Global assessment of protein turnover in recombinant antibody producing myeloma cells

Journal of Biotechnology

Volumn 148, Issue 4, 2010, Pages 182-193

  Yee, Joon Chong ^a   Jacob, Nitya M ^a   Jayapal, Karthik P ^a   Kok, Yee Jiun ^c   Philp, Robin ^c   Griffin, Timothy J ^b   Hu, Wei Shou ^a  

^a University of Minnesota   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

^c AGENCY FOR SCIENCE   (Singapore)

Author keywords

NS0; Protein turnover; Proteomics; Recombinant IgG; SILAC

Indexed keywords

CELLULAR PROTEINS; DECAY CURVES; DEGRADATION RATE CONSTANTS; HEAVY AND LIGHT CHAINS; HIGH CONFIDENCE; IDENTIFICATION OF PROTEINS; IMMUNOGLOBULIN G; KINETIC BEHAVIOR; MYELOMA CELL; MYELOMA CELL LINE; PROTEOMIC ANALYSIS; PROTEOMICS; RECOMBINANT ANTIBODY; SPECIFIC GROWTH RATE; TIME POINTS; TURNOVER RATE;

AMINO ACIDS; CELL CULTURE; DEGRADATION; GROWTH KINETICS; MOLECULAR BIOLOGY; RADIOACTIVITY; RATE CONSTANTS;

PROTEINS;

ARGININE; IMMUNOGLOBULIN G; RECOMBINANT ANTIBODY;

ARTICLE; CELL GROWTH; CELL TRANSFER; CONTROLLED STUDY; DNA TRANSCRIPTION; GROWTH RATE; HUMAN; HUMAN CELL; KINETICS; MYELOMA CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN METABOLISM; PROTEIN SECRETION; PROTEOMICS; RNA PROCESSING; RNA TRANSLATION;

ANTIBODIES, MONOCLONAL; CELL LINE; HUMANS; IMMUNOGLOBULIN G; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLIC CLEARANCE RATE; MULTIPLE MYELOMA; MYELOMA PROTEINS; PEPTIDE MAPPING; PROTEOME; RECOMBINANT PROTEINS;

EID: 77955049920     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.06.005     Document Type: Article

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