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Volumn 45, Issue 2, 2010, Pages 129-139

Recombinant baculovirus as a highly potent vector for gene therapy of human colorectal carcinoma: Molecular cloning, expression, and in vitro characterization

Author keywords

BacMam; Baculovirus; Biosafety; Colorectal cancer; Gene therapy; Therapeutic deliverys; Transduction

Indexed keywords

ANIMAL VIRUS; AUTOGRAPHA CALIFORNICA; BACULOVIRUS; CANCER GENE THERAPY; CLINICAL TRIAL; COLORECTAL CANCER; COLORECTAL CANCER CELL; COLORECTAL CARCINOMA; COMBINED EFFECT; FACTORIAL DESIGN; GENE DELIVERY; GENE DELIVERY SYSTEMS; IN-VITRO; INCUBATION TIME; INSECT CELLS; MOLECULAR CLONING; MULTIPLICITY OF INFECTIONS; RECOMBINANT BACULOVIRUS; SAFETY CONCERNS; THERAPEUTIC STRATEGY; TRANSDUCTION EFFICIENCY; VIROTHERAPY;

EID: 77954951816     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9248-7     Document Type: Article
Times cited : (17)

References (52)
  • 2
    • 0033616484 scopus 로고    scopus 로고
    • Colorectal cancer
    • Midgley, R., & Kerr, D. (1999). Colorectal cancer. Lancet, 353, 391-399.
    • (1999) Lancet , vol.353 , pp. 391-399
    • Midgley, R.1    Kerr, D.2
  • 5
    • 77954959852 scopus 로고    scopus 로고
    • Gene therapy for colon cancer using survivin antisense expressing replication-incompetent adenoviral vectors
    • Yamamoto, T. Y., Okuda, J. O., Toyoda, M. T., & Tanigawa, N. T. (2002). Gene therapy for colon cancer using survivin antisense expressing replication-incompetent adenoviral vectors. International Journal of Cancer, (Suppl 13), 125.
    • (2002) International Journal of Cancer , Issue.SUPPL. 13 , pp. 125
    • Yamamoto, T.Y.1    Okuda, J.O.2    Toyoda, M.T.3    Tanigawa, N.T.4
  • 6
    • 0036162121 scopus 로고    scopus 로고
    • From molecular changes to customised therapy
    • Hemminki, A. (2002). From molecular changes to customised therapy. European Journal of Cancer, 38, 333-338.
    • (2002) European Journal of Cancer , vol.38 , pp. 333-338
    • Hemminki, A.1
  • 7
    • 2442635383 scopus 로고    scopus 로고
    • Modified adenoviruses for cancer gene therapy
    • DOI 10.1002/ijc.20129
    • Kanerva, A., & Hemminki, A. (2004). Modified adenoviruses for cancer gene therapy. International Journal of Cancer, 110, 475-480. (Pubitemid 38658011)
    • (2004) International Journal of Cancer , vol.110 , Issue.4 , pp. 475-480
    • Kanerva, A.1    Hemminki, A.2
  • 8
    • 0035158045 scopus 로고    scopus 로고
    • In vivo gene therapy for colon cancer using adenovirus-mediated, transfer of the fusion gene cytosine deaminase and uracil phosphoribosyltransferase
    • Chung-Faye, G. A., Chen, M. J., Green, N. K., Burton, A., Anderson, D., Mautner, V., et al. (2001). In vivo gene therapy for colon cancer using adenovirus-mediated, transfer of the fusion gene cytosine deaminase and uracil phosphoribosyltransferase. Gene Therapy, 8, 1547-1554.
    • (2001) Gene Therapy , vol.8 , pp. 1547-1554
    • Chung-Faye, G.A.1    Chen, M.J.2    Green, N.K.3    Burton, A.4    Anderson, D.5    Mautner, V.6
  • 10
    • 65649142589 scopus 로고    scopus 로고
    • A novel A33 promoterbased conditionally replicative adenovirus suppresses tumor growth and eradicates hepatic metastases in human colon cancer models
    • Cafferata, E. G., Maccio, D. R., Lopez, M. V., Viale, D. L., Carbone, C., Mazzolini, G., et al. (2009). A novel A33 promoterbased conditionally replicative adenovirus suppresses tumor growth and eradicates hepatic metastases in human colon cancer models. Clinical Cancer Research, 15, 3037-3049.
