Preparative alkaline urea gradient PAGE: Application to purification of extraordinarily-stable disulfide-linked homodimer of human growth hormone

Electrophoresis

Volumn 28, Issue 21, 2007, Pages 3829-3836

  Grigorian, Alexei L ^a   Bustamante, Juan J ^b   Muñoz, Jesus ^c   Aguilar, Roberto M ^d   Martinez, Andrew O ^c   Haro, Luis S ^c  

^a UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF TEXAS AT SAN ANTONIO   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cytokine; Disulfide dimer; Human growth hormone; Preparative PAGE; Radioimmunoassay

Indexed keywords

ALKALINITY; DEXTRAN; DIMERS; ELECTROPHORESIS; ION EXCHANGE; LIQUID CHROMATOGRAPHY; METABOLISM; PROTEINS; SOLS; UREA;

ALKALINES; CYTOKINES; DISULPHIDE DIMER; HUMAN GROWTH HORMONE; ISOFORMS; MERCAPTOETHANOL; PREPARATIVE PAGE; RADIOIMMUNOASSAY; SEPHADEX; UREA GRADIENT;

SULFUR COMPOUNDS;

2 DIETHYLAMINOETHANOL; HOMODIMER; HUMAN GROWTH HORMONE; HYPOPHYSIS EXTRACT; MERCAPTOETHANOL; MONOMER; UREA;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISULFIDE BOND; HUMAN; HYDROPHOBICITY; IMMUNOREACTIVITY; METHODOLOGY; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN FAMILY; PROTEIN STRUCTURE; RADIOIMMUNOASSAY; SEPARATION TECHNIQUE;

EID: 36349019730     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200700201     Document Type: Article

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