Micropropagation of apple - A review

Biotechnology Advances

Volumn 28, Issue 4, 2010, Pages 462-488

  Dobránszki, Judit ^a   Teixeira da Silva, Jaime A ^b  

^a UNIVERSITY OF DEBRECEN   (Hungary)

^b KAGAWA UNIVERSITY   (Japan)

Author keywords

In vitro; Malus sp.; Production of propagation materials; Rooting; Shoot multiplication

Indexed keywords

BASIC AND APPLIED RESEARCH; IN VITRO; INTERNAL AND EXTERNAL FACTORS; MALUS SP.; MICROPROPAGATED PLANTLETS; MICROPROPAGATION; MICROSHOOTS; NEW APPROACHES; PHYSIOLOGICAL STATE; RAPID MULTIPLICATION; SHOOT MULTIPLICATION;

PLANTS (BOTANY);

ACCLIMATION; DECIDUOUS TREE; DISEASE RESISTANCE; FRUIT; GENOTYPE; ROOTSTOCK; VEGETATIVE PROPAGATION;

AGRICULTURE; APPLE; BIOTECHNOLOGY; CULTURE TECHNIQUE; GROWTH, DEVELOPMENT AND AGING; METHODOLOGY; PLANT; PLANT ROOT; REVIEW;

AGRICULTURE; BIOTECHNOLOGY; CULTURE TECHNIQUES; MALUS; PLANT ROOTS; PLANT SHOOTS;

MALUS SP.; MALUS X DOMESTICA;

EID: 77954844352     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2010.02.008     Document Type: Review

References (252)

