Production, purification and characterisation of genetically derived scFv and bifunctional antibody fragments capable of detecting illicit drug residues

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 786, Issue 1-2, 2003, Pages 327-342

  Brennan, Joanne ^a   Dillon, Paul ^a   O’Kennedy, Richard ^a  

^a DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

BIAcore; Expression vectors; Purification; ScFv

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; DRUG PRODUCTS; ENZYMES; GENES; IMMUNIZATION; PURIFICATION;

BIFUNCTIONAL MOLECULES;

ANTIBODIES;

ALKALINE PHOSPHATASE; ANTIBODY; ANTIGEN; DRUG RESIDUE; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; LINK PROTEIN; MORPHINE; SEPHAROSE; SINGLE CHAIN ANTIGEN BINDING PROTEIN; UNCLASSIFIED DRUG;

ANALYSIS; ANTIGEN BINDING; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; BINDING AFFINITY; CONFERENCE PAPER; DIMERIZATION; DNA VECTOR; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE; GENE ISOLATION; GENETIC CODE; IMMUNOAFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; SALIVA; SAMPLE; WESTERN BLOTTING;

BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; DRUG RESIDUES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN FRAGMENTS; PLASMIDS; STREET DRUGS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MURINAE; VECTORS;

EID: 0037465675     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-0232(02)00807-3     Document Type: Article

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