Mgat1-dependent N-glycans are essential for the normal development of both vertebrate and invertebrate metazoans

Seminars in Cell and Developmental Biology

Volumn 21, Issue 6, 2010, Pages 609-615

  Schachter, Harry ^a  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Caenorhabditis; Development; Drosophila; Mouse; N Acetylglucosaminyltransferase I

Indexed keywords

ALPHA1,6 FUCOSYLTRANSFERASE; FUCOSYLTRANSFERASE; GLYCAN DERIVATIVE; MANNOSE; MANNOSE OLIGOSACCHARIDE; N ACETYLGLUCOSAMINYLTRANSFERASE; N GLYCAN DERIVATIVE; PAUCIMANNOSE; PHOSPHORYLCHOLINE; UNCLASSIFIED DRUG;

CAENORHABDITIS ELEGANS; DROSOPHILA MELANOGASTER; FUCOSYLATION; GENE; GENE MUTATION; GENE STRUCTURE; INVERTEBRATE; METAZOON; MGAT1 GENE; NONHUMAN; PROTEIN SYNTHESIS; REVIEW; VERTEBRATE;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; DROSOPHILA MELANOGASTER; INVERTEBRATA; METAZOA; MUS; VERTEBRATA;

EID: 77955277390     PISSN: 10849521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.semcdb.2010.02.010     Document Type: Review

References (96)

1. Freeze H.H. Genetic defects in the human glycome. Nat Rev Genet 2006, 7(7):537-551.
2. Henrissat B., Surolia A., Stanley P. A genomic view of glycobiology. Essentials of glycobiology 2008, 89-99. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, [Chapter 7]. 2nd ed. A. Varki, R.D. Cummings, J.D. Esko, H.H. Freeze, P. Stanley, C.R. Bertozzi, G.W. Hart, M.E. Etzler (Eds.).
3. Narimatsu H. Human glycogene cloning: focus on beta 3-glycosyltransferase and beta 4-glycosyltransferase families. Curr Opin Struct Biol 2006, 16(5):567-575.
4. Krishna R.G., Wold F. Post-translational modification of proteins. Adv Enzymol Relat Areas Mol Biol 1993, 67:265-298.
5. Lowe J.B., Marth J.D. A genetic approach to mammalian glycan function. Annu Rev Biochem 2003, 72:643-691.
6. Varki A., Lowe J.B. Biological roles of glycans. Essentials of glycobiology 2008, 75-88. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, [chapter 6]. 2nd ed. A. Varki, R.D. Cummings, J.D. Esko, H.H. Freeze, P. Stanley, C.R. Bertozzi, G.W. Hart, M.E. Etzler (Eds.).
7. Freeze H.H., Schachter H. Genetic disorders of glycosylation. Essentials of glycobiology 2008, 585-600. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, [chapter 42]. 2nd ed. A. Varki, R.D. Cummings, J.D. Esko, H.H. Freeze, P. Stanley, C.R. Bertozzi, G.W. Hart, M.E. Etzler (Eds.).
8. Varki A., Freeze H., Gagneux P. Evolution of glycan diversity. Essentials of glycobiology 2008, 281-292. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, [chapter 19]. 2nd ed. A. Varki, R.D. Cummings, J.D. Esko, H.H. Freeze, P. Stanley, C.R. Bertozzi, G.W. Hart, M.E. Etzler (Eds.).
9. Spiro R.G. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology 2002, 12(4):43R-56R.
10. Schachter H. The 'yellow brick road' to branched complex N-glycans. Glycobiology 1991, 1(5):453-461.
11. Schachter H. The joys of HexNAc. The synthesis and function of N- and O-glycan branches. Glycoconj J 2000, 17(7-9):465-483.
12. Hemming F.W. Biosynthesis. The coenzymic role of phosphodolichols. Glycoproteins, new comprehensive biochemistry 1995, vol. 29a:127-143. Elsevier, Amsterdam, The Netherlands.
