Development of a polyclonal indirect ELISA with sub-ng g-1 sensitivity for the analysis of clenbuterol in milk, animal feed, and liver samples and a small survey of residues in retail animal products

Food Additives and Contaminants - Part A

Volumn 26, Issue 8, 2009, Pages 1153-1161

  He, L ^a   Pu, C ^a   Yang, H ^a   Zhao, D ^a   Deng, A P ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Clenbuterol; Enzyme linked immunosorbent assay (ELISA); Food sample; Polyclonal antibody; agonist

Indexed keywords

ADRENALIN; CHLORAMPHENICOL; CLENBUTEROL; ESTRONE; LEVONORGESTREL; METHYLPREDNISOLONE; METRONIDAZOLE; OLAQUINDOX; PHENYLEPHRINE; POLYCLONAL ANTIBODY; RACTOPAMINE; SALBUTAMOL;

ANIMAL FOOD; ANIMAL PRODUCT; ANIMAL TISSUE; ARTICLE; CHICKEN; CONTROLLED STUDY; CROSS REACTION; DRUG STRUCTURE; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOD ANALYSIS; FOOD CONTAMINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; LIVER PARENCHYMA; MILK; NONHUMAN; PRIORITY JOURNAL; RABBIT; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SWINE;

ANIMALIA; ORYCTOLAGUS CUNICULUS; SUIDAE;

EID: 70849135665     PISSN: 19440049     EISSN: 14645122     Source Type: Journal    
DOI: 10.1080/02652030902906142     Document Type: Article

References (36)