1. Abbott A.J. Practice and promise of micropropagation of woody species. Acta Hortic 1978, 79:113-127.
2. Abbott A.J., Whiteley E. Culture of Malus tissues in vitro. I. Multiplication of apple plants from isolated shoot apices. Sci Hortic 1976, 4:183-189.
3. Abdul-Kader A.M., Máthé Á., Lászlóffy K. Aspects of the in vitro technology of apple (Malus sp.). Acta Agron Hung 1991, 40:237-251.
4. Abdul-Kader A.M., Máthé Á., Lászlóffy K. In vitro propagation of apple: comparative response of three cultivars to cytokinin and auxin. Acta Hortic 1992, 300:155-162.
5. Abel S., Theologis A. Early genes and auxin action. Plant Physiol 1996, 111:9-17.
6. Abeyaratne W.M., De Silva U.N., Kumari H.M.P.S. Callus induction, plant regeneration and occurrence of somaclonal variation in somatic tissues of some indica rice varieties. Ann Sri Lanka Dept Agric 2004, 6:1-11.
7. Aldwinckle H., Malnoy M. Plant regeneration and transformation in the Rosaceae. Transgenic plant J 2009, 3 (Special Issue 1):1-39. M. Nageswara-Rao, J.R. Soneji (Eds.).
8. Ali Bacha N., Darkazanli K., Abdul-Kader A.M. Direct organogenesis and plantlet multiplication from leaf explants of in vitro-grown shoots of apple (Malus domestica Borkh.) cv. 'Golden Delicious' and MM111 rootstock. Fruit Veg Cereal Sci Biotech 2009, 3:28-34.
9. Ali Bacha N., Al-Rihani K.H., Abdul-Kader A.M. Development of a method for in vitro propagation of the apple local cultivar "Sukari". Bassel al-Assad J Engin Sci Agric Food Chem Biotech 2009, 26:213-234.
10. Al-Rihani Kh., AbdulKader A.M., Dayoub A., Kalhout A.R. In vitro micropropagation of apple rootstock M27. Res J Aleppo Univ Agric Sci Ser 2005, 53:47-66.
11. Al-Tiawni E., Ali Bacha N., Al-Rihani K., Abdul-Kader A. In vitro micropropagation of apple cv. Golden Delicious using tissue culture technique. Bassel al-Assad J Engin Sci Agric Food Chem Biotech 2009, 26:47-64.
12. Alvarez R., Nissen S.J., Sutter E.G. Relationship between indole-3-acetic acid levels in apple (Malus pumila Mill) rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid. Plant Physiol 1989, 89:439-443.
13. Ara H., Jaiswal U., Jaiswal V.S. Synthetic seed: prospects and limitations. Curr Sci 2000, 78:1438-1444.
14. Arias M., Carbonell J., Agustí M. Endogenous free polyamines and their role in fruit set of low and high parthenocarpic ability citrus cultivars. J Plant Physiol 2005, 162:845-853.
15. Auderset G., Givillet S., Micheli J., O'Rourkr J., Ribaux M., Moncousin C. Histological analysis and the evolution of biochemical markers during the in vitro rooting of Malus domestica Borkh. 'Jork 9'. Adv Hortic Sci 1994, 8:5-10.
16. Babbar S.B., Jain R., Walia N. Guar gum as a gelling agent for plant tissue culture media. In Vitro Cell Dev Biol - Plant 2005, 41:258-261.
17. Bahmani R., Karami O., Gholami M. The effect of carbon source and concentration on in vitro shoot proliferation of MM.106 apple rootstock. Fruit Veg Cereal Sci Biotech 2009, 3:35-37.
18. Bahmani R., Karami O., Gholami M. Influence of carbon sources and their concentrations on rooting and hyperhydricity of apple rootstock MM.106. World Appl Sci J 2009, 6:1513-1517.
19. Baraldi R., Fasolo Fabbri Malavasi F., Predieri S., Castagneto M. Effect of potassium humate on apple cv. 'Golden Delicious' cultured in vitro. Plant Cell Tissue Org Cult 1991, 24:187-191.
20. Biedermann I.E.C. Factors affecting establishment and development of Magnolia hybrids in vitro. Acta Hortic 1987, 212:625-629.
21. Biswas M.K., Dutt M., Roy U.K., Islam R., Hossain M. Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Sci Hortic 2009, 122:409-416.
22. Block R., Lankes C. Massnahmen gegen die Verbräunung bei der in-vitro-Etablierung der Apfelunterlage M9. Gartenbauwiss 1996, 61:11-17.
23. Bolar J.P., Norelli J.L., Aldwinckle H.S. An efficient method for rooting and acclimation of micropropagated apple cultivars. HortScience 1998, 33:1251-1252.
24. Bommineni V.R., Mathews H., Samuel S.B., Kramer M., Wagner D.R. A new method for rapid in vitro propagation of apple and pear. HortScience 2001, 36:1102-1106.
25. Bordallo P.N., Silva D.H., Maria J., Cruz C.D., Fontes E.P. Somaclonal variation on in vitro callus culture potato cultivars. Hortic Bras 2004, 22:300-304.
26. Borges de Paiva Neto V., Campos Otoni W. Carbon sources and their osmotic potential in plant tissue culture: does it matter?. Sci Hortic 2003, 97:193-202.
27. Branzanti B., Gianinazzi-Pearson V., Gianinazzi S. Influence of phosphate fertilization on the growth and nutrient status of micropropagated apple infected with endomycorrhizal fungi during the weaning stage. Agronomie 1992, 12:841-845.
28. Brischia R., Piccioni E., Standardi A. Micropropagation and synthetic seed in M.26 apple rootstock (II): a new protocol for production of encapsulated differentiating propagules. Plant Cell Tissue Org Cult 2002, 68:137-141.
29. Caboni E., Lauri P., Damiano C., D'Angeli S. Somaclonal variation induced by adventitious shoot regeneration in pear and apple. Acta Hortic 2000, 530:195-201.
30. Calamar A., De Klerk G.J. Effect of sucrose on adventitious root regeneration in apple. Plant Cell Tissue Org Cult 2002, 70:207-212.
31. Capuano G., Piccioni E., Standardi A. Effect of different treatments on the conversion of M.26 apple rootstock synthetic seeds obtained from encapsulated apical and axillary micropropagated buds. J Hortic Sci Biotechnol 1988, 73:299-305.
32. Chakrabarty D., Hahn E.J., Yoon Y.J., Paek K.Y. Micropropagation of apple rootstock M.9 EMLA using bioreactor. J Hortic Sci Biotechnol 2003, 78:605-609.
33. Cheng T.-Y. Propagating woody plants through tissue culture. Am Nurseryman 1978, 7-14.
34. Chevreau E., Brisset M.N., Paulin J.P., James D.J. Fire blight resistance and genetic trueness-to-type of four somaclonal variants from the apple cultivar Greensleeves. Euphytica 1998, 104:199-205.
35. Ciccoti A.M., Bisognin C., Battocletti I., Salvadori A., Herdemertens M., Jarausch W. Micropropagation of apple proliferation-resistant apomictic Malus sieboldii genotypes. Agron Res 2008, 6:445-458.
36. Ciccoti A.M., Bisognin C., Battocletti I., Salvadori A., Herdemertens M., Wallbrain M., et al. Micropropagation of Malus sieboldii hybrids resistant to apple proliferation disease. Acta Hortic 2009, 839:35-42.