    • (2009) Clinical Cancer Research , vol.15 , pp. 3037-3049
    • Cafferata, E.G.1    Maccio, D.R.2    Lopez, M.V.3    Viale, D.L.4    Carbone, C.5    Mazzolini, G.6
  • 11
    • 67650409973 scopus 로고    scopus 로고
    • Oncolytic adenovirus delivering herpes simplex virus thymidine kinase suicide gene reduces the growth of human retinoblastoma in an in vivo mouse model
    • Huang, Q., Ji, X., Zhang, J., Cheng, L., Wei, F., Li, H., et al. (2009). Oncolytic adenovirus delivering herpes simplex virus thymidine kinase suicide gene reduces the growth of human retinoblastoma in an in vivo mouse model. Experimental Eye Research, 89, 193-199.
    • (2009) Experimental Eye Research , vol.89 , pp. 193-199
    • Huang, Q.1    Ji, X.2    Zhang, J.3    Cheng, L.4    Wei, F.5    Li, H.6
  • 13
    • 65949114070 scopus 로고    scopus 로고
    • E1B 55-kDa deleted, Ad5/F35 fiber chimeric adenovirus, a potential oncolytic agent for B-lymphocytic malignancies
    • Wang, G., Li, G., Liu, H., Yang, C., Yang, X., Jin, J., et al. (2009). E1B 55-kDa deleted, Ad5/F35 fiber chimeric adenovirus, a potential oncolytic agent for B-lymphocytic malignancies. Journal of Gene Medicine, 11, 477-485.
    • (2009) Journal of Gene Medicine , vol.11 , pp. 477-485
    • Wang, G.1    Li, G.2    Liu, H.3    Yang, C.4    Yang, X.5    Jin, J.6
  • 14
    • 67651083253 scopus 로고    scopus 로고
    • INGN 007, an oncolytic adenovirus vector, replicates in Syrian hamsters but not mice: Comparison of biodistribution studies
    • Ying, B., Toth, K., Spencer, J. F., Meyer, J., Tollefson, A. E., Patra, D., et al. (2009). INGN 007, an oncolytic adenovirus vector, replicates in Syrian hamsters but not mice: comparison of biodistribution studies. Cancer Gene Therapy, 16, 625-637.
    • (2009) Cancer Gene Therapy , vol.16 , pp. 625-637
    • Ying, B.1    Toth, K.2    Spencer, J.F.3    Meyer, J.4    Tollefson, A.E.5    Patra, D.6
  • 15
    • 0033914616 scopus 로고    scopus 로고
    • Combined suicide gene therapy for human colon cancer cells using adenovirus-mediated transfer of Escherichia coli cytosine deaminase gene and Escherichia coli uracil phosphoribosyltransferase gene with 5-fluorocytosine
    • Koyama, F., Sawada, H., Hirao, T., Fujii, H., Hamada, H., & Nakano, H. (2000). Combined suicide gene therapy for human colon cancer cells using adenovirus-mediated transfer of Escherichia coli cytosine deaminase gene and Escherichia coli uracil phosphoribosyltransferase gene with 5-fluorocytosine. Cancer Gene Therapy, 7, 1015-1022. (Pubitemid 30483481)
    • (2000) Cancer Gene Therapy , vol.7 , Issue.7 , pp. 1015-1022
    • Koyama, F.1    Sawada, H.2    Hirao, T.3    Fujii, H.4    Hamada, H.5    Nakano, H.6
  • 18
    • 0026755552 scopus 로고
    • Production of a functional monoclonal-antibody recognizing human colorectal-carcinoma cells from a baculovirus expression system
    • Nesbit, M., Fu, Z. F., Mcdonaldsmith, J., Steplewski, Z., & Curtis, P. J. (1992). Production of a functional monoclonal-antibody recognizing human colorectal-carcinoma cells from a baculovirus expression system. Journal of Immunological Methods, 151, 201-208.