13. Verbert A. Biosynthesis. From Glc3Man9GlcNAc2-protein to Man5GlcNAc2-prote: transfer 'en bloc' and processing. Glycoproteins, new comprehensive biochemistry 1995, vol. 29a:145-152. Elsevier, Amsterdam, The Netherlands.
14. Burda P., Aebi M. The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta-Gen Subj 1999, 1426(2):239-257.
15. Spiro R.G. Role of N-linked polymannose oligosaccharides in targeting glycoproteins for endoplasmic reticulum-associated degradation. Cell Mol Life Sci 2004, 61(9):1025-1041.
16. Herscovics A. Importance of glycosidases in mammalian glycoprotein biosynthesis. Biochim Biophys Acta 1999, 1473(1):96-107.
17. Moremen K.W. Golgi alpha-mannosidase II deficiency in vertebrate systems: implications for asparagine-linked oligosaccharide processing in mammals. Biochim Biophys Acta 2002, 1573(3):225-235.
18. Schachter H. Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides. Biochem Cell Biol 1986, 64(3):163-181.
19. Schachter H. Biosynthesis. Glycosyltransferases involved in the synthesis of N-glycan antennae. Glycoproteins, new comprehensive biochemistry 1995, vol. 29a:153-199. Elsevier, Amsterdam, The Netherlands.
20. Vitale A., Chrispeels M.J. Transient N-acetylglucosamine in the biosynthesis of phytohemagglutinin: attachment in the Golgi apparatus and removal in protein bodies. J Cell Biol 1984, 99:133-140.
21. Zhang W., Cao P., Chen S., Spence A.M., Zhu S., Staudacher E., et al. Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-GlcNAc:alpha-3-d-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha-3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase. Biochem J 2003, 372(1):53-64.
22. Sarkar M., Leventis P.A., Silvescu C.I., Reinhold V.N., Schachter H., Boulianne G.L. Null mutations in Drosophila N-acetylglucosaminyltransferase I produce defects in locomotion and a reduced lifespan. J Biol Chem 2006, 281(18):12776-12785.
23. Altmann F., Schwihla H., Staudacher E., Glossl J., Marz L. Insect cells contain an unusual, membrane-bound beta-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J Biol Chem 1995, 270(29):17344-17349.
24. Leonard R., Rendic D., Rabouille C., Wilson I.B., Preat T., Altmann F. The Drosophila fused lobes gene encodes an N-acetylglucosaminidase involved in N-glycan processing. J Biol Chem 2006, 281(8):4867-4875.
25. Schachter H. Processing of protein-bound N-glycans. Comprehensive glycoscience: from chemistry to systems biology 2007, vol. 3:11-32. Elsevier, Oxford, UK.
26. Gottlieb C., Baenziger J., Kornfeld S. Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins. J Biol Chem 1975, 250(9):3303-3309.
27. Stanley P., Narasimhan S., Siminovitch L., Schachter H. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine: glycoprotein N-acetylglucosaminyltransferase activity. Proc Nat Acad Sci (USA) 1975, 72(9):3323-3327.
28. Vischer P., Hughes R.C. Glycosyl transferases of baby-hamster-kidney (BHK) cells and ricin-resistant mutants. N-Glycan biosynthesis. Eur J Biochem 1981, 117(2):275-284.
29. Ioffe E., Stanley P. Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates. Proc Natl Acad Sci USA 1994, 91(2):728-732.
30. Metzler M., Gertz A., Sarkar M., Schachter H., Schrader J.W., Marth J.D. Complex asparagine-linked oligosaccharides are required for morphogenic events during post-implantation development. EMBO J 1994, 13(9):2056-2065.
31. Charuk J.H.M., Tan J., Bernardini M., Haddad S., Reithmeier R.A.F., Jaeken J., et al. Carbohydrate-deficient glycoprotein syndrome type II-an autosomal recessive N-acetylglucosaminyltransferase II deficiency different from typical hereditary erythroblastic multinuclearity, with a positive acidified-serum lysis test (HEMPAS). Eur J Biochem 1995, 230(2):797-805.