37. Czynczyk A., Bielicki P., Bartosiewicz B. Evaluation of the effect of P 14 rootstock propagated in vitro and in stoolbeds on the growth and yielding of three apple cultivars. J Fruit Ornam Plant Res 2008, 16:25-30.
38. De Klerk G.J. How to measure somaclonal variation. Acta Bot Neerl 1990, 39:129-144.
39. De Klerk G.-J., Ter Brugge J., Keppel M. Successive phases during rooting of microcuttings of Malus. Acta Hortic 1993, 336:249-256.
40. De Klerk G.J., Keppel M., Ter Brugge J., Meekes J. Timing of the phases in adventitious root formation in apple microcuttings. J Exp Bot 1995, 46:965-972.
41. De Klerk G.-J., Ter Brugge J., Marinova S. Toxicity of ethanol during proliferation and adventitious root formation in apple microcuttings in vitro. Biol Plant 1997, 39:105-112.
42. De Klerk G.J., van der Krieken W., De Jong J.C. The formation of adventitious roots: new concepts, new possibilities. In Vitro Cell Dev Biol - Plant 1999, 35:189-199.
43. Debergh P.C., Maene L.J. A scheme for a commercial propagation of ornamental plants by tissue culture. Sci Hortic 1981, 14:335-345.
44. Dobránszki J., Jámbor-Benczúr E. Micrografting. Micrografting. Floriculture, ornamental and plant biotechnology: advances and topical issues 2006, vol. II:355-359. Global Science Books, Isleworth, UK. 1st ed. J.A. Teixeira da Silva (Ed.).
45. Dobránszki J., Abdul-Kader A., Magyar-Tábori K., Jámbor-Benczúr E., Bubán T., Szalai J., et al. In vitro shoot multiplication of apple: comparative response of three rootstocks to cytokinins and auxin. Int J Hortic Sci 2000, 6:36-39.
46. Dobránszki J., Abdul-Kader A., Magyar-Tábori K., Jámbor-Benczúr E., Bubán T., Szalai J., et al. Single and dual effects of different cytokinins on shoot multiplication of different apple scions. Int J Hortic Sci 2000, 6:76-78.
47. Dobránszki J., Magyar-Tábori K., Jámbor-Benczúr E., Lazányi J., Bubán T., Szalai J. Influence of aromatic cytokinins on shoot multiplication and their post-effects on rooting of apple cv. Húsvéti rozmaring. Int J Hortic Sci 2000, 6:84-87.
48. Dobránszki J., Magyar-Tábori K., Jámbor-Benczúr E., Lazányi J. New in vitro micrografting method for apple by sticking. Int J Hortic Sci 2000, 6:79-83.
49. Dobránszki J., Jámbor-Benczúr E., Hudák I., Magyar-Tábori K. Model experiments for establishment of in vitro culture by micrografting in apple. Int J Hortic Sci 2005, 1:47-49.
50. Driver J.A., Kuniyuki A.H. In vitro propagation of Paradox walnut rootstock. Hortic Sci 1984, 19:507-509.
51. Druart P. Optimization of culture media for in vitro rooting of Malus domestica Borkh. cv. Compact Spartan. Biol Plant 1997, 39:67-77.
52. Duron M., Paulin J.P., Brisset M.N. Use of in vitro propagated plant material for rating fire blight susceptibility. Acta Hortic 1987, 217:317-324.
53. Elliott R.F. Axenic culture of shoot apices of apple. N Z J Bot 1972, 10:254-258.
54. FAO (2009) http://faostat.fao.org/site/567/Desktopdefault.aspx?PageID=567#ancor.
55. Gahan P.B., George E.F. Adventitious regeneration. Plant propagation by tissue culture 2008, 355-401. Springer, Dordrecht, Netherlands. 3rd ed. E.F. George, M.A. Hall, G.J. De Klerk (Eds.).
56. Gamborg O.L., Miller R.A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 1968, 50:151-158.
57. Gaspar T., Kevers C., Hausman J.F., Berthon J.Y., Ripetti V. Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots. Agronomie 1992, 12:757-765.
58. Gaspar T., Kevers C., Hausman J.F., Ripetti V. Peroxidase activity and endogenous free auxin during adventitious root formation. Physiology growth and development of plant in culture 1994, 289-298. Kluwer Acadamic Publishers, The Netherlands. P.J. Lumsden, J.R. Nicholas, W.J. Davies (Eds.).
59. George E.F. Micrografting. Plant propagation by tissue culture. Part 1. 1993, 64-66. Exegetics Ltd., Edington, Wilts., UK. E.F. George (Ed.).
60. George E.F. Indirect organogenesis from callus. Plant propagation by tissue culture part 2. In practice 1996, 601-612. Exegetics Ltd., Edington, Wilts. England. 2nd ed.
61. George E.F. Fruit and nut crops. Rosaceae, Malus (apple). Plant propagation by tissue culture part 2. In practice 1996, 1039-1042. Exegetics Ltd., Edington, Wilts. England. 2nd ed.
62. George E.F. Plant tissue culture procedure - background. Plant propagation by tissue culture 2008, 1-28. Springer, Dordrecht, Netherlands. 3rd ed. E.F. George, M.A. Hall, G.J. De Klerk (Eds.).
63. George E.F., Davies W. Effects of the physical environment. Plant propagation by tissue culture 2008, 423-464. Springer, Dordrecht, Netherlands. 3rd ed. E.F. George, M.A. Hall, G.J. De Klerk (Eds.).
64. George E.F., Debergh P.C. Micropropagation: uses and methods. Plant propagation by tissue culture 2008, 29-64. Springer, Dordrecht, Netherlands. 3rd ed. E.F. George, M.A. Hall, G.J. De Klerk (Eds.).
65. Gibson S.I. Sugar and phytohormone response pathways: navigating a signalling network. J Exp Bot 2004, 55:253-264.
66. Grant N.J., Hammatt N. Increased shoot and root production during micropropagation of cherry and apple rootstocks: effect of subculture frequency. Tree Physiol 1999, 19:899-903.
67. Gupta R., Modgil M., Chakrabarti S.K. Assessment of genetic fidelity of micropropagated apple rootstock plants, AMLA 111, using RAPD markers. Indian J Exp Biol 2009, 47:925-928.
68. Hammerschlag F.A. Somaclonal variation. Biotechnology of perennial fruit crops 1996, 277-301. CAB International, Wallingford. F.A. Hammerschlag, R.E. Litz (Eds.).
69. Han X.J., Yang H.Q., Duan K.X., Zhang X.R., Zhao H.Z., You S.Z., et al. Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets. Plant Cell Tissue Organ Cult 2009, 96:29-34.
70. Hanke M.V., Flachowsky H. Fruit crops. Genetic modification of plants, biotechnology in agriculture and forestry 64 2010, 307-348. Springer-Verlag, Berlin. F. Kempken, C. Jung (Eds.).
71. Hanzawa Y., Takahashi T., Michael A.J., Burtin D., Long D., Pineiro M., et al. ACAULISS, an Arabidopsis gene required for stem elongation, encodes a spermine synthase. EMBO J 2000, 19:4248-4256.
72. Hao Y.J., Liu Q.L., Deng X.X. Effect of cryopreservation on apple genetic resources at morphological, chromosomal, and molecular levels. Cryobiology 2001, 43:46-53.
73. Hao Y.J., Zhang Z., Kitashiba H., Honda C., Ubi B., Kita M., et al. Molecular cloning and functional characterization of two apple S-adenosylmethionine decarboxylase genes and their different involvement in fruit development, cell growth and stress responses. Gene 2005, 350:41-50.