    • (1992) Journal of Immunological Methods , vol.151 , pp. 201-208
    • Nesbit, M.1    Fu, Z.F.2    Mcdonaldsmith, J.3    Steplewski, Z.4    Curtis, P.J.5
  • 19
    • 0042629626 scopus 로고    scopus 로고
    • Regression of primary murine colon cancer and occult liver metastasis by intralesional injection of lyophilized preparation of insect cells producing murine interferon-beta
    • Ozawa, S., Lu, W. X., Bucana, C. D., Kanayama, H. O., Shinohara, H., Fidler, I. J., et al. (2003). Regression of primary murine colon cancer and occult liver metastasis by intralesional injection of lyophilized preparation of insect cells producing murine interferon-beta. International Journal of Oncology, 22, 977-984.
    • (2003) International Journal of Oncology , vol.22 , pp. 977-984
    • Ozawa, S.1    Lu, W.X.2    Bucana, C.D.3    Kanayama, H.O.4    Shinohara, H.5    Fidler, I.J.6
  • 20
    • 0035977949 scopus 로고    scopus 로고
    • Combination treatment for osteosarcoma with baculoviral vector mediated gene therapy (p53) and chemotherapy (adriamycin)
    • Song, S. U., & Boyce, F. M. (2001). Combination treatment for osteosarcoma with baculoviral vector mediated gene therapy (p53) and chemotherapy (adriamycin). Experimental and Molecular Medicine, 33, 46-53.
    • (2001) Experimental and Molecular Medicine , vol.33 , pp. 46-53
    • Song, S.U.1    Boyce, F.M.2
  • 21
    • 63449115326 scopus 로고    scopus 로고
    • Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene
    • Huang, W., Tian, X. L., Wu, Y. L., Zhong, J., Yu, L. F., Hu, S. P., et al. (2008). Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene. World Journal of Gastroenterology, 14, 5810-5815.
    • (2008) World Journal of Gastroenterology , vol.14 , pp. 5810-5815
    • Huang, W.1    Tian, X.L.2    Wu, Y.L.3    Zhong, J.4    Yu, L.F.5    Hu, S.P.6
  • 22
    • 58149199027 scopus 로고    scopus 로고
    • Nonviral production of human interleukin-7 in spodoptera frugiperda insect cells as a soluble recombinant protein
    • doi:10.1155/2009/637942
    • Mirzaei, M., Xu, Y., Elias, C. B., & Prakash, S. (2009). Nonviral production of human interleukin-7 in spodoptera frugiperda insect cells as a soluble recombinant protein. Journal of Biomedicine and Biotechnology, 2009, 8 pp. doi:10.1155/2009/ 637942.
    • (2009) Journal of Biomedicine and Biotechnology , vol.2009
    • Mirzaei, M.1    Xu, Y.2    Elias, C.B.3    Prakash, S.4
  • 23
    • 34249807610 scopus 로고    scopus 로고
    • High cell density fed batch and perfusion processes for stable non-viral expression of secreted alkaline phosphatase (SEAP) using insect cells: Comparison to a batch Sf-9-BEV system
    • Jardin, B. A., Montes, J., Lanthier, S., Tran, R., & Elias, C. (2007). High cell density fed batch and perfusion processes for stable non-viral expression of secreted alkaline phosphatase (SEAP) using insect cells: comparison to a batch Sf-9-BEV system. Biotechnology and Bioengineering, 97, 332-345.
    • (2007) Biotechnology and Bioengineering , vol.97 , pp. 332-345
    • Jardin, B.A.1    Montes, J.2    Lanthier, S.3    Tran, R.4    Elias, C.5
  • 24
    • 46149116542 scopus 로고    scopus 로고
    • Expression of SEAP (secreted alkaline phosphatase) by baculovirus mediated transduction of HEK 293 cells in a hollow fiber bioreactor system
    • Jardin, B. A., Zhao, Y., Selvaraj, M., Montes, J., Tran, R., Prakash, S., et al. (2008). Expression of SEAP (secreted alkaline phosphatase) by baculovirus mediated transduction of HEK 293 cells in a hollow fiber bioreactor system. Journal of Biotechnology, 135, 272-280.
