Human growth hormone: 45-kDa isoform with extraordinarily stable interchain disulfide links has attenuated receptor-binding and cell-proliferative activities

Growth Hormone and IGF Research

Volumn 20, Issue 4, 2010, Pages 298-304

  Bustamante, Juan J ^a   Grigorian, Alexei L ^b   Muñoz, Jesus ^c   Aguilar, Roberto M ^d   Treviño, Lisa R ^e   Martinez, Andrew O ^c   Haro, Luis S ^c  

^a TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

^b UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF TEXAS AT SAN ANTONIO   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

Author keywords

Dimeric human growth hormone; Isoform; Molecular variant; Radioreceptor assay; Somatotropin

Indexed keywords

GROWTH HORMONE; GROWTH HORMONE RECEPTOR; HUMAN GROWTH HORMONE; MERCAPTOETHANOL; PROLACTIN; PROLACTIN RECEPTOR;

ARTICLE; BINDING AFFINITY; BINDING COMPETITION; CANCER CELL; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DIMERIZATION; DISULFIDE BOND; HUMAN; HUMAN CELL; LIVER MEMBRANE; LYMPHOMA CELL; MAMMARY GLAND; PRIORITY JOURNAL; RABBIT; RECEPTOR BINDING;

ANIMALS; BINDING SITES; CATTLE; CELL LINE, TUMOR; CELL PROLIFERATION; FEMALE; HUMAN GROWTH HORMONE; HUMANS; LYMPHOCYTES; PROLACTIN; PROTEIN ISOFORMS; RABBITS; RATS; RECEPTORS, PROLACTIN;

EID: 77955422848     PISSN: 10966374     EISSN: 15322238     Source Type: Journal    
DOI: 10.1016/j.ghir.2010.03.007     Document Type: Article

References (48)