74. Harbage J.F., Stimart D.P. Effect of pH and 1H-indole-3-butyric acid (IBA) on rooting of apple microcuttings. J Am Soc Hortic Sci 1996, 121:1049-1053.
75. Harbage J.F., Stimart D.P. Ethylene does not promote adventitious root initiation on apple microcuttings. J Am Soc Hortic Sci 1996, 121:880-885.
76. Harbage J.F., Stimart D.P., Evert R.F. Anatomy of adventitious root formation in microcuttings of Malus domestica Borkh. 'Gala'. J Am Soc Hortic Sci 1993, 118:680-688.
77. 1H-indole-3-butyric acid uptake but not metabolism during the initiation phase of adventitious root induction in apple microcuttings. J Am Soc Hortic Sci 1998, 123:6-10.
78. Hevesi M., Papp J., Jámbor-Benczúr E., Kaszáné Csizmár K., Pozsgai I., Gazdag Gy., et al. Testing the virulence of some Hungarian Erwinia amylovora strains on in vitro cultured apple rootstocks. Int J Hortic Sci 2000, 6:52-55.
79. Hicks G.S. Adventitious rooting of apple microcuttings in vitro: an anatomical study. Can J Bot 1987, 65:1913-1920.
80. Hildebrandt V., Harney P.M. Factors affecting the release of phenolic exudate from explants of Pelargonium×hortorum, Bailey 'Sprinter Scarlet'. J Hortic Sci 1988, 63:651-657.
81. Huang S., Millikan D.F. In vitro micrografting of apple shoot tips. HortScience 1980, 15:741-743.
82. Huang L.C., Lee Y.L., Huang B.L. Polyphenol oxidase activity and low titratable acidity in browning bamboo tissue culture. In Vitro Cell Dev Biol - Plant 2002, 38:358-366.
83. Hutchinson J.F. Factors affecting shoot proliferation and root initiation in organ cultures of the apple Norther Spy. Sci Hortic 1984, 22:347-358.
84. Huth W. Kultur von Apfelpflanzen aus apikalen Meristem. Gartenbauwiss 1978, 43:163-166.
85. Isutsa D.K., Pritts M.P., Mudge K.W. A protocol for rooting and growing apple rootstock microshoots. Fruit Var J 1998, 52:107-116.
86. Jain S.M. Tissue culture-derived variation in crop improvement. Euphytica 2001, 118:153-166.
87. Jaiswal V.S., Amin M.N. In vitro propagation of guava from shoot cultures of mature trees. J Plant Physiol 1987, 130:7-12.
88. Jámbor-Benczúr E., Tilly-Mándy A., Szafián Zs. Effect of growth regulators on in vitro propagation of Prunus×davidopersica cv. Piroska. Med Fac Landbouww Univ Gent 1995, 60/4a:1665-1668.
89. Jámbor-Benczúr E., Neményi A., Szendrák E. Szafián Zs. In vitro propagation of Ailanthus altissima (Swingle) 'Purple Dragon'. Hortic Sci 1997, 29:22-25.
90. James D.J. Adventitious root formation 'in vitro' in apple rootstocks (Malus pumila) II. Uptake and distribution of indol-3yl-acetic acid during the auxin-sensitive phase in M.9 and M.26. Physiol Plant 1983, 57:254-258.
91. James D.J., Thurbon I.J. Rapid in vitro rooting of the apple rootstock M.9. J Hortic Sci 1979, 54:309-311.
92. James D.J., Thurbon I.J. Shoot and root initiation in vitro in the apple rootstock M.9 and the promotive effect of phloroglucinol. J Hortic Sci 1981, 56:15-20.
93. Jásik J., De Klerk G.J. Anatomical and ultrastructural examination of adventitious root formation in stem slices of apple. Biol Plant 1997, 39:79-90.
94. Jesch H.H., Plietzsch A. Field performance of in vitro propagated woody ornamentals: Prunus - I. Morphological characteristics. Gartenbauwiss 2000, 65:203-212.
95. Jesch H.H., Plietzsch A. Field performance of in vitro propagated woody ornamentals (Prunus) - II. Phenological and physiological characteristics. Gartenbauwiss 2000, 66:61-67.
96. Jones O.P. Effect of benzyladenine on isolated apple shoots. Nature 1967, 215:1514-1515.
97. Jones O.P. Effect of phloridzin and phloroglucinol on apple shoots. Nature 1976, 262:392-393.
98. Jones O.P. The role of growth regulators in the propagation in vitro (micropropagation) of temperate fruit trees. British Plant Growth Regulator Group, Monograph 13, Growth Regulators in Horticulture 1985, 113-124.
99. Jones O.P., Hadlow C.C. Juvenile-like character of apple trees produced by grafting scions and rootstocks produced by micropropagation. J Hortic Sci 1989, 64:395-401.
100. Jones O.P., Hopgood M.E., O'Farrell D. Propagation in vitro of M.26 apple rootstocks. J Hortic Sci 1977, 52:235-238.
101. Joung H., Korban S.S., Skirvin R.M. Screening shoot cultures for cedar-apple rust infection by in vitro inoculation. Plant Disease 1987, 71:1119-1122.
102. Kapoor R., Sharma D., Bhatnagar A.K. Arbuscular mycorrhizae in micropropagation systems and their potential applications. Sci Hortic 2008, 116:227-239.
103. Karhu S.T. The quality of applied carbohydrates affects the axillary branching of apple microshoots. Bull Rech Agron Gembloux 1995, 30:21-27.
104. Karhu S.T. Sugar use in relation to shoot induction by sorbitol and cytokinin in apple. J Am Soc Hortic Sci 1997, 122:476-480.
105. Karhu S.T., Ulvinen S.K. The effect of different carbohydrates on the rooting of micropropagated apple shoots and their adaptation after transplantation. Bull Rech Agron Gembloux 1995, 30:87-101.
106. Karhu S.T., Zimmerman R.H. Effect of light and coumarin during root initiation of rooting apple cultivars in vitro. Adv Hortic Sci 1993, 7:33-36.
107. Karp A. Somaclonal variation as a tool for crop improvement. Euphytica 1995, 85:295-302.
108. Kataeva N.V., Butenko R.G. Clonal micropropagation of appl trees. Acta Hortic 1987, 212:585-588.
109. Kaushal N., Modgil M., Thakur M., Sharma D.R. In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds. Indian J Exp Biol 2005, 43:561-565.
110. Korban S.S., Chen H. Apple. Biotechnology of perennial fruit crops 1992, 102-117. UK. CAB International, Wallingfrod. F.A. Hammerschlag, R.E. Litz (Eds.).
111. Kovalchuk I., Lyudvikova Y., Volgina M., Reed B.M. Medium, container and genotype all influence in vitro cold storage of apple germplasm. Plant Cell Tissue Org Cult 2009, 96:127-136.
112. Kushnarenko, S., Kovalchuk, I., Mukhitdinova, Z., Rakhimova, E., Reed, B.M., Ultrastructure study of apple meristem cells during cryopreservation. In: Turuspekov, Y., editor. Khazakistan Plant Science and Biotechnology. Asian Austral. J. Plant Sci. Biotech., in press. 4 (Special Issue 1).
113. Kushnarenko, S., Salnikov, E., Nurtazin, M., Mukhitdinova, Z., Rakhimbaev, I., Reed, B.M., Characterization and cryopreservation of Malus sieversii seeds. In: Turuspekov, Y., editor. Khazakistan Plant Science and Biotechnology. Asian Austral. J. Plant Sci. Biotech., in press. 4 (Special Issue 1).
