Semi-quantitative RT-PCR-based assay, improved by Southern hybridization technique, for polarity-specific influenza virus RNAs in cultured cells

Journal of Virological Methods

Volumn 106, Issue 1, 2002, Pages 125-134

  Uchide, Noboru ^a   Ohyama, Kunio ^a   Bessho, Toshio ^b   Yamakawa, Toshio ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b Yoneyama Maternity Hospital   (Japan)

Author keywords

Hemagglutinin; Influenza virus; Quantitative analysis; RT PCR; Southern hybridization

Indexed keywords

COMPLEMENTARY DNA; INFLUENZA VIRUS HEMAGGLUTININ; MESSENGER RNA; VIRUS RNA;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; DNA PROBE; EMBRYO; FETUS; GENE AMPLIFICATION; HUMAN; HUMAN CELL; INFLUENZA; INFLUENZA VIRUS A; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; VIRUS DETECTION; VIRUS TITRATION;

ANIMALS; ANTIVIRAL AGENTS; BLOTTING, SOUTHERN; CELL POLARITY; CELLS, CULTURED; CHICK EMBRYO; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HUMANS; INFLUENZA A VIRUS; MICROBIAL SENSITIVITY TESTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBAVIRIN; RNA, VIRAL;

INFLUENZA VIRUS;

EID: 0036803364     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00143-X     Document Type: Article

References (19)

1. Beaton A.R., Krug R.M. Synthesis of the templates for influenza virion RNA replication in vitro. Proc. Natl. Acad. Sci. USA. 81:1984;4682-4686.
2. Boener B., Weigelt W., Buhk H.-J., Castrucci G., Ludwig H. A sensitive and specific PCR/Southern blot assay for detection of bovine herpesvirus 4 in calves infected experimentally. J. Virol. Methods. 83:1999;169-180.
3. Eriksson B., Helgstrand E., Johansson N.G., Larsson A., Misiorny A., Noren J.O., Philipson L., Stenberg K., Stening G., Stridh S., Öberg B. Inhibition of influenza virus ribonucleic acid polymerase by ribavirin triphosphate. Antimicrob. Agents Chemother. 11:1977;946-951.
4. Foley K.P., Leonard M.W., Engel J.D. Quantitation of RNA using the polymerase chain reaction. Trends Genet. 9:1993;380-385.
5. Lo Y.-M.D., Mehal W.Z., Fleming K.A. Rapid production of vector-free biotinylated probes using the polymerase chain reaction. Nucl. Acids Res. 16:1988;8719.
6. Majde J.A., Guha-Thakurta N., Chen Z., Bredow S., Krueger J.M. Spontaneous release of stable double-stranded RNA into the extracellular medium by influenza virus-infected MDCK epithelial cells: implications for the viral acute phase response. Arch. Virol. 143:1998;2371-2380.
7. Ohyama K., Oka K., Emura A., Tamura H., Suga T., Bessho T., Hirakawa S., Yamakawa T. Suppression of apoptotic cell death progressed in vitro with incubation of the chorion laeve tissues of human fetal membranes by glucocorticoid. Biol. Pharm. Bull. 21:1998;1024-1029.
8. Rimmelzwaan G.F., Baars M., Claas E.C.J., Osterhaus A.D.M.E. Comparison of RNA hybridization, hemagglutination assay, titration of infectious virus and immunofluorescence as methods for monitoring influenza virus replication in vitro. J. Virol. Methods. 74:1998;57-66.
9. Salomon N., Underwood R., Doyle M.V., Wang A., Libby P. Increased apolipoprotein E and c-fms gene expression without elevated interleukin 1 or 6 mRNA levels indicates selective activation of macrophage functions in advanced human atheroma. Proc. Natl. Acad. Sci. USA. 89:1992;2814-2818.
10. Shambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. 1989;14.1-14.35 Cold Spring Harbor Laboratory, New York.
11. Shapiro G.I., Krug R.M. Influenza virus RNA replication in vitro: synthesis of viral template RNAs and virion RNAs in the absence of an added primer. J. Virol. 62:1988;2285-2290.
12. Singer V.L., Jones L.J., Yue S.T., Haugland R.P. Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation. Anal. Biochem. 249:1997;228-238.
13. Stray S.J., Air G.M. Apoptosis by influenza viruses correlates with efficiency of viral mRNA synthesis. Virus Res. 77:2001;3-17.
14. Takeuchi K., Nagata K., Ishihama A. In vitro synthesis of influenza viral RNA: characterization of an isolated nuclear system that supports transcription of influenza viral RNA. J. Biochem. 101:1987;837-845.
15. Uchide N., Ohyama K., Yuan B., Bessho T., Yamakawa T. Apoptosis in cultured human fetal membrane cells infected with influenza virus. Biol. Pharm. Bull. 25:2002;109-114.
16. Urabe M., Tanaka T., Odagiri T., Tashiro M., Tobita K. Persistence of viral genes in a variant of MDBK cell after productive replication of a mature of influenza virus A/WSN. Arch. Virol. 128:1993;97-110.
17. Winter G., Fields S., Brownlee G.G. Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype. Nature. 292:1981;72-75.
18. Wray S.K., Gilbert B.E., Noall M.W., Knight V. Mode of action of ribavirin: effect of nucleotide pool alterations on influenza virus ribonucleoprotein synthesis. Antiviral Res. 5:1985;29-37.
19. Yamada A., Imanishi J., Nakajima E., Nakajima K., Nakajima S. Detection of influenza viruses in throat swab by using polymerase chain reaction. Microbiol. Immunol. 35:1991;259-265.