1. Sanders E.J., Harvey S. Growth hormone as an early embryonic growth and differentiation factor. Anat. Embryol. (Berl) 2004, 209:1-9.
2. Rennie M.J. Claims for the anabolic effects of growth hormone: a case of the emperor's new clothes?. Br. J. Sports Med. 2003, 37:100-105.
3. Kelly P.A., Bachelot A., Kedzia C., et al. The role of prolactin and growth hormone in mammary gland development. Mol. Cell. Endocrinol. 2002, 197:127-131.
4. Singh R.N., Seavey B.K., Lewis U.J. Heterogeneity of human growth hormone. Endocr. Res. Commun. 1974, 1:449-464.
5. Lewis U.J., Singh R.N., Tutwiler G.F., Sigel M.B., VanderLaan E.F., VanderLaan W.P. Human growth hormone: a complex of proteins. Recent Prog. Horm. Res. 1980, 36:477-508.
6. Baumann G. Growth hormone heterogeneity: genes, isohormones, variants, and binding proteins. Endocr. Rev. 1991, 12:424-449.
7. Lewis U.J. Growth hormone: what is it and what does it do?. Trends Endocrinol. Metab. 1992, 3:117-121.
8. Baumann G. Growth hormone heterogeneity in human pituitary and plasma. Horm. Res. 1999, 51(Suppl. 1):2-6.
9. Baumann G.P. Growth hormone isoforms, Growth Horm. IGF Res. 2009, 19:333-340.
10. Lewis U.J., Sinha Y.N., Haro L.S. Variant forms and fragments of human growth hormone in serum. Acta Paediatr. Suppl. 1994, 399:29-31. (discussion 32.).
11. Bustamante J.J., Gonzalez L., Carroll C.A., et al. O-Glycosylated 24kDa human growth hormone has a mucin-like biantennary disialylated tetrasaccharide attached at Thr-60. Proteomics 2009, 9:3474-3488.
12. Haro L.S., Lewis U.J., Garcia M., Bustamante J., Martinez A.O., Ling N.C. Glycosylated human growth hormone (hGH): a novel 24kDa hGH-N variant. Biochem. Biophys. Res. Commun. 1996, 228:549-556.
13. Diaz M.J., Dominguez F., Haro L.S., Ling N., Devesa J. A 12-kilodalton N-glycosylated growth hormone-related peptide is present in human pituitary extracts. J. Clin. Endocrinol. Metab. 1993, 77:134-138.
14. Lewis U.J., Singh R.N., Bonewald L.F., Seavey B.K. Altered proteolytic cleavage of human growth hormone as a result of deamidation. J. Biol. Chem. 1981, 256:11645-11650.
15. Giorgianni F., Beranova-Giorgianni S., Desiderio D.M. Identification and characterization of phosphorylated proteins in the human pituitary. Proteomics 2004, 4:587-598.
16. Zhan X., Giorgianni F., Desiderio D.M. Proteomics analysis of growth hormone isoforms in the human pituitary. Proteomics 2005, 5:1228-1241.
17. Nindl B.C., Kraemer W.J., Marx J.O., Tuckow A.P., Hymer W.C. Growth hormone molecular heterogeneity and exercise. Exerc. Sport Sci. Rev. 2003, 31:161-166.
18. Grigorian A.L., Bustamante J.J., Hernandez P., Martinez A.O., Haro L.S. Extraordinarily stable disulfide-linked homodimer of human growth hormone. Protein Sci. 2005, 14:902-913.
19. Grigorian A.L., Bustamante J.J., Munoz J., Aguilar R.M., Martinez A.O., Haro L.S. Preparative alkaline urea gradient PAGE: application to purification of extraordinarily-stable disulfide-linked homodimer of human growth hormone. Electrophoresis 2007, 28:3829-3836.
20. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
21. Salacinski P.R., McLean C., Sykes J.E., Clement-Jones V.V., Lowry P.J. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1, 3, 4, 6-tetrachloro-3 alpha, 6 alpha-diphenyl glycoluril (Iodogen). Anal. Biochem. 1981, 117:136-146.
22. Haro L.S., Talamantes F.J. Secreted mouse prolactin (PRL) and stored ovine PRL. II. Role of amides in receptor binding and immunoreactivity. Endocrinology 1985, 116:353-358.
23. Haro L.S., Collier R.J., Talamantes F.J. Homologous somatotropin radioreceptor assay utilizing recombinant bovine growth hormone. Mol. Cell. Endocrinol. 1984, 38:109-116.
24. DeLean A., Munson P.J., Rodbard D. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Am. J. Physiol. 1978, 235:E97-102.
25. Lesniak M.A., Roth J. Regulation of receptor concentration by homologous hormone. Effect of human growth hormone on its receptor in IM-9 lymphocytes. J. Biol. Chem. 1976, 251:3720-3729.
26. Ohmae Y., Yano H., Umezawa S., et al. Biological activities of synthesized 20K and 22K hGH in Nb2 bioassay and IM-9 radioreceptor assay. Endocrinol. Jpn. 1989, 36:9-13.
27. Tanaka T., Shiu R.P., Gout P.W., Beer C.T., Noble R.L., Friesen H.G. A new sensitive and specific bioassay for lactogenic hormones: measurement of prolactin and growth hormone in human serum. J. Clin. Endocrinol. Metab. 1980, 51:1058-1063.
28. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65:55-63.
29. Ginsburg E., Vonderhaar B.K. Prolactin synthesis and secretion by human breast cancer cells. Cancer Res. 1995, 55:2591-2595.
30. Freshney R.I. Culture of Animal Cells: a Manual of Basic Technique 1987, Wiley-Liss, Inc., New York. 2nd Edition.
31. Lewis U.J., Peterson S.M., Bonewald L.F., Seavey B.K., VanderLaan W.P. An interchain disulfide dimer of human growth hormone. J. Biol. Chem. 1977, 252:3697-3702.
32. Talamantes F., Lopez J., Lewis U.J., Wilson C.B. Multiple forms of growth hormone: detection in medium from cultured pituitary adenoma explants. Acta Endocrinol. (Copenh) 1981, 98:8-13.
33. Lewis U.J., Singh R.N.P., Lewis L.J., Abadi N. Detection of multiple forms of human growth hormone. Acromegaly: a Century of Scientific and Clinical Progress 1987, 27-34. Plenum, New York. R.J. Robbins, S. Melmed (Eds.).
34. Talamantes F., Ogren L., Markoff E., Woodard S., Madrid J. Phylogenetic distribution, regulation of secretion, and prolactin-like effects of placental lactogens. Fed. Proc. 1980, 39:2582-2587.
35. Forsyth I.A., Wallis M. Growth hormone and prolactin-molecular and functional evolution. J. Mammary Gland Biol. Neoplasia 2002, 7:291-312.
36. Soares M.J. The prolactin and growth hormone families: pregnancy-specific hormones/cytokines at the maternal-fetal interface. Reprod. Biol. Endocrinol. 2004, 2:51.
37. Nicoll C.S. Prolactin and growth hormone: specialists on one hand and mutual mimics on the other. Perspect. Biol. Med. 1982, 25:369-381.
38. Nicoll C.S., Mayer G.L., Russell S.M. Structural features of prolactins and growth hormones that can be related to their biological properties. Endocr. Rev. 1986, 7:169-203.
39. Oppezzo O.J., Biscoglio de Jimenez Bonino M., Cascone O., et al. Hydrophobic residues involved in the interaction between protomers of the bovine growth hormone dimer. Methionine and tyrosine residues. Acta Physiol. Pharmacol. Latinoam. 1984, 34:175-184.
40. Oppezzo O.J., Fernandez H.N. Contact area of bovine somatotropin dimer: involvement of tyrosine 142. Int. J. Pept. Protein Res. 1991, 37:277-282.
41. Mockridge J.W., Aston R., Morrell D.J., Holder A.T. Cross-linked growth hormone dimers have enhanced biological activity. Eur. J. Endocrinol. 1998, 138:449-459.
42. Langenheim J.F., Tan D., Walker A.M., Chen W.Y. Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Mol. Endocrinol. 2006, 20:661-674.
43. Violand B.N., Takano M., Curran D.F., Bentle L.A. A novel concatenated dimer of recombinant bovine somatotropin. J. Protein Chem. 1989, 8:619-628.
44. Yang N., Langenheim J.F., Wang X., Jiang J., Chen W.Y., Frank S.J. Activation of growth hormone receptors by growth hormone and growth hormone antagonist dimers: insights into receptor triggering. Mol. Endocrinol. 2008, 22:978-988.
45. Becker G.W., Bowsher R.R., Mackellar W.C., Poor M.L., Tackitt P.M., Riggin R.M. Chemical, physical, and biological characterization of a dimeric form of biosynthetic human growth hormone. Biotechnol. Appl. Biochem. 1987, 9:478-487.
46. Brostedt P., Luthman M., Wide L., Werner S., Roos P. Characterization of dimeric forms of human pituitary growth hormone by bioassay, radioreceptor assay, and radioimmunoassay. Acta Endocrinol. (Copenh) 1990, 122:241-248.
47. Brostedt P., Roos P. Isolation of dimeric forms of human pituitary growth hormone. Prep. Biochem. 1989, 19:217-229.
48. Fernandez H.N., Delfino J.M. Covalent cross-linking of the bovine somatotropin dimer. Effects on growth-promoting, receptor-binding and immunological activities and preliminary characterization of the self-association. Biochem. J. 1983, 209:107-115.