114. Kuznetsov V.V., Shevyakova N.I. Polyamines and stress tolerance of plants. Plant Stress 2007, 1:50-71.
115. Kwon S.I., Kim J.H., Kang I.K., Kim M.J. Effects of growth regulators and culture environment on ex vitro rooting and acclimatization of apple rootstock in vitro propagated. Korean J Plant Biotech 2004, 31:133-138.
116. Lambardi M., Benelli C., Fabbri A. In vitro axillary shoot proliferation of apple rootstocks under different ethylene conditions. In Vitro Cell Dev Biol - Plant 1997, 33:70-74.
117. Lane W.D. Regeneration of apple plants from shoot meristem-tips. Plant Sci Lett 1978, 13:281-285.
118. Lane W.D., Looney N.E. A selective tissue culture medium for growth of compact (dwarf) mutants of apple. Theor Appl Genet 1982, 61:219-223.
119. Lane W.D., McDougald J.M. Shoot tissue culture of apple: comparative response of five cultivars to cytokinin and auxin. Can J Plant Sci 1982, 62:689-694.
120. Lászlóffy K., Abdul-Kader A.M., Máthé Á. In vitro propagation of Malus domestica L. cv. 'Julyred' apple. Acta Hortic 1992, 300:149-154.
121. Laukkanen H., Häggman H., Kontunen-Soppela S., Hohtola A. Tissue browning of in vitro cultures of Scots pine: Role of peroxidase and polyphenol oxidase. Physiol Plant 1999, 106:337-343.
122. Leng P., Su S., Wei F., Yi F., Duan Y. Correlation between browning, total phenolic content, polyphenol oxidase and several antioxidation enzymes during pistachio tissue culture. Acta Hortic 2009, 829:127-131.
123. Li T.H., Li S.H. Leaf responses of micropropagated apple plants to water stress: non-structural carbohydrate composition and regulatory role of metabolic enzymes. Tree Physiol 2005, 25:495-504.
124. 2-enrichment and photosynthetic photon flux affect the growth of in vitro-cultured apple plantlets. J Plant Biol 2001, 44:87-91.
125. Li X., Xu M., Korban S. DNA methylation profiles differ between field- and in vitro-grown leaves of apple. J Plant Physiol 2002, 159:1229-1234.
126. Lima-Nishimura N., Quoirin M., Naddaf Y.G., Wilhelm H.M., Ribas L.L., Sierakowski M.R. A xyloglucan from seeds of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples. Plant Cell Rep 2002, 21:402-407.
127. Linington I.M. In vitro propagation of Dipterocarpus intricatus. Plant Cell Tissue Org Cult 1991, 27:81-88.
128. Linsmayer E.M., Skoog F. Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 1965, 18:100-127.
129. Litwińczuk W. Efficiency of a double-phase medium in micropropagation of semi-dwarf apple rootstocks M.26, MM.106 and P 14. J Fruit Ornam Plant Res 2000, VIII:97-106.
130. Liu Y., Wang X., Liu L. Analysis of genetic variation in surviving apple shoots following cryopreservation by vitrification. Plant Sci 2004, 166:677-685.
131. Liu J.-H., Inoue H., Moriguchi T. Salt stress-mediated changes in free polyamine titers and expression of genes responsible for polyamine biosynthesis of apple in vitro shoots. Environ Exp Bot 2008, 62:28-35.
132. Liu J.-H., Nada K., Kurosawa T., Ban Y., Moriguchi T. Potential regulation of apple in vitro shoot growth via modulation of cellular polyamine contents. Sci Hortic 2009, 119:423-429.
133. Lloyd G., McCown B. Commercially feasible micropropagation of mountain laurel Kalmialatifolia by use of shoot tip-culture. Int Plant Prop Soc Proc 1981, 30:421-427.
134. Loescher W.H., Everard J.D. Sugar alcohol metabolism in sinks and sources. Photoassimilate distribution in plants and crops: source-sink relationships 1996, 185-207. Marcel Dekker, New York. E. Zamski, A.A. Schaffer (Eds.).
135. Lucyszyn N., Quoirin M., Anjos A., Sierakowski M.R. Blends of agar/galactomannan for Marubakaido apple rootstock shoot proliferation. Polímeros Ciên Tecnol 2005, 15:146-150.
136. Luo J., Reed B.M. Abscisic acid-responsive protein, bovine serum albumin, and proline pretreatments improve recovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrification. Cryobiology 1997, 34:240-250.
137. M'Kada J., Dorin N., Bigot C. In vitro propagation of Arctostaphylos uvaursi L. Sprengel.: comparison between two methodologies. Plant Cell Tissue Org Cult 1991, 24:217-222.
138. Ma J.H., Yao J.L., Cohen D., Morris B. Ethylene inhibitors enhance in vitro root formation from apple shoot cultures. Plant Cell Rep 1998, 17:211-214.
139. Magyar-Tábori K., Dobránszki J., Jámbor-Benczúr E., Lazányi J. Role of cytokinins in shoot proliferation of apple in vitro. Analele Universitţ ii din Oradea VII. Partea I Fascicula Agricultur Şi Horticultur ; 2001, 17-24.
140. Magyar-Tábori K., Dobránszki J., Jámbor-Benczúr E., Bubán T., Lazányi J., Szalai J., et al. Post-effects of cytokinins and auxin levels of proliferation media on rooting ability of in vitro apple shoots (Malus domestica Borkh.) 'Red Fuji'. Int J Hortic Sci 2001, 7:26-29.
141. Magyar-Tábori K., Dobránszki J., Jámbor-Benczúr E., Lazányi J., Szalai J. Effects of activated charcoal on rooting of in vitro apple (Malus domestica Borkh.) shoots. Int J Hortic Sci 2001, 7:98-101.
142. Magyar-Tábori K., Dobránszki J., Jámbor-Benczúr E. High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues. Acta Agron Hung 2002, 50:191-195.
143. Magyar-Tábori K., Dobránszki J., Jámbor-Benczúr E., Lazányi J., Szalai J., Ferenczy A. Effects of indole-3-butyric acid levels and activated charcoal on rooting of in vitro shoots of apple rootstocks. Int J Hortic Sci 2002, 8:25-28.
144. Magyar-Tábori K., Dobránszki J., Hudák I. Factors affecting the rooting ability of in vitro apple shoots. Hung Agric Res 2009, 18:10-14.
145. Magyar-Tábori, K., Dobránszki, J., Teixeira da Silva, J.A., Bulley, S.M., Hudák, I., The role of cytokinins in shoot organogenesis in apple. Plant Cell Tissue Org Cult in press. doi:10.1007/s11240-010-9696-6.
146. Marga F., Vebret L., Morvan H. Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity. Plant Cell Tissue Org Cult 1997, 49:1-5.
147. Marin J.A., Jones O.P., Hadlow W.C.C. Micropropagation of columnar apple trees. J Hortic Sci 1993, 68:289-297.
148. Mato M.C., Mendez J. Inhibition of indoleacetic acid-oxidase by sodium humate. Geoderma 1970, 3:255-258.
