Production and characterization of a single-chain Fv antibody-alkaline phosphatase fusion protein specific for clenbuterol

Molecular Biotechnology

Volumn 45, Issue 1, 2010, Pages 56-64

  Liu, Xixia ^a   Wang, Hong ^a   Liang, Yan ^a   Yang, Jinyi ^a   Zhang, Hongbin ^b   Lei, Hongtao ^a   Shen, Yudong ^a   Sun, Yuanming ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND   (China)

Author keywords

Bacterial alkaline phosphatase; Clenbuterol; ELISA; Fusion protein; Single chain Fv antibody

Indexed keywords

ALKALINE PHOSPHATASE; CLENBUTEROL; FUSION PROTEIN; FUSION PROTEINS; SINGLE-CHAIN FV;

BACTERIOLOGY; CLONING; ENZYME INHIBITION; ESCHERICHIA COLI; GENE EXPRESSION; MONOCLONAL ANTIBODIES; PHOSPHATASES;

PROTEINS;

ALKALINE PHOSPHATASE; BACTERIAL DNA; BACTERIAL ENZYME; CLENBUTEROL; HYBRID PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CROSS REACTION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE INSERTION; IC 50; MICHAELIS MENTEN KINETICS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN BINDING; WESTERN BLOTTING;

ALKALINE PHOSPHATASE; ANTIBODIES, MONOCLONAL; CLENBUTEROL; HUMANS; IMMUNOASSAY; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77954818994     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9240-2     Document Type: Article

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