson E, Tallheden T, Lindahl A. Articular cartilage engineering with autologous chondrocyte transplantation. A review of recent developments. J Bone Joint Surg Am 2003;85(Suppl 3):109-115.
8. Okumura M, Ohgushi H, Dohi Y, Katuda T, Tamai S, Koerten HK, Tabata S. Osteoblastic phenotype expression on the surface of hydroxyapatite ceramics. J Biomed Mater Res 1997;37:122-129.
9. Yuan H, Li Y, de Bruijn JD, de Groot K, Zhang X. Tissue responses of calcium phosphate cement: A study in dogs. Biomaterials 2000;21:1283-1290.
10. Bignon A, Chouteau J, Chevalier J, Fantozzi G, Garret J-P, Chavassieu P, Boivin G, Melin M, Hartmann D. Effects of micro- and macroporosity of bone substitutes on their mechanical properties and cellular response. J Mater Sci Mater Med 2003;14:1089-1097.
11. Tearney GJ, Brezinski ME, Bouma BE, Boppart SA, Pitris C, Southern JF, Fujimoto JG. In vivo endoscopic optical biopsy with optical coherence tomography. Science 1997;276:2037-2039.
12. Gauthier O, Muller R, von Stechow D, Lamy B, Weiss P, Bouler JM, Aguado E, Daculsi G. In vivo bone regeneration with injectable calcium phosphate biomaterial: A three-dimensional micro-computed tomographic, biomechanical and SEM study. Biomaterials 2005;26:5444-5453.
13. Hanthamrongwit M, Wilkinson R, Osborne C, Reid WH, Grant MH. Confocal laser-scanning microscopy for determining the structure of and keratinocyte infiltration through collagen sponges. J Biomed Mater Res 1996;30:331-339.
14. Ramires PA, Giuffrida A, Milella E. Three-dimensional reconstruction of confocal laser microscopy images to study the behavior of osteoblastic cells grown on biomaterials. Biomaterials 2002;23:397-406.
15. Walboomers XF, Croes HJE, Ginsel LA, Jansen JA. Growth behavior of fibroblasts on microgrooved polystyrene. Biomaterials 1998;19:1861-1868.
16. Monaghan P, Watson PR, Cook H, Scott L, Wallis TS, Robertson D. An improved method for preparing thick sections for immuno/histochemistry and confocal microscopy and its use to identify rare events. J Microsc 2001;203:223-226.
17. Mueller-Stahl K, Kofidis T, Akhyari P, Wachsmann B, Lenz A, Boublik J, Heine M, Muehlfait V, Haverich A, Mertsching H. Carboxyfluorescein diacetate succinimidyl ester facilitates cell tracing and colocalization studies in bioartificial organ engineering. Int J Artif Organs 2003;26:235-240.
18. Honig MG, Hume RI. Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J Cell Biol 1986;103:171-187.
19. De Clerck LS, Bridts CH, Mertens AM, Moens MM, Stevens WJ. Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function. J Immunol Methods 1994;172:115-124.
20. Ferrari A, Hannouche D, Oudina K, Bourguignon M, Meunier A, Sedel L, Petite H. In vivo tracking of bone marrow fibroblasts with fluorescent carbocyanine dye. J Biomed Mater Res 2001;56:361-367.
21. Schopper C, Ziya-Ghazvini F, Goriwoda W, Moser D, Wanschitz F, Spassova E, Lagogiannis G, Auterith A, Ewers R. HA/TCP compounding of a porous CaP biomaterial improves bone formation and scaffold degradation-A long term histological study. J Biomed Mater Res B Appl Biomater 2005;74:458-467.
22. Silver FH, Pins G. Cell growth on collagen: A review of tissue engineering using scaffolds containing extracellular matrix. J Long Term Eff Med Implants 1992;2:67-80.
23. Woodfield TB, Bezemer JM, Pieper JS, van Blitterswijk CA, Riesle J. Scaffolds for tissue engineering of cartilage. Crit Rev Eukaryot Gene Expr 2002;12:209-236.
24. Ronzière M-C, Roche S, Gouttenoire J, Démarteau O, Herbage D, Freyria A-M. Ascorbate modulation of bovine chondrocyte growth, matrix protein gene expression and syntheses in three-dimensional collagen sponges. Biomaterials 2003;24:851-861.
25. Cullander C. Imaging in the far-red with electronic light microscopy: Requirements and limitations. J Microsc 1994;176:281-286.
26. Roche S, Ronzière M-C, Herbage D, Freyria A-M. Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. Biomaterials 2001;22:9-18.
27. Pieper JS, Hafmans T, Veerkamp JH, van Kuppevelt TH. Development of tailor-made collagen-glycosaminoglycan matrices: EDC/NHS crosslinking, and ultrastructural aspects. Biomaterials 2000;21:581-593.
28. Freyria A-M, Cortial D, Ronzière M-C, Guerret S, Herbage D. Influence of medium composition, static and stirred conditions on the proliferation of and matrix protein expression of bovine articular chondrocytes cultured in a 3-D collagen scaffold. Biomaterials 2004;25:687-697.
29. Denk W, Strickler J, Webb WW. Two photon laser scanning microscopy. Science 1990;248:73-76.
30. Kirchhof K, Grohmann S, Schade R, Liefeith K, Mollenhauer J. Comparative microscopic and biochemical investigations on the ECM synthesis of chondrocytes cultured on specific scaffold materials. Presented at the Fourth Annual Meeting of the European Tissue Engineering Society, Munich, Germany, 2005 (Abstract No. 341).