32. Jaeken J., Schachter H., Carchon H., De Cock P., Coddeville B., Spik G. Carbohydrate deficient glycoprotein syndrome type II: a deficiency in Golgi localised N-acetylglucosaminyltransferase II. Arch Dis Child 1994, 71(2):123-127.
33. Tan J., Dunn J., Jaeken J., Schachter H. Mutations in the MGAT2 gene controlling complex N-glycan synthesis cause carbohydrate-deficient glycoprotein syndrome type II, an autosomal recessive disease with defective brain development. Am J Hum Genet 1996, 59(4):810-817.
34. Wang Y., Schachter H., Marth J.D. Mice with a homozygous deletion of the Mgat2 gene encoding UDP-N-acetylglucosamine:alpha-6-d-mannoside beta1,2-N-acetylglucosaminyltransferase II: a model for congenital disorder of glycosylation type IIa. Biochim Biophys Acta 2002, 1573(3):301-311.
35. Wang Y., Tan J., Sutton-Smith M., Ditto D., Panico M., Campbell R.M., et al. Modeling human congenital disorder of glycosylation type IIa in the mouse: conservation of asparagine-linked glycan-dependent functions in mammalian physiology and insights into disease pathogenesis. Glycobiology 2001, 11(12):1051-1070.
36. Zhu S., Hanneman A., Reinhold V.N., Spence A.M., Schachter H. Caenorhabditis elegans triple null mutant lacking UDP-N-acetyl-d-glucosamine:alpha-3-d-mannoside beta1,2-N-acetylglucosaminyltransferase I. Biochem J 2004, 382(Pt 3):995-1001.
37. Shi H., Tan J., Schachter H. N-glycans are involved in the response of Caenorhabditis elegans to bacterial pathogens. Methods Enzymol 2006, 417:359-389.
38. Dennis J.W., Nabi I.R., Demetriou M. Metabolism, cell surface organization, and disease. Cell 2009, 139(7):1229-1241.
39. Schachter H. Protein glycosylation lessons from Caenorhabditis elegans. Curr Opin Struct Biol 2004, 14(5):607-616.
40. Schachter H. Glycobiology of Caenorhabditis elegans. Comprehensive glycoscience - from chemistry to systems biology 2007, vol. 4:81-100. Elsevier, Amsterdam.
41. Chen S.H., Zhou S.H., Sarkar M., Spence A.M., Schachter H. Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase I genes during development. J Biol Chem 1999, 274(1):288-297.
42. Garsin D.A., Sifri C.D., Mylonakis E., Qin X., Singh K.V., Murray B.E., et al. A simple model host for identifying Gram-positive virulence factors. Proc Natl Acad Sci USA 2001, 98(19):10892-10897.
43. Alegado R.A., Campbell M.C., Chen W.C., Slutz S.S., Tan M.W. Characterization of mediators of microbial virulence and innate immunity using the Caenorhabditis elegans host-pathogen model. Cell Microbiol 2003, 5(7):435-444.
44. Sarkar M., Schachter H. Cloning and expression of Drosophila melanogaster UDP-GlcNAc:alpha-3-d-mannoside beta1,2-N-acetylglucosaminyltransferase I. Biol Chem 2001, 382(2):209-217.
45. Schachter H., Sarkar M., Leventis P.A., Iliadi K., Boulianne G.L. Neuronal expression of Mgat1 rescues the Mgat1-null mutant phenotype and dramatically increases Drosophila lifespan. Glycobiology 2009, 19(11):1295.
46. Lee T., Lee A., Luo L. Development of the Drosophila mushroom bodies: sequential generation of three distinct types of neurons from a neuroblast. Development 1999, 126(18):4065-4076.
47. Lin W.Y. NMDA receptors are required in memory formation in Drosophila mushroom body. Biochem Biophys Res Commun 2005, 334(3):779-786.
48. Zhao X., Coptis V., Farris S.M. Metamorphosis and adult development of the mushroom bodies of the red flour beetle, Tribolium castaneum. Dev Neurobiol 2008, 68(13):1487-1502.