149. Matsumoto T., Sakai A. Cryopreservation of grape in vitro-cultured axillary shoot tips by three-step vitrification. Cryopreservation of tropical plant germplasm. Current Research Progress and Application, Japan International Research Center for Agricultural Sciences and International Plant Genetic Resources Institute, Rome 2000, 424-425. F. Engelmann, H. Takagi (Eds.).
150. McMeans O., Skirvin R.M., Otterbacher A., Mitiku G. Assessment of tissue culture-derived 'Gala' and 'Royal Gala' apples (Malus x domestica Borkh.) for somaclonal variation. Euphytica 1998, 103:251-257.
151. Micheli M., Pellegrino S., Piccioni E., Standardi A. Effects of double encapsulation and coating on synthetic seed conversion in M.26 apple rootstock. J Microencapsul 2002, 19:347-356.
152. Modgil M., Sharma D.R., Bhardwaj S.V. Micropropagation of apple cv. Tydeman Early Worcester. Sci Hortic 1999, 81:179-188.
153. Modgil M., Mahajan K., Chakrabarti S.K., Sharma D.R., Sobti R.C. Molecular analysis of genetic stability in micropropagated apple rootstock MM106. Sci Hortic 2005, 104:151-160.
154. Modgil M., Sharma T., Thakur M. Commercially feasible protocol for rooting and acclimatization of micropropagated apple rootstocks. Acta Hortic 2009, 839:209-214.
155. Mohamed A.E.-S. Somaclonal variation in micro-propagated strawberry detected at the molecular level. Int J Agric Biol 2007, 9:721-725.
156. Mohan R., Soccol C.R., Quoirin M., Pandey A. Use of sugarcane bagasse as an alternative low-cost support material during the rooting stage of apple micropropagation. In Vitro Cell Dev Biol - Plant 2004, 40:408-411.
157. Molassiotis A., Sotiropoulos T., Tanou G., Diamantidis G., Therios I. Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environ Exp Bot 2006, 56:54-62.
158. Moshkov I.E., Novikova G.V., Hall M.A., George E.F. Plant growth regulators iii: gibberellins, ethylene, abscisic acid, their analogues and inhibitors; miscellaneous compounds. Plant propagation by tissue culture 2008, 227-281. Springer, Dordrecht, Netherlands. 3rd ed. E.F. George, M.A. Hall, G.J. De Klerk (Eds.).
159. Mouhtaridou G.N., Sotiropoulos T.E., Dimassi K.N., Therios I.N. Effects of boron on growth, and chlorophyll and mineral contents of shoots of the apple rootstock MM 106 cultured in vitro. Biol Plant 2004, 48:617-619.
160. Muleo R., Morini S. Light quality regulates shoot cluster growth and development of MM 106 apple genotype in in vitro culture. Sci Hortic 2006, 108:364-370.
161. Muleo R., Morini S. Physiological dissection of blue and red light regulation of apical dominance and branching in M9 apple rootstock growing in vitro. J Plant Physiol 2008, 165:1838-1846.
162. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962, 15:473-497.
163. Murata M., Tsurutani M., Hagiwara S., Homma S. Subcellular location of polyphenol oxidase in apples. Biosci Biotech Biochem 1997, 61:1459-1499.
164. Nabeela A.B., Darkazanli K., Abdul-Kader A.M. Direct organogenesis and plantlet multiplication from leaf explants of in vitro-grown shoots of apple (Malus domestica Borkh.) cv. 'Golden Delicious' and MM111 rootstock. Fruit Veg Cereal Sci Biotech 2009, 3:28-34.
165. Naija S., Elloumi N., Jbir N., Ammar S., Kevers C. Anatomical and biochemical changes during adventitious rooting of apple rootstocks MM 106 cultured in vitro. C R Biol 2008, 331:518-525.
166. Naija S., Elloumi N., Ammar S., Kevers C., Dommes J. Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106. In Vitro Cell Dev Biol - Plant 2009, 45:83-91.
167. Nguyen T.V., Thu T.T., Claeys M., Angenon G. Agrobacterium mediated transformation of sorghum (Sorghum bicolour (L.) Moench) using an improved in vitro regeneration system. Plant Cell Tissue Org Cult 2007, 91:155-164.
168. Nhut D.T., Teixeira da Silva J.A., Van Le B., Thorpe T., Tran Thanh Van K. Woody plant micropropagation and morphogenesis by thin cell layers. Thin cell layer culture system. Regeneration and transformation applications 2003, 473-493. Kluwer Academic Publishers, The Netherlands. D.T. Nhut, J.A. Teixeira da Silva, B. Van Le, K. Tran Thanh Van, T. Thorpe (Eds.).
169. Noiraud N., Maurousset L., Lemoine R. Transport of polyols in higher plants. Plant Physiol Biochem 2001, 39:717-728.
170. Noiton D., Vine J.H., Mullins M.G. Effects of serial subculture in vitro on the endogenous levels of indole-3-acetic acid and abscisic acid and rootability in micro-cuttings of 'Jonathan' apple. Plant Growth Regul 1992, 11:377-383.
171. O'Herlihy E.A., Croke J.T., Cassells A.C. Influence of in vitro factors on titre and elimination of model fruit tree viruses. Plant Cell Tissue Org Cult 2003, 72:33-42.
172. Onay A., Jeffree C.E. Somatic embryogenesisin pistachio (Pistacia vera L.). Somatic embryogenesis in woody plants 2000, vol. 6:361-390. Kluwer Academic Publishers, The Netherlands.
173. Paek K.Y., Chakrabarty D., Hahn E.J. Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tissue Org Cult 2005, 81:287-300.
174. Pan M.J., van Staden J. The use of charcoal in in vitro culture - a review. Plant Growth Regul 1998, 26:155-163.
175. Pang X.-M., Zhang Z.-Y., Wen X.-P., Ban Y., Moriguchi T. Polyamines, all-purpose players in response to environment stresses in plants. Plant Stress 2007, 1:173-188.
176. Paprstein F., Sedlak J., Polak J., Svoboda L., Hassan M., Bryxiova M. Results of in vitro thermotherapy of apple cultivars. Plant Cell Tissue Org Cult 2008, 94:347-352.
177. Pasqualetto P.-L., Zimmerman R.H., Fordham I.J. The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tissue Org Cult 1988, 14:31-40.
178. Pawlicki N., Welander M. Influence of carbohydrate source, auxin concentration and time to exposure on adventitious rooting of the apple rootstock Jork 9. Plant Sci 1995, 106:167-176.
179. Peil A., Bus V.G.M., Geider K., Richter K., Flachowsky H., Hanke M.-V. Improvement of fire blight resistance in apple and pear. Int J Plant Breed 2009, 3:1-27.
180. Pereira Cavallazzi J.R., Klauberg Filho O., Stürmer S.L., Rygiewicz P.T., Matos de Mendonca M. Screening and selecting arbuscular mycorrhizal fungi for inoculating micropropagated apple rootstocks in acid soils. Plant Cell Tissue Org Cult 2007, 90:117-129.
181. Pereira-Netto A.B., Petkowitz C.L.O., Cruz-Silva C.T.A., Gazzoni M.T., Mello A.F.P., Silveira J.L.M. Differential performance of marubakaido apple rootstock grown in culture media containing different agar brands: dynamic rheological analysis. In Vitro Cell Dev Biol - Plant 2007, 43:356-363.