    • (2008) Journal of Biotechnology , vol.135 , pp. 272-280
    • Jardin, B.A.1    Zhao, Y.2    Selvaraj, M.3    Montes, J.4    Tran, R.5    Prakash, S.6
  • 25
    • 0034813062 scopus 로고    scopus 로고
    • Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes
    • Bilello, J. P., Delaney, W. E., Boyce, F. M., & Isom, H. C. (2001). Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes. Journal of Virology, 75, 9857-9871.
    • (2001) Journal of Virology , vol.75 , pp. 9857-9871
    • Bilello, J.P.1    Delaney, W.E.2    Boyce, F.M.3    Isom, H.C.4
  • 27
    • 26644449182 scopus 로고    scopus 로고
    • Adeno-associated virus inverted terminal repeats improve neuronal transgene expression mediated by baculoviral vectors in rat brain\
    • Wang, C. Y., & Wang, S. (2005). Adeno-associated virus inverted terminal repeats improve neuronal transgene expression mediated by baculoviral vectors in rat brain. Human Gene Therapy, 16, 1219-1226.
    • (2005) Human Gene Therapy , vol.16 , pp. 1219-1226
    • Wang, C.Y.1    Wang, S.2
  • 29
    • 33646806832 scopus 로고    scopus 로고
    • Current developments in adenovirus-based cancer gene therapy
    • Rein, D. T., Breidenbach, M., & Curiel, D. T. (2006). Current developments in adenovirus-based cancer gene therapy. Future Oncology, 2, 137-143.
    • (2006) Future Oncology , vol.2 , pp. 137-143
    • Rein, D.T.1    Breidenbach, M.2    Curiel, D.T.3
  • 30
    • 33745227413 scopus 로고    scopus 로고
    • Enhanced baculovirus-mediated transduction of human cancer cells by tumor-homing peptides
    • Makela, A. R., Matilainen, H., White, D. J., Ruoslahti, E., & Oker-Blom, C. (2006). Enhanced baculovirus-mediated transduction of human cancer cells by tumor-homing peptides. Journal of Virology, 80, 6603-6611.
    • (2006) Journal of Virology , vol.80 , pp. 6603-6611
    • Makela, A.R.1    Matilainen, H.2    White, D.J.3    Ruoslahti, E.4    Oker-Blom, C.5
  • 31
    • 0037177002 scopus 로고    scopus 로고
    • Efficient and tightly regulated expression systems for the human cytomegalovirus major transactivator protein IE2p86 in permissive cells
    • Kronschnabl, M., Marschall, M., & Stamminger, T. (2002). Efficient and tightly regulated expression systems for the human cytomegalovirus major transactivator protein IE2p86 in permissive cells. Virus Research, 83, 89-102.
    • (2002) Virus Research , vol.83 , pp. 89-102
    • Kronschnabl, M.1    Marschall, M.2    Stamminger, T.3
  • 32
    • 0033664131 scopus 로고    scopus 로고
    • Baculovirus-mediated gene transfer into pancreatic islet cells
    • Ma, L., Tamarina, N., Wang, Y., Kuznetsov, A., Patel, N., Kending, C., et al. (2000). Baculovirus-mediated gene transfer into pancreatic islet cells. Diabetes, 49, 1986-1991.
    • (2000) Diabetes , vol.49 , pp. 1986-1991
    • Ma, L.1    Tamarina, N.2    Wang, Y.3    Kuznetsov, A.4    Patel, N.5    Kending, C.6
  • 33
    • 34147097547 scopus 로고    scopus 로고
    • Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells
    • Zeng, J., Du, J., Zhao, Y., Palanisamy, N., & Wang, S. (2007). Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells. Stem Cells, 25, 1055-1061.