1. Blanca J, Munoz P, Morgado M, Mendez N, Aranda A, Reuvers T, Hooghuis H. 2005. Determination of clenbuterol, ractoamine and zilpaterol in liver and urine by liquid chromatography tandem mass spectrometry. Analyt Chimica Acta. 529:199-205.
2. Blass A, Dave M, Fallon RJ, Illera JC, Illera M, Sauer MJ. 1999. Determination of therapeutic and growth-promoting use of clenbuterol by plasma analysis. J Vet Pharmacol Ther. 22:234-237.
3. Blomgren A, Berggren C, Holmberg A, Larsson F, Sellergren B, Ensing K. 2002. Extraction of clenbuterol from calf urine using a molecularly imprinted polymer followed by quantitation by high-performance liquid chromatography with UV detection. J Chromatograph A. 975:157-164.
4. Brambilla G, Cenci T, Franconi F, Galarini R, Macri A, Rodoni F, Strozzi M, Loizzo A. 2000. Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy. Toxicol Lett. 114:47-53.
5. Castro F, Carcia MC, Rodriguez R, Rodriguez J, Marina ML. 2007. Determination of soybean proteins in commercial heat-processed meat products prepared with chicken, beef or complex mixtures of meat from different species. Food Chem. 100:468-476.
6. Chan TYK. 1999. Health hazards due to clenbuterol residues in food. J Toxicol Clin Toxicol. 37:517-519.
7. Chan TYK. 2001. Food-borne clenbuterol may have potential for cardiovascular effects with chronic exposure. J Toxicol Clin Toxicol. 39:345-348.
8. Chinese National Standard. 2000. Chinese National Standard, GB-16869-2000.
9. Chinese National Standard. 2001. Chinese National Standard, GB18406.3-2001.
10. Clarkson PM, Thompson HS. 1997. Drugs and sport. Research findings and limitations. Sport Med. 24:366-384.
11. Degand G, Bernes-Duyckaerts A, Maghuin-Rogister G. 1992. Determination of clenbuterol in bovine tissue and urine by enzyme immunoassay. J Agricult Food Chem. 40:70-75.
12. Deng AP, Kolar V, Franek M. 2002. Direct competitive ELISA for the detection of polychlorinated biphenyls in soil samples. Analyt Bioanalyt Chem. 373:685-690.
13. Elliott CT, McCaughey WJ, Shortt HD. 1993. Residues of the beta-agonist clenbuterol in tissues of medicated farm animals. Food Addit Contam. 10:231-244.
14. Haasnoot W, Streppel L, Cazemier G, Salden M, Stouten P, Essers M, Van Wichen P. 1996. Development of a tube enzyme immunoassay for on-site screening of urine sample in the presence of beta-agonists. Analyst. 121:1111-1114.
15. Hahnau S, Julicher B. 1996. Evaluation of commercially available ELISA test kits for the detection of clenbuterol an other beta 2-agonists. Food Addit Contam. 13:259-274.
16. Harkins JD, Woods WE, Lehner AF, Fisher M, Tobin T. 2001. Clenbuterol in the horse: urinary concentrations determined by ELISA and GC/MS after clinical doses. J Vet Pharmacol Ther. 24:7-14.
17. Ji XH, He ZK, Ai XP, Yang HX, Xu CL. 2006. Determination of clenbuterol by capillary electrophoresis immunoassay with chemiluminescence detection. Talanta. 70:353-357.
18. Johansson MA, Hellenas KE. 2001. Sensor chip preparation and assay construction for immunobiosensor determination of beta-agonists and normones. Analyst. 126: 1721-1727.
19. Knopp D. 2006. Detection of trace metal ions in environmental and biological matrices by immunological methods - a review. Ecolog Chem Eng. 13:383-397.
20. Koole A, Bosman B, Franke JP, de Zeeuw RA. 1999. Multiresidue analysis of 2-agonists in human and calf urine using multimodal solid-phase extraction and high-performance liquid chromatography with electrochemical detection. J Chromatograph B. 726:149-156.
21. Kuiper HA, Noordam MY, Van Dooren-Flipsen MMH, Schilt R, Roos AH. 1998. Illegal use of -adrenergic agonists: European Community. Czech J Anim Sci. 76:195-207.
22. Matschulat D, Deng AP, Niessner R, Knopp D. 2005. Development of a highly sensitive monoclonal antibody based ELISA for detection of benzo[a] pyrene in potable water. Analyst. 130:1078-1086.
23. Matsumoto M, Maruyama N, Watanabe H, Kikuchihara T, Tsuji A. 2000. Residue analysis of clenbuterol in bovine and horse tissues and cow's milk by enzyme-linked immunosorbent assay. J Food Hyg Soc Jpn. 41:48-53.
24. Mitchell GA, Dunnavan G. 1998. Illegal use of -adrenergic agonists in the United States. J Anim Sci. 76:208-211.
25. Petruzzelli E, Ius A, Berta S, Dovis M, Albertini A. 1996. Preparation and characterization of a monoclonal anti- body specific for the beta-agonist clenbuterol. Food Agricult Immunol. 8:3-10.
26. Posyniak A, Zmudzki J, Niedzielska J. 2003. Screening procedures for clenbuterol residue determination in bovine urine and liver matrices using enzyme-linked immunosorbent assay and liquid chromatography. Analyt Chim Acta. 483: 61-67.
27. Prather ID, Brown DE, North P, Wilson JR. 1995. Clenbuterol - a substitute for anabolic-steroids. Med Sci Sport Exer. 27:1118-1121.
28. Prezelj A, Obreza A, Pwcar S. 2003. Abuse of clenbuterol and its detection. Curr Med Chem. 10:281-290.
29. Pulce C, Lamaison D, Keck G, Bostvironnois C, Nicolas J, Descotes J. 1991. Collective human food poisonings by clenbuterol residues in veal liver. Vet Hum Toxicol. 33:480-481.
30. Roda A, Manetta AC, Piazza F, Simony P, Lelli R. 2000. A rapid and sensitive 284-microtiter wells format chemiluminescent enzyme immunoassay for clenbuterol. Talanta. 52:311-318.
31. Sawaya WN, Lone K, Husain A, Dashti B, Saeed T. 2000. Screening for beta-agonists in sheep urine and eyes by an enzyme-linked immunosorbent in the state of Kuwait. Food Contr. 11:1-5.
32. Wang XN, Zhang GP. 2004. Illegal use and safety evaluation of clenbuterol in China. Chin J Anim Sci. 40:52.
33. Xu T, Wang BM, Sheng W, Li QX, Shao XL, Li J. 2007. Application of an enzyme-linked immunosorbent assay for the detection of clenbuterol in swine urine and feeds. J Environ Sci Hlth A. 42:173-177.
34. Zhang GP, Wang XN, Yang JF, Yang YY, Xing GX, Li QM, Zhao D, Chai SJ, Guo JQ. 2006. Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist clenbuterol residues. J Immunol Meth. 312:27-33.
35. Zhou JY, Xu XS, Wang YX. 2007. Competitive immunoas- say for clenbuterol using capillary electrophoresis with laser-induced fluorescence detection. J Chromatograph B. 848:226-231.
36. Zuo P, Ye BC. 2006. Small molecule microarrays for drug residue detection in foodstuffs. J Agricult Food Chem. 54:6978-6983.