Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine

Journal of Biological Chemistry

Volumn 285, Issue 30, 2010, Pages 22976-22985

  Rowley, Paul A ^a   Kachroo, Aashiq H ^a   Ma, Chien Hui ^a   Maciaszek, Anna D ^b   Guga, Piotr ^b   Jayaram, Makkuni ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b CENTRE OF MOLECULAR AND MACROMOLECULAR STUDIES   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ARGININE RESIDUE; CATALYTIC ACTIVATION; DIESTERS; DIRECT HYDROLYSIS; DNA SUBSTRATES; HYDROLYTIC ACTIVITIES; HYDROXYL GROUPS; NEGATIVE CHARGE; NUCLEOPHILIC ATTACK; PHOSPHATE GROUP; PHOSPHODIESTER BONDS; POSITIVELY CHARGED; RECOMBINANT DNA; SIDE CHAINS; SITE-SPECIFIC; SITE-SPECIFIC RECOMBINATION; WILD TYPES;

DNA; GENES; GENETIC ENGINEERING; HYDROLYSIS; JOINING; NUCLEOPHILES; PHOSPHATASES; SUBSTRATES;

AMINO ACIDS;

ARGININE; DNA; METHYLPHOSPHONIC ACID; PHOSPHATE; RECOMBINASE; TYROSINE;

ARTICLE; DNA RECOMBINATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HYDROLYSIS; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL;

ARGININE; BASE SEQUENCE; BIOCATALYSIS; CATALYTIC DOMAIN; CONSERVED SEQUENCE; DNA; ENDONUCLEASES; HYDROLYSIS; MODELS, MOLECULAR; MUTATION; ORGANOPHOSPHORUS COMPOUNDS; RECOMBINASES; RECOMBINATION, GENETIC; STATIC ELECTRICITY; SUBSTRATE SPECIFICITY; TYROSINE;

EID: 77954889080     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.112292     Document Type: Article

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