49. Tanaka N.K., Tanimoto H., Ito K. Neuronal assemblies of the Drosophila mushroom body. J Comp Neurol 2008, 508(5):711-755.
50. Paschinger K., Gutternigg M., Rendic D., Wilson I.B. The N-glycosylation pattern of Caenorhabditis elegans. Carbohydr Res 2008, 343(12):2041-2049.
51. Paschinger K., Staudacher E., Stemmer U., Fabini G., Wilson I.B. Fucosyltransferase substrate specificity and the order of fucosylation in invertebrates. Glycobiology 2005, 15(5):463-474.
52. Fabini G., Freilinger A., Altmann F., Wilson I.B. Identification of core alpha1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster: potential basis of the neural anti-horseradish peroxidase epitope. J Biol Chem 2001, 276(30):28058-28067.
53. Paschinger K., Rendic D., Lochnit G., Jantsch V., Wilson I.B. Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans. J Biol Chem 2004, 279(48):49588-49598.
54. Rendic D., Linder A., Paschinger K., Borth N., Wilson I.B., Fabini G. Modulation of neural carbohydrate epitope expression in Drosophila melanogaster cells. J Biol Chem 2006, 281(6):3343-3353.
55. Grabitzki J., Ahrend M., Schachter H., Geyer R., Lochnit G. The PCome of Caenorhabditis elegans as a prototypic model system for parasitic nematodes: identification of phosphorylcholine-substituted proteins. Mol Biochem Parasitol 2008, 161(2):101-111.
56. Houston K.M., Sutharsan R., Steiger C.N., Schachter H., Harnett W. Gene inactivation confirms the identity of enzymes involved in nematode phosphorylcholine-N-glycan synthesis. Mol Biochem Parasitol 2008, 157(1):88-91.
57. Zheng Q., Van Die I., Cummings R.D. A novel alpha1,2-fucosyltransferase (CE2FT-2) in Caenorhabditis elegans generates H-type 3 glycan structures. Glycobiology 2008, 18(4):290-302.
58. Zheng Q., Van Die I., Cummings R.D. Molecular cloning and characterization of a novel alpha 1,2-fucosyltransferase (CE2FT-1) from Caenorhabditis elegans. J Biol Chem 2002, 277(42):39823-39832.
59. Nguyen K., van Die I., Grundahl K.M., Kawar Z.S., Cummings R.D. Molecular cloning and characterization of the Caenorhabditis elegans {alpha}1,3-fucosyltransferase family. Glycobiology 2007, 17:586-599.
60. DeBose-Boyd R.A., Nyame A.K., Cummings R.D. Molecular cloning and characterization of an alpha1,3 fucosyltransferase, CEFT-1, from Caenorhabditis elegans. Glycobiology 1998, 8(9):905-917.
61. van Die I., Gomord V., Kooyman F.N., van den Berg T.K., Cummings R.D., Vervelde L. Core alpha1,3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep. FEBS Lett 1999, 463(1-2):189-193.
62. Paschinger K., Rendic D., Wilson I.B. Revealing the anti-HRP epitope in Drosophila and Caenorhabditis. Glycoconj J 2009, 26(3):385-395.
63. Lochnit G., Dennis R.D., Geyer R. Phosphorylcholine substituents in nematodes: structures, occurrence and biological implications. Biol Chem 2000, 381(9-10):839-847.
64. Gerdt S., Dennis R.D., Borgonie G., Schnabel R., Geyer R. Isolation, characterization and immunolocalization of phosphorylcholine-substituted glycolipids in developmental stages of Caenorhabditis elegans. Eur J Biochem 1999, 266(3):952-963.
65. Gerdt S., Lochnit G., Dennis R.D., Geyer R. Isolation and structural analysis of three neutral glycosphingolipids from a mixed population of Caenorhabditis elegans (Nematoda: Rhabditida). Glycobiology 1997, 7(2):265-275.
