Moisture-induced solid state instabilities in α-chymotrypsin and their reduction through chemical glycosylation

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Flores Fernández, Giselle M ^a   Pagán, Miraida ^a   Almenas, Mariangely ^a   Solá, Ricardo J ^a   Griebenow, Kai ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCANS; GLYCOCONJUGATES; H/D EXCHANGE; HUMIDITY CONDITIONS; INSTABILITY PROBLEMS; MODEL PROTEINS; MOISTURE EXPOSURE; MOISTURE SORPTION; NATIVE CONFORMATION; NOVEL METHODOLOGY; OPERATIONAL COSTS; PROTEIN FORMULATION; PROTEIN GLYCOSYLATION; PROTEIN INSTABILITY; PROTEIN SURFACE; PROTEIN THERAPEUTICS; RELATIVE HUMIDITIES; RESIDUAL ACTIVITY; SECONDARY AND TERTIARY STRUCTURES; SOLID PROTEIN; STABILITY PROPERTIES; STABILIZATION EFFECTS; STERIC HINDRANCES; STRUCTURAL CHANGE; STRUCTURAL CONFORMATIONS; STRUCTURAL PERTURBATION; WATER MOLECULE; WATER SORPTION;

ATMOSPHERIC HUMIDITY; CARBOHYDRATES; CHEMICAL STABILITY; CONFORMATIONS; DRUG PRODUCTS; DYES; ESTERIFICATION; GLUCOSE; GLYCOSYLATION; HUMIDITY CONTROL; MOISTURE; SORPTION; STABILIZATION; STRUCTURAL DYNAMICS; SUGAR (SUCROSE);

PROTEINS;

CHYMOTRYPSIN A; DEXTRAN; LACTOSE; ALPHA-CHYMOTRYPSIN; CHYMOTRYPSIN; GLYCOCONJUGATE; WATER;

ARTICLE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME INACTIVATION; ENZYME KINETICS; FREEZE DRYING; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; HUMIDITY; MOISTURE; PROTEIN AGGREGATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; SOLID STATE; CHEMISTRY; ENZYME STABILITY; KINETICS; SYNTHESIS;

CHYMOTRYPSIN; DEXTRANS; ENZYME STABILITY; FREEZE DRYING; GLYCOCONJUGATES; GLYCOSYLATION; HUMIDITY; KINETICS; WATER;

EID: 77955286460     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-57     Document Type: Article

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