182. Pérez-Tornero O., Burgos L. Apricot micropropagation. Protocols for micropropagation of woody trees and fruits 2007, 267-278. Springer, Dordrecht, The Netherlands. S.M. Jain, H. Häggman (Eds.).
183. Piccioni E. Plantlets from encapsulated micropropagated buds of M.26 apple rootstock. Plant Cell Tissue Org Cult 1997, 47:255-260.
184. Pierik R.L.M. Commercial aspects of micropropagation. Horticulture - new technologies and applications 1991, 141-153. Kluwer Academic Publisher, Dordrecht, The Netherlands. J. Prakash, R.L.M. Pierik (Eds.).
185. Pontaroli A.C., Camadro E.L. Plant regeneration after long term callus culture in clones of Asparagus officinalis L. Biocell 2005, 29:313-317.
186. 2 enrichment effect on in vitro grown apple and kiwifruit. Acta Hortic 1991, 300:107-110.
187. Preece J.E. Novel methods in micropropagation. 1st international symposium on 'acclimatization and establishment of micropropagated plants'. Sani-Halkidiki, Greece, 19-22 September 2001, 6.
188. Preece J.E., Read P.E. Novel methods in micropropagation. Acta Hortic 2003, 616:71-76.
189. Pua E.-C., Chong C. Requirement for sorbitol (d-glucitol) as carbon source for in vitro propagation of Malus robusta no. 5. Can J Bot 1984, 62:1545-1549.
190. Pua E.-C., Chong C. Regulation of in vitro shoot and root regeneration in 'Macspur' apple by sorbitol (d-glucitol) and related carbon sources. J Am Soc Hortic Sci 1985, 110:705-709.
191. Puente J.P., Marin J.A. In vitro rootability of clonal apple microcuttings, derived from rooted and unrooted shoots. Sci Hortic 1997, 68:227-230.
192. Quorin M., Lepoivre P. Improved media for in vitro culture of Prunus sp. Acta Hortic 1977, 78:437-442.
193. Quoirin M, Lepoivre P, Boxus P. Un Premier Bilan de 10 Années de Recherches sur les Cultures de Méristèmes et la Multiplication 'In Vitro' de Fruitiers Ligneux. Compte rendu des Réchérches 1976-1977 at rapports de synthèse de la Station des Cultures fruitières et maraicheres Centre de Réchérches Agronomiques de l'Etat B 58000 Gembloux; 1977. p. 93-117.
194. Rai M.K. Current advances in mycorrhization in micropropagation. In Vitro Cell Dev Biol - Plant 2001, 37:158-167.
195. Reinert J., Backs D. Control of totipotency in plant cells growing in vitro. Nature 1968, 220:1340-1341.
196. Ricci A., Carra A., Maggiali C.A., Incerti M., Branca C. Weakly cytokinin-active diphenylurea derivates influence adventitious rooting in M26 Malus pumila microcuttings. Plant Growth Regul 2003, 39:19-26.
197. Ricci A., Carra A., Torelli A., Maggiali C.A., Morini G., Branca C. Cytokinin-like activity of N, N'-diphenylureas. N, N'-bis-(2, 3-methylenedioxyphenyl)urea and N, N'-bis-(3, 4- methylenedioxyphenyl)urea enhance adventitious root formation in apple rootstock M26 (Malus pumila Mill.). Plant Sci 2001, 160:1055-1065.
198. Ríoz Leal D., Sánchez-Olate M., Avilés F., Materan M.E., Uribe M., Hasbún R., et al. Micropropagation of Juglans regia L. Protocols for micropropagation of woody trees and fruits 2007, 381-390. Springer, Dordrecht, The Netherlands. S.M. Jain, H. Häggman (Eds.).
199. Sarwar M., Skirvin R.M., Kushad M., Norton M.A. Selecting dwarf apple (Malus x domestica Borkh.) trees in vitro: multiple cytokinin tolerance expressed among three strains of 'McIntosh' that differ in their growth habit under field conditions. Plant Cell Tissue Org Cult 1998, 54:71-76.
200. Schaefer S., Medeiro S.A., Ramírez J.A., Galagovsky L.R., Pereira-Netto A.B. Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Rep 2002, 20:1093-1097.
201. Schubert A., Lubraco G. Mycorrhizal inoculation enhances growth and nutrient uptake of micropropagated apple rootstocks during weaning in commercial substrates of high nutrient availability. Appl Soil Ecol 2000, 15:113-118.
202. Sedira M., Butler E., Gallagher T., Welander M. Verification of auxin-induced gene expression during adventitious rooting in rolB-transformed and untransformed apple Jork 9. Plant Sci 2005, 168:1193-1198.
203. Sedira M., Welander M., Geier T. Influence of IBA and aphidicolin on DNA synthesis and adventitious root regeneration from Malus 'Jork 9' stem discs. Plant Cell Rep 2007, 26:539-545.
204. Seifert G., Kanzler P., da Câmara Machado A., Katinder H., Laimer da Câmara Machado M. Hormonal optimization and morphological characterization of the M9 Jork stem and disc system. Towards its biochemical characterization. Adv Hortic Sci 1994, 8:61-66.
205. Sharma R.R., Singh S.K. Etiolation reduces phenolic content and polyphenol oxidase activity at the pre-culture stage and in-vitro exudation of phenols from mango explants. Trop Agric 2002, 79:94-99.
206. Sharma M., Modgil M., Sharma D.R. Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol. Indian J Exp Biol 2000, 38:1236-1240.
207. Sicurani M., Piccioni E., Standardi A. Micropropagation and preparation of synthetic seed in M@26 apple rootstock I: attempts towards saving labor in the production of adventitious shoot tips suitable for encapsulation. Plant Cell Tissue Org Cult 2001, 66:207-216.
208. Skirvin R.M., Norton M., McPheeters K.D. Somaclonal variation: has it proved useful for plant improvement?. Acta Hortic 1993, 336:333-340.
209. Snir I., Erez A. In vitro propagation of Malling Merton apple rootstocks. HortScience 1980, 15:597-598.
210. Son S.H., Moon H.K., Hall R.B. Somaclonal variation in plants regenerated from callus culture of hybrid aspen (Populus alba L. x P. grandidentata Michx.). Plant Sci 1993, 90:89-94.
211. Sotiropoulos T.E., Molassiotis A.N., Mouhteridou G.I., Papadakis I., Dimassi K.N., Therios I.N., et al. Sucrose and sorbitol effects on shoot growth and proliferation in vitro, nutritional status and peroxidise and catalase isoenzymes of M 9 and MM 106 (Malus domestica Borkh.) rootstocks. Eur J Hortic Sci 2006, 71:114-119.
212. Soumelidou K., Battey N.H., John P., Barnett J.R. The anatomy of the developing bud union and its relationship to dwarfing in apple. Ann Bot 1994, 74:605-611.
213. Souza J.A., Donini L.P., Silva L.C., Corrêa M.G.S., Schuch M.W. Entraizamento in vitro do porta-enxerto de macieira - M9 em função da vedação, sacarose e material de suporte no meio de cultura. Sci Agric 2007, 8:161-164.
214. Sriskandarajah S., Mullins M.G. Micropropagation of Granny Smith apple: factors affecting root formation in vitro. J Hortic Sci 1981, 56:71-76.