66. Houston K.M., Harnett W. Structure and synthesis of nematode phosphorylcholine-containing glycoconjugates. Parasitology 2004, 129(Pt 6):655-661.
67. Lochnit G., Grabitzki J., Henkel B., Tavernarakis N., Geyer R. First identification of a phosphorylcholine-substituted protein from Caenorhabditis elegans: isolation and characterization of the aspartyl protease ASP-6. Biol Chem 2006, 387(10-11):1487-1493.
68. Haslam S.M., Khoo K.-H., Houston K.M., Harnett W., Morris H.R., Dell A. Characterisation of the phosphorylcholine-containing N-linked oligosaccharides in the excretory-secretory 62kDa glycoprotein of Acanthocheilonema viteae. Mol Biochem Parasitol 1997, 85(1):53-66.
69. Haslam S.M., Houston K.M., Harnett W., Reason A.J., Morris H.R., Dell A. Structural studies of N-glycans of filarial parasites. Conservation of phosphorylcholine-substituted glycans among species and discovery of novel chito-oligomers. J Biol Chem 1999, 274(30):20953-20960.
70. Goodridge H.S., Stepek G., Harnett W., Harnett M.M. Signalling mechanisms underlying subversion of the immune response by the filarial nematode secreted product ES-62. Immunology 2005, 115(3):296-304.
71. Harnett W., Harnett M.M. Modulation of the host immune system by phosphorylcholine-containing glycoproteins secreted by parasitic filarial nematodes. Biochim Biophys Acta 2001, 1539(1-2):7-15.
72. Harnett W., Harnett M.M. Filarial nematode secreted product ES-62 is an anti-inflammatory agent: therapeutic potential of small molecule derivatives and ES-62 peptide mimetics. Clin Exp Pharmacol Physiol 2006, 33(5-6):511-518.
73. Cipollo J.F., Awad A., Costello C.E., Robbins P.W., Hirschberg C.B. Biosynthesis in vitro of Caenorhabditis elegans phosphorylcholine oligosaccharides. Proc Natl Acad Sci USA 2004, 101(10):3404-3408.
74. Campbell R.M., Metzler M., Granovsky M., Dennis J.W., Marth J.D. Complex asparagine-linked oligosaccharides in Mgat1-null embryos. Glycobiology 1995, 5(5):535-543.
75. Ioffe E., Liu Y., Stanley P. Complex N-glycans in Mgat1 null preimplantation embryos arise from maternal Mgat1 RNA. Glycobiology 1997, 7(7):913-919.
76. Schachter H., Jaeken J. Carbohydrate-deficient glycoprotein syndrome type II. Biochim. Biophys. Acta 1999, 1455(2-3):179-192.
77. Ye Z., Marth J.D. N-glycan branching requirement in neuronal and post-natal viability. Glycobiology 2004, 14(6):547-558.
78. Priatel J.J., Sarkar M., Schachter H., Marth J.D. Isolation, characterization and inactivation of the mouse Mgat3 gene: the bisecting N-acetylglucosamine in asparagine-linked oligosaccharides appears dispensable for viability and reproduction. Glycobiology 1997, 7(1):45-56.
79. Brockhausen I., Carver J., Schachter H. Control of glycoprotein synthesis. XIV. The use of oligosaccharide substrates and HPLC to study the sequential pathway for N-acetylglucosaminyltransferases I, II, III, IV, V and VI in the biosynthesis of highly branched N-glycans by hen oviduct membranes. Biochem Cell Biol 1988, 66(10):1134-1151.
80. Bhaumik M., Harris T., Sundaram S., Johnson L., Guttenplan J., Rogler C., et al. Progression of hepatic neoplasms is severely retarded in mice lacking the bisecting N-acetylglucosamine on N-glycans: Evidence for a glycoprotein factor that facilitates hepatic tumor progression. Cancer Res 1998, 58(13):2881-2887.
81. Yang X., Bhaumik M., Bhattacharyya R., Gong S., Rogler C.E., Stanley P. New evidence for an extra-hepatic role of N- acetylglucosaminyltransferase III in the progression of diethylnitrosamine-induced liver tumors in mice. Cancer Res 2000, 60(12):3313-3319.