    • (2007) Stem Cells , vol.25 , pp. 1055-1061
    • Zeng, J.1    Du, J.2    Zhao, Y.3    Palanisamy, N.4    Wang, S.5
  • 34
    • 67349276428 scopus 로고    scopus 로고
    • Baculovirus transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation
    • Chuang, C. K., Wong, T. H., Hwang, S. M., Chang, Y. H., Chen, G. Y., Chiu, Y. C., et al. (2009). Baculovirus transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. Molecular Therapy, 17, 889-896.
    • (2009) Molecular Therapy , vol.17 , pp. 889-896
    • Chuang, C.K.1    Wong, T.H.2    Hwang, S.M.3    Chang, Y.H.4    Chen, G.Y.5    Chiu, Y.C.6
  • 35
    • 31144454339 scopus 로고    scopus 로고
    • Comparison between recombinant baculo- and adenoviral-vectors as transfer system in cardiovascular cells
    • Grassi, G., Kohn, H., Dapas, B., Farra, R., Platz, J., Engel, S., et al. (2006). Comparison between recombinant baculo- and adenoviral-vectors as transfer system in cardiovascular cells. Archives of Virology, 151, 255-271.
    • (2006) Archives of Virology , vol.151 , pp. 255-271
    • Grassi, G.1    Kohn, H.2    Dapas, B.3    Farra, R.4    Platz, J.5    Engel, S.6
  • 36
    • 0036979916 scopus 로고    scopus 로고
    • Induction of apoptosis in rabbit kidney cell under high-level expression of bovine herpesvirus 1 U(s) ORF8 product
    • Nakamichi, K., Matsumoto, Y., Tohya, Y., & Otsuka, H. (2002). Induction of apoptosis in rabbit kidney cell under high-level expression of bovine herpesvirus 1 U(s) ORF8 product. Intervirology, 45, 85-93.
    • (2002) Intervirology , vol.45 , pp. 85-93
    • Nakamichi, K.1    Matsumoto, Y.2    Tohya, Y.3    Otsuka, H.4
  • 37
    • 2942744801 scopus 로고    scopus 로고
    • A rapid and efficient method to express target genes in mammalian cells by baculovirus
    • Cheng, T., Xu, C. Y., Wang, Y. B., Chen, M., Wu, T., Zhang, J., et al. (2004). A rapid and efficient method to express target genes in mammalian cells by baculovirus. World Journal of Gastroenterology, 10, 1612-1618.
    • (2004) World Journal of Gastroenterology , vol.10 , pp. 1612-1618
    • Cheng, T.1    Xu, C.Y.2    Wang, Y.B.3    Chen, M.4    Wu, T.5    Zhang, J.6
  • 38
    • 33644962970 scopus 로고    scopus 로고
    • Baculovirus-mediated gene expression in chicken primary cells
    • Ping, W., Ge, J., Li, S., Zhou, H., Wang, K., Feng, Y., et al. (2006). Baculovirus-mediated gene expression in chicken primary cells. Avian Diseases, 50, 59-63.
    • (2006) Avian Diseases , vol.50 , pp. 59-63
    • Ping, W.1    Ge, J.2    Li, S.3    Zhou, H.4    Wang, K.5    Feng, Y.6
  • 40
    • 0034023958 scopus 로고    scopus 로고
    • Gene therapy: A rocky start to the new millennium
    • Senior, K. (2000). Gene therapy: a rocky start to the new millennium. Molecular Medicine Today, 6, 93.
    • (2000) Molecular Medicine Today , vol.6 , pp. 93
    • Senior, K.1
  • 41
    • 0035005986 scopus 로고    scopus 로고
    • Incorporation of decay-accelerating factor into the baculovirus envelope generates complement-resistant gene transfer vectors
    • Huser, A., Rudolph, M., & Hofmann, C. (2001). Incorporation of decay-accelerating factor into the baculovirus envelope generates complement-resistant gene transfer vectors. Nature Biotechnology, 19, 451-455.
    • (2001) Nature Biotechnology , vol.19 , pp. 451-455
    • Huser, A.1    Rudolph, M.2    Hofmann, C.3
  • 42
    • 0031942299 scopus 로고    scopus 로고
    • Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system
    • Hofmann, C., & Strauss, M. (1998). Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system. Gene Therapy, 5, 531-536.
