Development of a multiplex real-time PCR assay using SYBR Green 1 chemistry for simultaneous detection and subtyping of H9N2 influenza virus type A

Journal of Virological Methods

Volumn 144, Issue 1-2, 2007, Pages 57-64

  Ong, Wee Theng ^a   Omar, Abdul Rahman ^a   Ideris, Aini ^a   Hassan, Sharifah Syed ^b  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b VETERINARY RESEARCH INSTITUTE   (Malaysia)

Author keywords

Avian influenza virus; Melting temperature (Tm); Multiplex; Rapid detection and subtyping; Sensitive; Specific; SYBR Green 1 based real time reverse transcriptase polymerase chain reaction (RRT PCR)

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CHICKEN; CONTROLLED STUDY; GERMFREE ANIMAL; INFLUENZA; INFLUENZA VIRUS A H9N2; MELTING POINT; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS INFECTION; VIRUS ISOLATION;

ANIMALS; CHICKENS; INFLUENZA A VIRUS, H9N2 SUBTYPE; INFLUENZA IN BIRDS; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

AVES; AVIAN INFLUENZA VIRUS; GALLUS GALLUS; INFLUENZA A VIRUS;

EID: 34547112040     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.03.019     Document Type: Article

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