82. Taniguchi N., Miyoshi E., Gu J., Honke K., Matsumoto A. Decoding sugar functions by identifying target glycoproteins. Curr Opin Struct Biol 2006, 16(5):561-566.
83. Yoshimura M., Ihara Y., Matsuzawa Y., Taniguchi N. Aberrant glycosylation of E-cadherin enhances cell-cell binding to suppress metastasis. J Biol Chem 1996, 271(23):13811-13815.
84. Akasaka-Manya K., Manya H., Sakurai Y., Wojczyk B.S., Kozutsumi Y., Saito Y., et al. Protective effect of N-glycan bisecting GlcNAc residues on {beta}-amyloid production in Alzheimer's disease. Glycobiology 2010, 20(1):99-106.
85. Gleeson P.A., Schachter H. Control of glycoprotein synthesis. VIII. UDP-GlcNAc:GnGn (GlcNAc to Manα1-3) β4-N-acetylglucosaminyltransferase IV, an enzyme in hen oviduct which adds GlcNAc in β1-4 linkage to the α1-3-linked Man residue of the trimannosyl core of N-glycosyl oligosaccharides to form a triantennary structure. J Biol Chem 1983, 258(10):6162-6173.
86. Schachter H., Narasimhan S., Gleeson P., Vella G. Control of branching during the biosynthesis of asparagine-linked oligosaccharides. Can J Biochem Cell Biol 1983, 61(9):1049-1066.
87. Oguri S., Yoshida A., Minowa M.T., Takeuchi M. Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes. Glycoconj J 2006, 23(7-8):473-480.
88. Ohtsubo K., Takamatsu S., Minowa M.T., Yoshida A., Takeuchi M., Marth J.D. Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes. Cell 2005, 123(7):1307-1321.
89. Takamatsu S., Oguri S., Minowa M.T., Yoshida A., Nakamura K., Takeuchi M., et al. Unusually high expression of N-acetylglucosaminyltransferase-IVa in human choriocarcinoma cell lines: a possible enzymatic basis of the formation of abnormal biantennary sugar chain. Cancer Res 1999, 59(16):3949-3953.
90. Ide Y., Miyoshi E., Nakagawa T., Gu J., Tanemura M., Nishida T., et al. Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb (GnT-IVa and b) in pancreatic cancer. Biochem Biophys Res Commun 2006, 341(2):478-482.
91. Dennis J.W. N-Acetylglucosaminyltransferase-V. Handbook of glycosyltransferases and related genes 2002, 94-101. Springer-Verlag, Tokyo, Japan. N. Taniguchi, K. Honke, M. Fukuda (Eds.).
92. Alverez K., Haswell C., St Clair M., Perng G.S., Shorebah M., Pierce M., et al. Sequences of the mouse N-acetylglucosaminyltransferase V (Mgat5) mRNA and an mRNA expressed by an Mgat5-deficient cell line. Glycobiology 2002, 12(7):389-394.
93. Granovsky M., Fata J., Pawling J., Muller W.J., Khokha R., Dennis J.W. Suppression of tumor growth and metastasis in Mgat5-deficient mice. Nat Med 2000, 6(3):306-312.
94. Longmore G.D., Schachter H. Control of glycoprotein synthesis. VI. Product identification and substrate specificity studies of the GDP-l-Fucose:2-acetamido-2-deoxy-β-d-glucoside (Fuc to Asn-linked GlcNAc) 6-α-l-fucosyltransferase in a Golgi-rich fraction form porcine liver. Carbohydr Res 1982, 100:365-392.
95. Wang X., Inoue S., Gu J., Miyoshi E., Noda K., Li W., et al. Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice. Proc Natl Acad Sci USA 2005, 102(44):15791-15796.
96. Schachter H., Chen S., Zhang W., Spence A.M., Zhu S., Callahan J.W., et al. Functional post-translational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans. Biochem Soc Symp 2002, 69:1-21.