    • (1998) Gene Therapy , vol.5 , pp. 531-536
    • Hofmann, C.1    Strauss, M.2
  • 44
    • 35948971850 scopus 로고    scopus 로고
    • Baculovirus-mediated expression of p35 confers resistance to apoptosis in human embryo kidney 293 cells
    • Song, J., Liang, C.-y., & Chen, X.-w. (2007). Baculovirus-mediated expression of p35 confers resistance to apoptosis in human embryo kidney 293 cells. Virologica Sinica, 22, 389-396.
    • (2007) Virologica Sinica , vol.22 , pp. 389-396
    • Song, J.1    Liang, C.-y.2    Chen, X.-w.3
  • 46
    • 0041383924 scopus 로고    scopus 로고
    • Clinical development of gene therapy for colorectal cancer
    • Kerr, D. (2003). Clinical development of gene therapy for colorectal cancer. Nature Reviews Cancer, 3, 615-622.
    • (2003) Nature Reviews Cancer , vol.3 , pp. 615-622
    • Kerr, D.1
  • 47
    • 0034469507 scopus 로고    scopus 로고
    • Gene therapy for carcinoma of the breast: Therapeutic genetic correction strategies
    • Obermiller, P. S., Tait, D. L., & Holt, J. T. (2000). Gene therapy for carcinoma of the breast: therapeutic genetic correction strategies. Breast Cancer Research, 2, 28-31.
    • (2000) Breast Cancer Research , vol.2 , pp. 28-31
    • Obermiller, P.S.1    Tait, D.L.2    Holt, J.T.3
  • 48
    • 0035671396 scopus 로고    scopus 로고
    • SMAD4/DPC4 and pancreatic cancer survival- Commentary re: M. Tascilar et al., The SMAD4 protein and prognosis of pancreatic ductal adenocarcinoma
    • 2001
    • Liu, F. (2001). SMAD4/DPC4 and pancreatic cancer survival- Commentary re: M. Tascilar et al., The SMAD4 protein and prognosis of pancreatic ductal adenocarcinoma. Clinical Cancer Research, 7: 4115-4121, 2001.
    • (2001) Clinical Cancer Research , vol.7 , pp. 4115-4121
    • Liu, F.1
  • 50
    • 0033199168 scopus 로고    scopus 로고
    • p53 selective and nonselective replication of an E1B-deleted adenovirus in hepatocellular carcinoma
    • Vollmer, C. M., Ribas, A., Butterfield, L. H., Dissette, V. B., Andrews, K. J., Eilber, F. C., et al. (1999). p53 selective and nonselective replication of an E1B-deleted adenovirus in hepatocellular carcinoma. Cancer Research, 59, 4369-4374.
    • (1999) Cancer Research , vol.59 , pp. 4369-4374
    • Vollmer, C.M.1    Ribas, A.2    Butterfield, L.H.3    Dissette, V.B.4    Andrews, K.J.5    Eilber, F.C.6
  • 51
    • 23944494575 scopus 로고    scopus 로고
    • Fractalkine gene therapy for neuroblastoma is more effective in combination with targeted IL-2
    • Zeng, Y., Jiang, J., Huebener, N., Wenkel, J., Gaedicke, G., Xiang, R., et al. (2005). Fractalkine gene therapy for neuroblastoma is more effective in combination with targeted IL-2. Cancer Letters, 228, 187-193.
    • (2005) Cancer Letters , vol.228 , pp. 187-193
    • Zeng, Y.1    Jiang, J.2    Huebener, N.3    Wenkel, J.4    Gaedicke, G.5    Xiang, R.6
  • 52
    • 34848866647 scopus 로고    scopus 로고
    • Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4
    • Todaro, M., Alea, M. P., Di Stefano, A. B., Cammareri, P., Vermeulen, L., Lovino, F., et al. (2007). Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4. Cell Stem Cell, 1, 389-402.
    • (2007) Cell Stem Cell , vol.1 , pp. 389-402
    • Todaro, M.1    Alea, M.P.2    Di Stefano, A.B.3    Cammareri, P.4    Vermeulen, L.5    Lovino, F.6


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