215. Sriskandarajah S., Mullins M.G., Nair Y. Induction of adventitious rooting in vitro in difficult-to-propagate cultivars of apple. Plant Sci Lett 1982, 24:1-9.
216. Sriskandarajah S., Skirvin R.M., Abu-Qaoud H., Korban S.S. Factors involved in shoot elongation and growth of adventitious and axillary shoots of three apple scion cultivars in vitro. J Hortic Sci 1990, 65:113-121.
217. Sriskandarajah S., Skirvin R.M., Abu-Qaoud H. The effect of some macronutrients on adventitious root development on scion apple cultivars in vitro. Plant Cell Tissue Org Cult 1990, 21:185-189.
218. Staba E.J. Plant tissue culture as a technique for the phytochemist. Recent advances in phytochemistry 1969, vol. 2:75-102. Appleton-Century Crofts, New York.
219. Tantos Á., Mészáros A., Farkas T., Szalai J., Horváth G. Triacontanol-supported micropropagation of woody plants. Plant Cell Rep 2001, 20:16-21.
220. Teisson C., Alvard D. A new concept of plant in vitro cultivation in liquid medium: temporary immersion. Current issues in plant molecular and cellular biology 1995, 105-110. Kluwer Academic Publishers, Dordrecht. M. Terzi (Ed.).
221. Thomas T.D. The role of activated charcoal in plant tissue culture. Biotechnol Adv 2008, 26:618-631.
222. Thorpe T., Stasolla C., Yeung E.C., De Klerk G.-J., Roberts A., George E.F. The components of plant tissue culture media ii: organic additions, osmotic and pH effects, and support systems. Plant propagation by tissue culture 2008, 115-173. Springer, Dordrecht, Netherlands. 3rd ed. E.F. George, M.A. Hall, G.J. De Klerk (Eds.).
223. Tisserat B. Propagation of data palm (Phoenix dactylifera L.) in vitro. J Exp Bot 1979, 30:1275-1283.
224. Van der Krieken W.M., Breteler H., Visser M.H.M., Mavridou D. The role of conversation of IBA into IAA on root regeneration in apple: introduction of a test system. Plant Cell Rep 1993, 12:203-206.
225. Van Nieuwkerk J.P., Zimmerman R.H., Fordham I. Thidiazuron stimulation of apple shoot proliferation in vitro. HortScience 1986, 21:516-518.
226. Vasil I.K., Vasil V. Totipotency and embryogenesis in plant cell and tissue cultures. In Vitro Cell Dev Biol - Plant 1972, 8:117-125.
227. Végváry G., Vitányi G., Mészáros A., Lelik L. Determination of benzyladenine content of in vitro propagated dwarf J-TE-F apple rootstock (Malus sp.) by high performance liquid chromatography. Gartenbauwissenschaft 2000, 65:241-244.
228. Verdeil J.-L., Alemanno L., Niemenak N., Tranbarger T.J. Pluripotent versus totipotent plant stem cells: dependence versus autonomy?. Trends Plant Sci 2007, 12:245-252.
229. Walkey D.G. Production of apple plantlets from axillary-bud meristem. Can J Plant Sci 1972, 52:1085-1087.
230. Wang P.J., Charles A. Micropropagation through meristem culture. Biotechnology in agriculture and forestry 1991, vol. 17:32-53. Springer, Berlin.
231. Wang Q., Tang H., Quan Y., Zhou G. Phenol induced browning and establishment of shoot-tip explants of 'Fuji' apple and 'Jinhua' pear cultured in vitro. J Hortic Sci 1994, 69:833-839.
232. Ward S.P., Leyser O. Shoot branching. Curr Opin Plant Biol 2004, 7:73-78.
233. Watillon B., Kettmann R., Boxus P., Burny A. Characterization of two gene transcripts modulated by cytokinins in micropropagated apple (Malus domestica [L.] Borkh) plantlets. Plant Physiol 1991, 96:479-484.
234. Webster C.A., Jones O.P. Micropropagation of apple rootstock M9: Effect of sustained subculture on apparent rejuvenization in vitro. J Hortic Sci 1989, 64:421-428.
235. Webster C.A., Jones O.P. Micropropagation of some cold hardy dwarfing rootstocks for apple. J Hortic Sci 1991, 66:1-6.
236. Welander M. In vitro shoot and root formation in the apple cultivar Åkerö. Ann Bot 1985, 55:249-261.
237. Welander M. Regulation of in vitro shoot multiplication in Syringa, Alnus and Malus by different carbon sources. J Hortic Sci 1989, 64:361-366.
238. Welander M., Pawlicki N. A model system for studying root regeneration in woody species. Acta Hortic 1993, 336:225-230.
239. Welander M., Zhu L.H., Li X.Y. Factors influencing conventional and semi-automated micropropagation. Propag Ornam Plant 2007, 7:103-111.
240. Werner E.M., Boe A.A. In vitro propagation of Malling 7 apple rootstock. HortScience 1980, 15:509-510.
241. Yepes L.M., Aldwinckle H.S. Micropropagation of thirteen Malus cultivars and rootstocks, and effect of antibiotics on proliferation. Plant Growth Regul 1994, 15:55-67.
242. Zaid S., Soulaiman M., Abdul-Kader A. In vitro propagation of three apple rootstocks. Damascus Uni J Basic Sci 2000, 16(2):63-78.
243. Zanandrea I., Bacarin M.A., Schmitz D.D., Braga E.J.B., Peters J.A. Chlorophyll fluorescence in in vitro cultivated apple. Rev Bras Agroci Pelotas 2006, 12:305-308.
244. Zeller J.K., Larsen F.E., Higgins S.S., Curry E.A. Rootstock effects on responses of potted 'Smoothee Golden Delicious' apple to soil-applied triazole growth inhibitors. I: Shoot and root growth. Sci Hortic 1991, 46:61-74.
245. Zhou R., Cheng L., Dandekar A.M. Down-regulation of sorbitol dehydrogenase and up-regulation of sucrose synthase in shoot tips of the transgenic apple trees with decreased sorbitol synthesis. J Exp Bot 2006, 57:3647-3657.
246. Zhu L.H., Li X.Y., Welander M. Micropropagation of the apple rootstock M26 by temporary immersion system (TIS). Acta Hortic 2003, 81:313-318.
247. Zhu L.H., Li X.Y., Welander M. Optimisation of growing conditions for the apple rootstock M26 grown in RITA containers using temporary immersion principle. Plant Cell Tissue Org Cult 2005, 81:313-318.
248. Zimmerman R.H. Micropropagation of temperate zone fruit and nut crops. Micropropagation 1991, 231-246. Kluwer Academic Publishers, Dordrecht. P.C. Debergh, R.H. Zimmerman (Eds.).
249. Zimmerman R.H., Debergh P.C. Micropropagation of temperate zone fruit and nut crops. Micropropagation: technology and application 1991, 231-246. Kluwer Academic, Boston. R.H. Zimmerman, P.C. Debergh (Eds.).
250. Zimmerman R.H., Fordham I.M. Simplified method for rooting apple cultivars in vitro. J Am Soc Hortic Sci 1985, 110:34-38.
251. Zimmerman R.H., Bhardwaj S.V., Fordham I.M. Use of starch-gelled medium for tissue culture of some fruit crops. Plant Cell Tissue Org Cult 1995, 43:207-213.
252. Ziv M., Halevy A.H. Control of oxidative browning and in vitro propagation of Strelitzia reginae. HortScience 1983, 18:1085-1087.