Expression of SEAP (secreted alkaline phosphatase) by baculovirus mediated transduction of HEK 293 cells in a hollow fiber bioreactor system

Journal of Biotechnology

Volumn 135, Issue 3, 2008, Pages 272-280

  Jardin, B A ^a   Zhao, Y ^b   Selvaraj, M ^c   Montes, J ^a   Tran, R ^a   Prakash, S ^d   Elias, C B ^e  

^a Biotchnology Research Institute   (Canada)

^b QSV Biologics Ltd   (Canada)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d MCGILL UNIVERSITY   (Canada)

^e Sanofi Pasteur   (Canada)

Author keywords

BacMam baculovirus; CHO K1 cells; CMV promoter; Factorial design; HEK 293A cells; Hollow fiber (HBFR); Sf9; TFF; Wave bioreactor

Indexed keywords

ANIMAL CELL CULTURE; ARCHITECTURAL DESIGN; BACTERIOPHAGES; BATCH REACTORS; BIOCONVERSION; BIOREACTORS; CELL CULTURE; CELLS; COMPUTER NETWORKS; CURVE FITTING; FIBER OPTICS; GENE EXPRESSION; INDUSTRIAL CHEMICALS; MAMMALS; PHOTOACOUSTIC EFFECT;

(E ,3E) PROCESS; BACULOVIRUS; BIOREACTOR SYSTEMS; CELL DENSITIES; CELL LINES; CHINESE HAMSTER OVARY (CHO); CHINESE HAMSTER OVARY (CHO) CELLS; CONCENTRATION (COMPOSITION); CULTURE CONDITIONS; ELSEVIER (CO); EXPERIMENTAL CONDITIONS; FACTORIAL DESIGNS; GROWTH OF CELLS; HEK 293 CELLS; HISTONE DEACETYLASE (HDAC) INHIBITORS; HOLLOW FIBER BIOREACTOR; HUMAN CYTOMEGALOVIRUS (HCMV); HUMAN EMBRYONIC KIDNEY (HEK); IN VITRO TESTING; MAMMALIAN CELL LINES; MAMMALIAN CELLS; MULTIPLICITY OF INFECTION (MOI); RECOMBINANT BACULOVIRUS; RECOMBINANT PROTEINS; REPORTER PROTEIN; SECRETED ALKALINE PHOSPHATASE (SEAP); SECRETED PROTEINS; TRANSIENT EXPRESSIONS; TRICHOSTATIN A (TSA);

DESIGN OF EXPERIMENTS;

ALKALINE PHOSPHATASE; HISTONE DEACETYLASE INHIBITOR; RECOMBINANT PROTEIN; TRICHOSTATIN A;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BACULOVIRUS; BIOREACTOR; CELL DENSITY; CELL LINE; CELL STRAIN HEK293; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EMBRYO; FACTORIAL DESIGN; HOLLOW FIBER; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; VIRUS INFECTION;

ALKALINE PHOSPHATASE; ANALYSIS OF VARIANCE; ANIMALS; BACULOVIRIDAE; BIOREACTORS; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; FILTRATION; GENETIC VECTORS; HUMANS; INSECTS; TRANSDUCTION, GENETIC;

CRICETULUS GRISEUS; HUMAN HERPESVIRUS 5; MAMMALIA;

EID: 46149116542     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.04.006     Document Type: Article

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